##gff-version 3
##sequence-region BBa_K2173001 1 2789
BBa_K2173001	.	ribosome_entry_site	64	78	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K2173001	.	ribosome_entry_site	71	75	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2173001	.	engineered_region	64	78	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2173001	.	ribosome_entry_site	64	78	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2173001	.	terminator	1726	1854	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2173001	.	terminator	1726	1805	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2173001	.	engineered_region	1726	1805	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2173001	.	stem_loop	1737	1780	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2173001	.	terminator	1814	1854	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2173001	.	stop_codon	1847	1847	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2173001	.	polyA_site	1841	1854	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2173001	.	stem_loop	1821	1840	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2173001	.	engineered_region	1814	1854	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2173001	.	ribosome_entry_site	1926	1940	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K2173001	.	ribosome_entry_site	1933	1937	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2173001	.	engineered_region	1926	1940	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2173001	.	ribosome_entry_site	1926	1940	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2173001	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K2173001	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K2173001	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K2173001	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K2173001	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K2173001	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K2173001	.	promoter	873	907	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K2173001	.	CDS	937	1692	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K2173001	.	engineered_tag	1693	1717	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K2173001	.	engineered_region	937	1692	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K2173001	.	engineered_region	937	938	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K2173001	.	point_mutation	1110	1110	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K2173001	.	CDS	937	1686	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K2173001	.	point_mutation	1428	1428	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K2173001	.	terminator	2661	2789	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2173001	.	terminator	2661	2740	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2173001	.	engineered_region	2661	2740	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2173001	.	stem_loop	2672	2715	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2173001	.	terminator	2749	2789	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2173001	.	stop_codon	2782	2782	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2173001	.	polyA_site	2776	2789	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2173001	.	stem_loop	2756	2775	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2173001	.	engineered_region	2749	2789	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2173001	.	terminator	736	864	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2173001	.	terminator	736	815	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2173001	.	engineered_region	736	815	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2173001	.	stem_loop	747	790	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2173001	.	terminator	824	864	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2173001	.	stop_codon	857	857	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2173001	.	polyA_site	851	864	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2173001	.	stem_loop	831	850	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2173001	.	engineered_region	824	864	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2173001	.	CDS	1947	2652	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K2173001	.	engineered_tag	2628	2652	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K2173001	.	CDS	1947	2621	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K2173001	.	CDS	85	702	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K2173001	.	CDS	85	663	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K2173001	.	engineered_tag	703	727	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K2173001	.	engineered_region	85	702	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K2173001	.	tag	664	695	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K2173001	.	promoter	1863	1917	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K2173001	.	start_codon	1915	1915	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K2173001	.	promoter	1904	1909	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K2173001	.	promoter	1882	1887	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K2173001	.	engineered_region	1863	1917	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K2173001	.	non_covalent_binding_site	1863	1882	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K2173001	.	ribosome_entry_site	916	930	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K2173001	.	ribosome_entry_site	923	927	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2173001	.	engineered_region	916	930	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2173001	.	ribosome_entry_site	916	930	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_K2173001
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaat
actagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcg
ttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactca
aatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgatt
atatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcg
ttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatat
aaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtatta
aagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctat
taatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatag
tgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatacta
gagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaaaaacata
aatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctg
atatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttc
aatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagat
tattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaa
ttaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgct
tagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgtt
ccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaag
aatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcac
tttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacagga
gcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaat
ggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaaga
gttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgt
ccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccc
cgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccgga
aggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagac
ggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgg
gttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaa
agacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaa
accgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtc
actccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttata