LOCUS       BBa_K2171003            1922 bp    DNA     linear       26-Nov-2024
DEFINITION  SAHS2 - osmotic stress protein from tardigrade with periplasmic 
            secretion signal peptide
ACCESSION   BBa_K2171003
VERSION     BBa_K2171003.1  
FEATURES             Location/Qualifiers
     promoter        1162..1190
                     /label=tac promoter
     misc_feature    1195..1219
                     /label=lac operator
     CDS             1..1083
                     /label=LacI
     CDS             1267..1788
                     /label=SAHS2
     misc_feature    1803..1874
                     /label=rrnB terminator
     misc_feature    1890..1917
                     /label=T7Te terminator
     misc_feature    1290..1340
                     /label=Secretion signal peptide
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
       61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
      301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
      901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
      961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt
     1201 gtgagcggat aacaattccc tcatgaaata attttgtttt actagagtca cacaggaaag
     1261 tactagatga aaaaactgct gttcgcgatt ccgctggtgg tgccgttcta tagccatagc
     1321 gcagatgacg cagctcacga ggaaggggtg gagtggacgg gtaagccgtg gatgggaaaa
     1381 tgggagtcag atccttctaa ggatgagaat gtagaagagt ttaagaaaaa attgcagtta
     1441 cctatgtccc attcggaaat gaacaagaac tccaaagtct ggattcacca ctataaaaaa
     1501 ggcgacgaat accatcataa gattattatc aatgacgcgc attacaaaaa tgatatcgtg
     1561 ttcaaattgg gacaagagag tgccgggtcg tataatggct catcattctc cgtgaaatac
     1621 gaagacaaag acggtgcttt agttggaagc gtacactaca cgggtacaaa agagcaaagt
     1681 cttgataaaa ccatcaataa tgtattcaag ctggagggcg accacctggt gaagacgtcc
     1741 accattgagg gggttaccat gaagcgccac tacaacaaac gtcagtaata attgattaac
     1801 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1861 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1921 at
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