LOCUS       BBa_K2171002            2024 bp    DNA     linear       29-Nov-2024
DEFINITION  MASH - osmotic stress protein from tardigrade
ACCESSION   BBa_K2171002
VERSION     BBa_K2171002.1  
FEATURES             Location/Qualifiers
     promoter        1162..1190
                     /label=tac promoter
     misc_feature    1195..1219
                     /label=lac operator
     CDS             1..1083
                     /label=LacI
     CDS             1267..1890
                     /label=MASH
     misc_feature    1905..1976
                     /label=rrnbT1 terminator
     misc_feature    1992..2019
                     /label=T7Te terminator
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
       61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
      301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
      901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
      961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt
     1201 gtgagcggat aacaattccc tcatgaaata attttgtttt actagagtca cacaggaaag
     1261 tactagatgg tttcgttgcg tttaggtgat ttccgctcgc agccaagttt acgtagcgtg
     1321 ccagcgagct taacttcccg cagtcaggcg ttcagccttc aggagattgc agcgcgcgcg
     1381 ggggtagtac tgcgtggcgt gcaacaacag ttccgtaacg taacgggagt gaatgctgcc
     1441 ccggtggtgg cctttgacaa cggctcagtc ctttattctg agcgcatcca tagccaatca
     1501 tctcaaaagc aggcccccac aacagtcccc acaggcagcg tcagcaatag tcctcaaccg
     1561 gaggggaaag ccaacgaagc cgccgagcgt gccaaacaat ttatgaaccc acccgtagcc
     1621 ccaatggacc cagtcgacaa aaatgagttc gtggctatgc ccgagatggg gcgctccaac
     1681 ggaaatggcg agaacaagca ggcagcggac tttatgaaga atcagggcga tactgacatg
     1741 gactcacagt acgccccaga ctccagtaag aacacgaagt ctgtcccgac gaaggaaatc
     1801 gtggcagagg acggaagtat gtctattgag gatattaaaa aagcgacgca ggttactcca
     1861 ggggtcgcgg ttaaaaatga gggggtataa taattgatta acatcaaata aaacgaaagg
     1921 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1981 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttat
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