LOCUS       BBa_K2170052            3880 bp    DNA     linear       22-Nov-2024
DEFINITION  Secretory prokaryotic biotin binding receptor with single chain 
            avidin
ACCESSION   BBa_K2170052
VERSION     BBa_K2170052.1  
FEATURES             Location/Qualifiers
     misc_feature    1..883
                     /label=TetR-LVA
     misc_feature    936..989
                     /label=TetO
     CDS             1038..1100
                     /label=OmpA
     CDS             1107..2777
                     /label=scAvidin
     misc_feature    2784..2816
                     /label=A3C5 Tag
     CDS             2823..3734
                     /label=EspP
     misc_feature    3752..3880
                     /label=double terminator
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gacccgacac
      901 catcgaatgg ccagatgatt aattcctaat ttttgttgac actctatcat tgatagagtt
      961 attttaccac tccctatcag tgatagagaa aagtgaaatg aatagttcga caaaaatcga
     1021 gataacgagg gcaaaaaatg aaaaagacag ctatcgcgat tgcagtggca ctggctggtt
     1081 tcgctaccgt agcgcaggcc accggcgcta gaaaaagaac ccagcctacc ttcggcttca
     1141 ccgtgaactg gaagttcagc gagagcacca ccgtgttcac cggccagtgc ttcatcgaca
     1201 gaaacggcaa agaggtgctg aaaaccatgt ggctgctgag aagcagcgtg aacgacatcg
     1261 gcgacgactg gaaggccacc agagtgggca tcaacatctt caccagactg aggacccaga
     1321 aagagggcgg ctctggcgga agcgccagaa agtgtagcct gaccggcaag tggaccaacg
     1381 acctgggcag caacatgacc atcggcgccg tgaacagcag aggcgagttc acaggcacct
     1441 acatcaccgc cgtgaccgcc accagcaacg agatcaaaga gagccccctg cacggcaccc
     1501 agaacaccat caacaagagc ggcggcagca caacagtgtt tacaggacag tgttttatcg
     1561 accggaatgg gaaagaagtg ctgaaaacaa tgtggctgct gcggtcctcc gtgaacgaca
     1621 ttggagatga ttggaaagct acacgagtgg ggattaacat ttttacccgg ctgcgcacac
     1681 agaaagaagg gggcagcggc ggctccgcta gaaagtgttc tctgactgga aaatggacaa
     1741 acgatctggg gtccaatatg acaatcgggg cagtgaactc taggggcgag tttaccggaa
     1801 catatattac agccgtgaca gctacctcta acgaaatcaa agagtctcct ctgcacggga
     1861 cacagaatac cattaacaaa agaacccagc ccacattcgg gtttacagtg aattggaaat
     1921 tctccgaggg cggcagcgga agcggatctg gctctggatc tggcaggaca cagcccacct
     1981 ttggattcac tgtgaattgg aagttttctg agtctaccac agtgttcact gggcagtgtt
     2041 tcattgatcg caatggaaaa gaggtgctga aaactatgtg gctgctgcgc tcaagtgtga
     2101 atgacatcgg ggatgattgg aaggcaactc gcgtgggaat caatatcttt acacggctga
     2161 gaactcagaa agagggggga agcggaggca gcgcccggaa atgctctctg acagggaagt
     2221 ggactaatga tctgggctct aacatgacta ttggagctgt gaatagccgg ggagagttca
     2281 ccgggactta tatcactgct gtgactgcca cctcaaatga gatcaaagaa tcccccctgc
     2341 atggaacaca gaacactatt aacaagtccg gcggctccac aaccgtgttc acagggcagt
     2401 gctttattga ccggaacggc aaagaggtgc tgaaaacaat gtggctgctg cgaagctctg
     2461 tgaatgatat tggggacgac tggaaagcaa ctagagtggg gatcaatatt ttcactcgcc
     2521 tgcggaccca gaaagaaggc ggaagcggag gatctgccag aaagtgctca ctgacaggca
     2581 aatggacaaa tgacctgggg agtaatatga ctattggggc cgtgaacagt cgcggcgagt
     2641 ttactgggac ttacattacc gcagtgacag caacatccaa tgagatcaaa gaaagtcctc
     2701 tgcatggcac tcagaacaca atcaacaaaa ggacccagcc aacctttggc tttaccgtga
     2761 attggaagtt ctctgaaacc ggcttcgaca aaaactacgt agctaactct ggtaagaccg
     2821 gcgccccagc aaacaaggaa gcaacccgga atgccgccgc cctgttctct gttgactata
     2881 aagcgtttct gaacgaggtc aacaacctga acaaacgtat gggtgacctg cgtgatatca
     2941 acggcgaagc cggtgcatgg gcacgcatca tgagcggtac cggctctgcc agtggtggtt
     3001 tcagtgacaa ctacacgcac gttcaggtcg gggtcgacaa aaaacacgag ctggacggac
     3061 tggatttgtt taccggcttc actgtcacac acactgacag cagtgcctcc gccgatgttt
     3121 tcagtggtaa aacgaagtct gtgggggctg gcctgtatgc ttccgccatg tttgattccg
     3181 gtgcctatat caacctgatt ggcaagtatg ttcaccatga taatgagtac actgcaacct
     3241 ttgccggact cggaacccgt gattacagca cgcattcatg gtatgccggt gcagaagcgg
     3301 gctaccgcta tcatgtcact gaggatgcct ggattgagcc acaggctgag ctggtttacg
     3361 gttctgtatc cggtaaacag tttgcatgga aggaccaggg aatgcatctg tccatgaagg
     3421 acaaggacta caatccgctg attggccgaa cgggtgtgga tgtgggtaaa tccttctctg
     3481 gtaaggactg gaaagtgaca gcccgtgccg gtctgggcta ccagttcgac ctgctggcta
     3541 acggcgaaac cgtattgcgg gatgcatctg gtgaaaaacg catcaaaggt gaaaaggaca
     3601 gccgtatgct gatgtccgtt ggcctgaatg cagaaatcag ggataacgtc cgctttggac
     3661 tggagtttga gaaatccgcc tttggtaagt acaacgttga taatgctgtc aacgctaatt
     3721 tccgttactc gttcaccggt taatactaga gccaggcatc aaataaaacg aaaggctcag
     3781 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     3841 cacactggct caccttcggg tgggcctttc tgcgtttata
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