LOCUS       BBa_K2170011            1704 bp    DNA     linear       22-Nov-2024
DEFINITION  Secretory Nanoluciferase with C-terminal StrepTag 
            (CMV_EGFR_NanoLuc_StrepTag_polyA)
ACCESSION   BBa_K2170011
VERSION     BBa_K2170011.1  
FEATURES             Location/Qualifiers
     promoter        1..559
                     /label=CMV
     CDS             596..667
                     /label=EGFR signal peptide
     CDS             674..1183
                     /label=NanoLuc
     misc_feature    1190..1213
                     /label=Strep TagII
     polyA_site      1224..1704
                     /label=polyA
ORIGIN
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagatgg ccggcatgag
      601 accatctggt actgctggag ccgcattgct ggcacttttg gctgcgctgt gccctgcaag
      661 cagagcaacc ggcgtgttca ccctcgagga tttcgttgga gattggagac agaccgctgg
      721 atacaacctt gatcaggttt tggagcaggg tggtgtgtct agtcttttcc agaacctcgg
      781 agtgtctgtg acccctatcc agagaatcgt tctctctggt gagaacggac tcaagatcga
      841 tatccacgtg atcatccctt acgagggact ctcaggtgat cagatgggac agatcgagaa
      901 gattttcaag gtggtgtacc ctgttgatga tcaccacttc aaggtgatcc tccactacgg
      961 aactctcgtg atcgatggtg tgaccccaaa catgatcgat tacttcggaa ggccatacga
     1021 gggaatcgct gtgttcgatg gaaagaagat taccgttacc ggaaccctct ggaacggaaa
     1081 caagattatc gatgagaggc tcatcaaccc tgatggatct ctcttgttca gggtgaccat
     1141 caacggtgtg actggttgga gactttgcga gagaatcctt gctaccggct ggtcccaccc
     1201 ccagttcgag aagtaatact agagacgggt ggcatccctg tgacccctcc ccagtgcctc
     1261 tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt
     1321 gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat
     1381 ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa
     1441 gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga
     1501 ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct
     1561 aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact
     1621 cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa
     1681 ccactgctcc cttccctgtc cttt
//