##gff-version 3
##sequence-region BBa_K2173000 1 2973
BBa_K2173000	.	promoter	1	68	.	+	0	ID=BBa_K415032;Name=BBa_K415032
BBa_K2173000	.	start_codon	51	51	.	+	0	ID=annotation2169677;Name=Transcription Start Site;Parent=BBa_K415032
BBa_K2173000	.	non_covalent_binding_site	42	47	.	+	0	ID=annotation2169675;Name=Pseudo-Pribnow Box;Parent=BBa_K415032
BBa_K2173000	.	operator	1	21	.	+	0	ID=annotation2169674;Name=LuxR-AHL Binding Box;Parent=BBa_K415032
BBa_K2173000	.	operator	52	68	.	+	0	ID=annotation2169676;Name=Lambda Repressor OR1;Parent=BBa_K415032
BBa_K2173000	.	ribosome_entry_site	2048	2059	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2173000	.	conserved_region	2052	2055	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2173000	.	promoter	1057	1124	.	+	0	ID=BBa_K415032;Name=BBa_K415032
BBa_K2173000	.	start_codon	1107	1107	.	+	0	ID=annotation2169677;Name=Transcription Start Site;Parent=BBa_K415032
BBa_K2173000	.	non_covalent_binding_site	1098	1103	.	+	0	ID=annotation2169675;Name=Pseudo-Pribnow Box;Parent=BBa_K415032
BBa_K2173000	.	operator	1057	1077	.	+	0	ID=annotation2169674;Name=LuxR-AHL Binding Box;Parent=BBa_K415032
BBa_K2173000	.	operator	1108	1124	.	+	0	ID=annotation2169676;Name=Lambda Repressor OR1;Parent=BBa_K415032
BBa_K2173000	.	terminator	1868	1996	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2173000	.	terminator	1956	1996	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2173000	.	polyA_site	1983	1996	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2173000	.	engineered_region	1956	1996	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2173000	.	stem_loop	1963	1982	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2173000	.	stop_codon	1989	1989	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2173000	.	terminator	1868	1947	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2173000	.	stem_loop	1879	1922	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2173000	.	engineered_region	1868	1947	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2173000	.	promoter	2005	2039	.	+	0	ID=BBa_J23114;Name=BBa_J23114
BBa_K2173000	.	terminator	920	1048	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2173000	.	terminator	1008	1048	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2173000	.	polyA_site	1035	1048	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2173000	.	engineered_region	1008	1048	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2173000	.	stem_loop	1015	1034	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2173000	.	stop_codon	1041	1041	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2173000	.	terminator	920	999	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2173000	.	stem_loop	931	974	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2173000	.	engineered_region	920	999	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2173000	.	ribosome_entry_site	1133	1147	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K2173000	.	ribosome_entry_site	1133	1147	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2173000	.	ribosome_entry_site	1140	1144	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2173000	.	engineered_region	1133	1147	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2173000	.	ribosome_entry_site	77	91	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K2173000	.	ribosome_entry_site	77	91	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2173000	.	ribosome_entry_site	84	88	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2173000	.	engineered_region	77	91	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2173000	.	CDS	1154	1859	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K2173000	.	CDS	1154	1828	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K2173000	.	engineered_tag	1835	1859	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K2173000	.	terminator	2788	2916	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2173000	.	terminator	2876	2916	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2173000	.	polyA_site	2903	2916	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2173000	.	engineered_region	2876	2916	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2173000	.	stem_loop	2883	2902	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2173000	.	stop_codon	2909	2909	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2173000	.	terminator	2788	2867	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2173000	.	stem_loop	2799	2842	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2173000	.	engineered_region	2788	2867	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2173000	.	CDS	98	886	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_K2173000	.	engineered_tag	887	911	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K2173000	.	tag	848	880	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K2173000	.	start_codon	98	100	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K2173000	.	stop_codon	881	886	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K2173000	.	CDS	98	847	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K2173000	.	engineered_region	98	886	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K2173000	.	CDS	2066	2779	.	+	0	ID=BBa_K098997;Name=BBa_K098997
BBa_K2173000	.	CDS	2066	2779	.	+	0	ID=annotation1988780;Name=cIts;Parent=BBa_K098997
BBa_K2173000	.	promoter	2925	2973	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K2173000	.	non_covalent_binding_site	2949	2965	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K2173000	.	engineered_region	2925	2973	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K2173000	.	non_covalent_binding_site	2925	2941	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K2173000	.	promoter	2939	2944	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K2173000	.	promoter	2962	2967	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
>BBa_K2173000
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatatactagagatt
aaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctg
ttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctat
tttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggtt
ttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttata
ttattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgc
tgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaa
ggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattg
agacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatt
tgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgtt
ttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacg
ctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcga
atacctctggcggtgatatactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagtt
catgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccg
tacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgc
agttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaagg
tttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggt
gagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggtt
gggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaaga
cggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaacc
gacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcact
ccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagtttatggctagctcagtcctaggtacaatgctagctactagagaaagagg
agaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaattta
tgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggt
gctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaag
aatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagta
tgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggag
agatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaa
caggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctg
catagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccacta
aacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaag
agacgtttggctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc