##gff-version 3
##sequence-region BBa_K2173002 1 2802
BBa_K2173002	.	CDS	937	1692	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K2173002	.	engineered_region	937	938	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K2173002	.	engineered_tag	1693	1717	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K2173002	.	point_mutation	1428	1428	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K2173002	.	engineered_region	937	1692	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K2173002	.	point_mutation	1110	1110	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K2173002	.	CDS	937	1686	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K2173002	.	terminator	736	864	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2173002	.	terminator	736	815	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2173002	.	stem_loop	747	790	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2173002	.	engineered_region	736	815	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2173002	.	terminator	824	864	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2173002	.	stem_loop	831	850	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2173002	.	stop_codon	857	857	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2173002	.	polyA_site	851	864	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2173002	.	engineered_region	824	864	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2173002	.	ribosome_entry_site	916	930	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K2173002	.	ribosome_entry_site	916	930	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2173002	.	engineered_region	916	930	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2173002	.	ribosome_entry_site	923	927	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2173002	.	promoter	873	907	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K2173002	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K2173002	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K2173002	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K2173002	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K2173002	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K2173002	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K2173002	.	terminator	1726	1854	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2173002	.	terminator	1726	1805	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2173002	.	stem_loop	1737	1780	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2173002	.	engineered_region	1726	1805	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2173002	.	terminator	1814	1854	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2173002	.	stem_loop	1821	1840	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2173002	.	stop_codon	1847	1847	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2173002	.	polyA_site	1841	1854	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2173002	.	engineered_region	1814	1854	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2173002	.	CDS	85	702	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K2173002	.	engineered_region	85	702	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K2173002	.	tag	664	695	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K2173002	.	CDS	85	663	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K2173002	.	engineered_tag	703	727	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K2173002	.	terminator	2674	2802	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2173002	.	terminator	2674	2753	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2173002	.	stem_loop	2685	2728	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2173002	.	engineered_region	2674	2753	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2173002	.	terminator	2762	2802	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2173002	.	stem_loop	2769	2788	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2173002	.	stop_codon	2795	2795	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2173002	.	polyA_site	2789	2802	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2173002	.	engineered_region	2762	2802	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2173002	.	CDS	1960	2665	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K2173002	.	engineered_tag	2641	2665	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K2173002	.	CDS	1960	2634	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K2173002	.	ribosome_entry_site	64	78	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K2173002	.	ribosome_entry_site	64	78	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2173002	.	engineered_region	64	78	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2173002	.	ribosome_entry_site	71	75	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2173002	.	promoter	1863	1930	.	+	0	ID=BBa_K415032;Name=BBa_K415032
BBa_K2173002	.	start_codon	1913	1913	.	+	0	ID=annotation2169677;Name=Transcription Start Site;Parent=BBa_K415032
BBa_K2173002	.	non_covalent_binding_site	1904	1909	.	+	0	ID=annotation2169675;Name=Pseudo-Pribnow Box;Parent=BBa_K415032
BBa_K2173002	.	operator	1914	1930	.	+	0	ID=annotation2169676;Name=Lambda Repressor OR1;Parent=BBa_K415032
BBa_K2173002	.	operator	1863	1883	.	+	0	ID=annotation2169674;Name=LuxR-AHL Binding Box;Parent=BBa_K415032
BBa_K2173002	.	ribosome_entry_site	1939	1953	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K2173002	.	ribosome_entry_site	1939	1953	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2173002	.	engineered_region	1939	1953	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2173002	.	ribosome_entry_site	1946	1950	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
>BBa_K2173002
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaat
actagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcg
ttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactca
aatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgatt
atatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcg
ttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatat
aaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtatta
aagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctat
taatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatag
tgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatacta
gagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaaaaacata
aatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctg
atatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttc
aatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagat
tattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaa
ttaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgct
tagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgtt
ccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaag
aatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcac
tttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacagga
gcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaat
ggtttgttatagtcgaatacctctggcggtgatatactagagattaaagaggagaaatactagatggcttcctccgaag
acgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtga
aggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgg
gacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaac
tgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactc
ctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcag
aaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgc
gtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgcc
gggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgt
gctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttata