LOCUS       BBa_K2170053            1786 bp    DNA     linear       23-Dec-2024
DEFINITION  Secretory NanoLuc with StrepTag (TetR_TetO_OmpA_NanoLuc_StrepTag_do
            uble terminator)
ACCESSION   BBa_K2170053
VERSION     BBa_K2170053.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=constitutiv (BBa_J23105)
     RBS             44..55
                     /label=RBS (BBa_B0034)
     CDS             62..746
                     /label=TetR
     misc_feature    755..883
                     /label=double terminator (B0015)
     misc_feature    936..989
                     /label=TetO
     CDS             1038..1100
                     /label=OmpA
     CDS             1107..1616
                     /label=NanoLuc
     misc_feature    1623..1646
                     /label=Strep TagII
     misc_feature    1658..1786
                     /label=double terminator (B0015)
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gacccgacac
      901 catcgaatgg ccagatgatt aattcctaat ttttgttgac actctatcat tgatagagtt
      961 attttaccac tccctatcag tgatagagaa aagtgaaatg aatagttcga caaaaatcga
     1021 gataacgagg gcaaaaaatg aaaaagacag ctatcgcgat tgcagtggca ctggctggtt
     1081 tcgctaccgt agcgcaggcc accggcgtgt tcaccctcga ggatttcgtt ggagattgga
     1141 gacagaccgc tggatacaac cttgatcagg ttttggagca gggtggtgtg tctagtcttt
     1201 tccagaacct cggagtgtct gtgaccccta tccagagaat cgttctctct ggtgagaacg
     1261 gactcaagat cgatatccac gtgatcatcc cttacgaggg actctcaggt gatcagatgg
     1321 gacagatcga gaagattttc aaggtggtgt accctgttga tgatcaccac ttcaaggtga
     1381 tcctccacta cggaactctc gtgatcgatg gtgtgacccc aaacatgatc gattacttcg
     1441 gaaggccata cgagggaatc gctgtgttcg atggaaagaa gattaccgtt accggaaccc
     1501 tctggaacgg aaacaagatt atcgatgaga ggctcatcaa ccctgatgga tctctcttgt
     1561 tcagggtgac catcaacggt gtgactggtt ggagactttg cgagagaatc cttgctaccg
     1621 gctggtccca cccccagttc gagaagtaat actagagcca ggcatcaaat aaaacgaaag
     1681 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1741 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//