LOCUS BBa_K2170142 680 bp DNA linear 22-Nov-2024 DEFINITION Intermediate of biotin binding receptor (NanoLuc_StrepTag_double terminator) in RFC[25] C-part ACCESSION BBa_K2170142 VERSION BBa_K2170142.1 FEATURES Location/Qualifiers CDS 1..510 /label=NanoLuc Luciferase misc_feature 1..510 /label=BBa_K1159001 misc_feature 519..542 /label=BBa_K2170202 misc_feature 551..630 /label=BBa_B0010 stem_loop 562..605 /label=stem_loop misc_feature 639..679 /label=BBa_B0012 stem_loop 646..665 /label=T7 TE polyA_site 666..679 /label=polya misc_feature 672..672 /label=stop misc_feature 551..679 /label=BBa_B0015 ORIGIN 1 gtgttcaccc tcgaggattt cgttggagat tggagacaga ccgctggata caaccttgat 61 caggttttgg agcagggtgg tgtgtctagt cttttccaga acctcggagt gtctgtgacc 121 cctatccaga gaatcgttct ctctggtgag aacggactca agatcgatat ccacgtgatc 181 atcccttacg agggactctc aggtgatcag atgggacaga tcgagaagat tttcaaggtg 241 gtgtaccctg ttgatgatca ccacttcaag gtgatcctcc actacggaac tctcgtgatc 301 gatggtgtga ccccaaacat gatcgattac ttcggaaggc catacgaggg aatcgctgtg 361 ttcgatggaa agaagattac cgttaccgga accctctgga acggaaacaa gattatcgat 421 gagaggctca tcaaccctga tggatctctc ttgttcaggg tgaccatcaa cggtgtgact 481 ggttggagac tttgcgagag aatccttgct accggctggt cccaccccca gttcgagaag 541 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 601 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 661 tgggcctttc tgcgtttata //