LOCUS BBa_K2171001 2282 bp DNA linear 23-Nov-2024 DEFINITION RvLEAM - osmotic stress protein from tardigrade ACCESSION BBa_K2171001 VERSION BBa_K2171001.1 FEATURES Location/Qualifiers CDS 1..1083 /label=LacI promoter 1162..1190 /label=tac promoter misc_feature 1195..1219 /label=lac operator CDS 1267..2148 /label=RvLEAM misc_feature 2250..2277 /label=rrnbT1 terminator misc_feature 2012..2039 /label=T7Te terminator ORIGIN 1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga 121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc 181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata 241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag 301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat 361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc 421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg 481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat 541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga 601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt 661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag 721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct 781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc 841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa 901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat 961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg 1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt 1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt 1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt 1201 gtgagcggat aacaattccc tcatgaaata attttgtttt actagagtca cacaggaaag 1261 tactagatgt ttttagctcg taatgctggc cgtgcaggct atcgcggtgt agtggcgtat 1321 cagcaagccg cttctttcag cgtgagttcc gcaaaagctg ccggatcacg ctcgtctggg 1381 gggagtgacg cgggagacta tgcccgcgag gccgcggagc acgctaaggc aggattgaag 1441 gaccttaaaa acgaggcgtc ttggaaagct aaaggagttg cgaaccaagc cgccggtgct 1501 tttgagcgtg ctaaagatac tgtgaaggag ggggtccacg acatgaaacg ctcaggtagt 1561 cgcgttttcg aacaaggaca agaggaagtg gaagccggtg ctcagcacgc taaggcaggc 1621 taccaaagcg ccaaaaatgc ggcacaggat acggctgcta cattgaagga caaggcgggg 1681 tccgcttgga accaggccaa acacgttgtg gaagacaagg gtgaggacgt tgtcgaagct 1741 gttaaagaca ccgcaagtaa ggtctgggga aaagccaaac acgttgctga ggacgtaaag 1801 gagaatgcgc aatcccccgg cggaatcgct gacaaagcct cggatgtatg gtccgcagct 1861 aaggataaag ctgcggacgt gctgagcggc gctaagcaca cggccgagaa cttggcgcac 1921 aaggcgcaag ccgctatcca cgatgcaacg gcatctagcg ggtcccagtc acagtctcaa 1981 tcccaatcac aatatcgcca gggccaacag caaggtcgtc aagatcaaca acaaagcaaa 2041 agccaatggg gacagacctc accccagtcg cctgacgggt ttcgtccaca agctggtcaa 2101 ggtccccagg gcgggaaagg gccgggacag gccggaggcc gtcgttaata attgattaac 2161 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 2221 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 2281 at //