LOCUS BBa_K2172012 4254 bp DNA linear 29-Nov-2024 DEFINITION Tac Promoter-RBS-GST-Thrombin Protease-SmCPS1-TEV-GFP-Terminator ACCESSION BBa_K2172012 VERSION BBa_K2172012.1 FEATURES Location/Qualifiers ORIGIN 1 tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac aatttcacac 61 aggaaacagt attcatgtcc cctatactag gttattggaa aattaagggc cttgtgcaac 121 ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg tatgagcgcg 181 atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt cccaatcttc 241 cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata cgttatatag 301 ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg 361 aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt aaagactttg 421 aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc 481 gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt 541 atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg ttcccaaaat 601 tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac ttgaaatcca 661 gcaagtatat agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc 721 ctccaaaatc ggatctggtt ccgcgtggat ccccgggaat ggcctcctta tcctctacaa 781 tcctcagccg ctctccggcg gcccgccgca gaattacgcc ggcatcggct aagcttcacc 841 tgccggaatg tttcgccatc agtgcatgga tgggcagcag cactaaaaac ctttctctca 901 gctaccaact taatcacaag aaaatatcag ttgccacagt agatgcgccg caggtgcatg 961 accacgacgg cactaccgtt catcaaggcc atgatgcggt gaagaatatt gaggatccca 1021 ttgaatacat caggacgttg ttgaggacga cgggggacgg gagaataagc gtgtcgccgt 1081 acgacacggc gtgggtggcg atgatcaagg acgtggaggg gcgggacggg ccccagttcc 1141 cctccagcct cgagtggatc gtgcagaatc aactcgagga tggatcgtgg ggcgatcaga 1201 agcttttctg cgtctacgat cgcctcgtca ataccatcgc gtgcgtggta gccttgagat 1261 cgtggaatgt tcatgctcac aaggtcaaaa gaggagtgac gtacatcaag gaaaatgtgg 1321 ataaacttat ggagggaaat gaggagcaca tgacttgtgg gttcgaagtg gtgtttccgg 1381 cgcttctaca aaaagcgaaa agcttaggca tcgaagatct tccttacgat tctccggcgg 1441 tgcaggaggt ttatcatgtc agggaacaaa agttgaaaag gattccactg gagattatgc 1501 acaaaatacc gacatcatta ttatttagtt tggaagggct cgaaaatttg gattgggaca 1561 aacttttgaa actccagtca gccgacggtt ccttcctcac ctctccctcc tccaccgcct 1621 tcgcgttcat gcaaaccaag gatgaaaaat gctaccaatt catcaagaac acgatagaca 1681 ctttcaacgg aggagcgcca cacacttatc ccgtcgacgt gtttggaagg ctctgggcga 1741 tcgaccggct ccagcgcctc ggaatttccc gcttttttga gccggagatt gctgattgct 1801 taagccacat ccacaaattt tggacggata agggagtttt cagtgggaga gaatcggagt 1861 tttgcgacat tgacgataca tccatgggaa tgaggcttat gaggatgcat ggatatgatg 1921 ttgatccaaa tgtgctgagg aatttcaagc agaaagatgg taaattctct tgctacggcg 1981 ggcagatgat cgagtcgcct tctccgatat acaatcttta cagagcttct cagctccgat 2041 ttcccggcga ggaaatcctc gaagatgcga agaaattcgc ctacgatttc ttgaaagaaa 2101 aactagccaa caatcagatt ctggataaat gggttatttc taagcacttg cctgatgaga 2161 tcaagctcgg gctagagatg ccgtggctcg ccaccctacc ccgcgtcgag gcgaagtact 2221 acatccagta ctacgccggc tccggcgacg tgtggatcgg gaagacgctg tacaggatgc 2281 cggagatcag caacgacacg taccacgacc tagccaagac ggatttcaag agatgccaag 2341 cgaagcatca gttcgagtgg ctctacatgc aagaatggta cgagagctgc ggcatcgagg 2401 agttcgggat aagcagaaag gaccttctgc tttcctattt cttggcgacc gcgagcatct 2461 tcgagctcga gaggaccaac gagcgaatcg cgtgggccaa atcgcagatc atcgctaaga 2521 tgatcacttc tttcttcaac aaggaaacta cgtcggagga ggacaagcga gctcttttga 2581 acgagctcgg aaacattaat ggcctcaacg acacaaacgg cgcagggaga gaaggtgggg 2641 ccggtagcat tgcgctagcg accctcactc agttcctcga gggattcgac agatacacca 2701 gacaccagct gaaaaatgct tggagcgtat ggctgacgca gctgcaacat ggcgaagcag 2761 acgacgcaga gctcctaacc aacacgttga acatctgcgc cggccacatc gccttcaggg 2821 aagaaatact ggcgcacaac gagtacaaaa ctctctccaa cctaaccagc aaaatctgtc 2881 ggcaactttc tttcattcaa agcgaaaagg agatgggagt agagggcgag atcgcagcga 2941 aatcgagcat aaaaaacaag gaactcgaag aagacatgca aatgttggtg aagttggtgc 3001 ttgagaaata tgggggcata gatagaaata taaagaaagc gtttttggca gttgcgaaga 3061 cttattatta cagagcgtat catgccgccg acaccataga cacacacatg tttaaagtgc 3121 ttttcgagcc agtcgcgtga cctagactcc atgggtcgac tcgaaaacct gtattttcag 3181 ggcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 3241 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 3301 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 3361 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg 3421 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 3481 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 3541 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 3601 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 3661 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 3721 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 3781 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 3841 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 3901 taaaagctta ttcatcgtga ctgactgacg atctgcctcg cgcgtttcgg tgatgacggt 3961 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 4021 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 4081 atgacccagt cacgtagcga tagcggagtg tataattctt gaagacgaaa gggcctcgtg 4141 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 4201 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acat //