LOCUS       BBa_K2171001            2282 bp    DNA     linear       23-Dec-2024
DEFINITION  RvLEAM - osmotic stress protein from tardigrade
ACCESSION   BBa_K2171001
VERSION     BBa_K2171001.1  
FEATURES             Location/Qualifiers
     CDS             1..1083
                     /label=LacI
     promoter        1162..1190
                     /label=tac promoter
     misc_feature    1195..1219
                     /label=lac operator
     CDS             1267..2148
                     /label=RvLEAM
     misc_feature    2250..2277
                     /label=rrnbT1 terminator
     misc_feature    2012..2039
                     /label=T7Te terminator
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
       61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
      301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
      901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
      961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt
     1201 gtgagcggat aacaattccc tcatgaaata attttgtttt actagagtca cacaggaaag
     1261 tactagatgt ttttagctcg taatgctggc cgtgcaggct atcgcggtgt agtggcgtat
     1321 cagcaagccg cttctttcag cgtgagttcc gcaaaagctg ccggatcacg ctcgtctggg
     1381 gggagtgacg cgggagacta tgcccgcgag gccgcggagc acgctaaggc aggattgaag
     1441 gaccttaaaa acgaggcgtc ttggaaagct aaaggagttg cgaaccaagc cgccggtgct
     1501 tttgagcgtg ctaaagatac tgtgaaggag ggggtccacg acatgaaacg ctcaggtagt
     1561 cgcgttttcg aacaaggaca agaggaagtg gaagccggtg ctcagcacgc taaggcaggc
     1621 taccaaagcg ccaaaaatgc ggcacaggat acggctgcta cattgaagga caaggcgggg
     1681 tccgcttgga accaggccaa acacgttgtg gaagacaagg gtgaggacgt tgtcgaagct
     1741 gttaaagaca ccgcaagtaa ggtctgggga aaagccaaac acgttgctga ggacgtaaag
     1801 gagaatgcgc aatcccccgg cggaatcgct gacaaagcct cggatgtatg gtccgcagct
     1861 aaggataaag ctgcggacgt gctgagcggc gctaagcaca cggccgagaa cttggcgcac
     1921 aaggcgcaag ccgctatcca cgatgcaacg gcatctagcg ggtcccagtc acagtctcaa
     1981 tcccaatcac aatatcgcca gggccaacag caaggtcgtc aagatcaaca acaaagcaaa
     2041 agccaatggg gacagacctc accccagtcg cctgacgggt ttcgtccaca agctggtcaa
     2101 ggtccccagg gcgggaaagg gccgggacag gccggaggcc gtcgttaata attgattaac
     2161 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     2221 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     2281 at
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