LOCUS       BBa_K2170211            1460 bp    DNA     linear       22-Nov-2024
DEFINITION  Secretory biotin ligase BirA (IRES_Kozak_IgKappa_BirA_StrepDEL) in 
            RFC[10]
ACCESSION   BBa_K2170211
VERSION     BBa_K2170211.1  
FEATURES             Location/Qualifiers
     RBS             35..374
                     /label=IRES
     CDS             390..449
                     /label=IgKappa
     CDS             459..1418
                     /label=BirA
     misc_feature    1425..1457
                     /label=misc
ORIGIN
        1 tagtgatcag cctcgactgt gccttcacgt agtgagatgg gggtcctggg ccccagggtg
       61 tgcagccact gacttgggga ctgctggtgg ggtagggatg agggagggag gggcattgtg
      121 atgtacaggg ctgctctgtg agatcaaggg tctcttaagg gtgggagctg gggcagggac
      181 tacgagagca gccagatggg ctgaaagtgg aactcaaggg gtttctggca cctacctacc
      241 tgcttcccgc tggggggtgg ggagttggcc cagagtctta agattggggc agggtggaga
      301 ggtgggctct tcctgcttcc cactcatctt atagctttct ttccccagat ccgaattgga
      361 gatccaaacc aagggatcca gccgccacca tggaaaccga caccctgctg ctgtgggtgc
      421 tgctgctgtg ggtgccagga tctacaggcg acggcgccaa ggacaacacc gtgcccctga
      481 agctgattgc cctgctggcc aacggcgagt tccactctgg cgagcagctg ggcgagacac
      541 tgggcatgtc ccgtgccgcc atcaacaagc acatccagac cctgcgtgac tggggcgtgg
      601 acgtgttcac cgtgcctggc aagggctaca gcctgcccga gcctatccag ctgctgaacg
      661 ccaagcagat cctgggccag ctggatggcg gatctgtggc tgtgctgccc gtgatcgaca
      721 gcaccaacca gtacctgctg gaccggatcg gcgagctgaa aagcggcgac gcctgtatcg
      781 ccgagtatca gcaggctggc cgtggccgtc ggggccgtaa gtggtttagc cccttcggcg
      841 caaatctgta cctgagcatg ttctggcggc tggaacaggg acctgccgcc gctattggac
      901 tgagcctcgt gatcggcatc gtgatggccg aggtgctgcg taagctgggt gccgacaaag
      961 tgcgcgtgaa gtggcccaac gacctgtatc tgcaagaccg taagctggca ggcatcctgg
     1021 tggaactgac aggcaaaact ggcgacgccg cccagatcgt gattggcgct ggcatcaata
     1081 tggccatgcg tcgggtggaa gagtccgtcg tgaatcaggg ctggatcacc ctgcaagaag
     1141 ccggtatcaa cctggaccgt aacaccctgg ccgccatgct gatccgtgag ctgcgtgccg
     1201 ccctggaact gttcgagcag gaaggcctgg ccccctacct gtcccgttgg gagaagctgg
     1261 acaacttcat caaccgtccc gtgaaactga tcatcggcga caaagagatc ttcggcatca
     1321 gccggggcat cgacaagcag ggcgctctgc tgctggaaca ggacggcatc atcaagccct
     1381 ggatgggcgg cgagatcagc ctgcgttctg ccgagaaagg tgcctggtcc cacccccagt
     1441 tcgagaagga tgagctgtaa
//