LOCUS BBa_K2173001 2789 bp DNA linear 25-Nov-2024 DEFINITION LuxI_LuxR_mrfp1 ACCESSION BBa_K2173001 VERSION BBa_K2173001.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 RBS 64..78 /label=RBS-1\Strong RBS 71..75 /label=RBS misc_feature 64..78 /label=BBa_B0030 RBS 64..78 /label=BBa_B0030 misc_feature 664..695 /label=LVA CDS 85..663 /label=luxI misc_feature 85..702 /label=BBa_C0061 misc_feature 703..727 /label=Help:Barcodes CDS 85..702 /label=BBa_C0061 stem_loop 747..790 /label=stem_loop misc_feature 736..815 /label=BBa_B0010 terminator 736..815 /label=BBa_B0010 stem_loop 831..850 /label=T7 TE misc_feature 857..857 /label=stop polyA_site 851..864 /label=polya misc_feature 824..864 /label=BBa_B0012 terminator 824..864 /label=BBa_B0012 terminator 736..864 /label=BBa_B0015 promoter 873..907 /label=BBa_J23100 RBS 916..930 /label=RBS-1\Strong RBS 923..927 /label=RBS misc_feature 916..930 /label=BBa_B0030 RBS 916..930 /label=BBa_B0030 misc_feature 937..938 /label=prefix misc_feature 1110..1110 /label=T misc_feature 1428..1428 /label=A CDS 937..1686 /label=luxR misc_feature 937..1692 /label=BBa_C0062 misc_feature 1693..1717 /label=Help:Barcodes CDS 937..1692 /label=BBa_C0062 stem_loop 1737..1780 /label=stem_loop misc_feature 1726..1805 /label=BBa_B0010 terminator 1726..1805 /label=BBa_B0010 stem_loop 1821..1840 /label=T7 TE misc_feature 1847..1847 /label=stop polyA_site 1841..1854 /label=polya misc_feature 1814..1854 /label=BBa_B0012 terminator 1814..1854 /label=BBa_B0012 terminator 1726..1854 /label=BBa_B0015 misc_feature 1863..1882 /label=LuxR/HSL promoter 1882..1887 /label=-35 promoter 1904..1909 /label=-10 misc_feature 1915..1915 /label=start misc_feature 1863..1917 /label=BBa_R0062 promoter 1863..1917 /label=BBa_R0062 RBS 1926..1940 /label=RBS-1\Strong RBS 1933..1937 /label=RBS misc_feature 1926..1940 /label=BBa_B0030 RBS 1926..1940 /label=BBa_B0030 CDS 1947..2621 /label=mrfp1 misc_feature 2628..2652 /label=Help:Barcodes CDS 1947..2652 /label=BBa_E1010 stem_loop 2672..2715 /label=stem_loop misc_feature 2661..2740 /label=BBa_B0010 terminator 2661..2740 /label=BBa_B0010 stem_loop 2756..2775 /label=T7 TE misc_feature 2782..2782 /label=stop polyA_site 2776..2789 /label=polya misc_feature 2749..2789 /label=BBa_B0012 terminator 2749..2789 /label=BBa_B0012 terminator 2661..2789 /label=BBa_B0015 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagattaaag aggagaaata ctagatgact ataatgataa aaaaatcgga ttttttggca 121 attccatcgg aggagtataa aggtattcta agtcttcgtt atcaagtgtt taagcaaaga 181 cttgagtggg acttagttgt agaaaataac cttgaatcag atgagtatga taactcaaat 241 gcagaatata tttatgcttg tgatgatact gaaaatgtaa gtggatgctg gcgtttatta 301 cctacaacag gtgattatat gctgaaaagt gtttttcctg aattgcttgg tcaacagagt 361 gctcccaaag atcctaatat agtcgaatta agtcgttttg ctgtaggtaa aaatagctca 421 aagataaata actctgctag tgaaattaca atgaaactat ttgaagctat atataaacac 481 gctgttagtc aaggtattac agaatatgta acagtaacat caacagcaat agagcgattt 541 ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca aagaaattca tgtattaggt 601 gatactaaat cggttgtatt gtctatgcct attaatgaac agtttaaaaa agcagtctta 661 aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt 721 agatctctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 781 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 841 cgggtgggcc tttctgcgtt tatatactag agttgacggc tagctcagtc ctaggtacag 901 tgctagctac tagagattaa agaggagaaa tactagatga aaaacataaa tgccgacgac 961 acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc 1021 ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct 1081 cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa 1141 tattatgatg acgctaattt aataaaatat gatcctatag tagattattc taactccaat 1201 cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat 1261 gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg 1321 gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat 1381 agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat 1441 tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa 1501 gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt 1561 tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca 1621 aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt 1681 aaaaattaat aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa 1741 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 1801 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 1861 agacctgtag gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac 1921 tagagattaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca 1981 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg 2041 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg 2101 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt 2161 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca 2221 aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct 2281 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg 2341 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc 2401 cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc 2461 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg 2521 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg 2581 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct 2641 agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 2701 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 2761 accttcgggt gggcctttct gcgtttata //