LOCUS BBa_K2173003 2025 bp DNA linear 29-Nov-2024 DEFINITION aiiA_lambda-CI ACCESSION BBa_K2173003 VERSION BBa_K2173003.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=LuxR-AHL Binding Box misc_feature 42..47 /label=Pseudo-Pribnow Box misc_feature 52..68 /label=Lambda Repressor OR1 misc_feature 51..51 /label=Transcription Start Site promoter 1..68 /label=BBa_K415032 RBS 77..91 /label=RBS-1\Strong RBS 84..88 /label=RBS misc_feature 77..91 /label=BBa_B0030 RBS 77..91 /label=BBa_B0030 misc_feature 98..100 /label=start misc_feature 881..886 /label=2 misc_feature 848..880 /label=LVA CDS 98..847 /label=aiiA misc_feature 98..886 /label=BBa_C0060 misc_feature 887..911 /label=Help:Barcodes CDS 98..886 /label=BBa_C0060 stem_loop 931..974 /label=stem_loop misc_feature 920..999 /label=BBa_B0010 terminator 920..999 /label=BBa_B0010 stem_loop 1015..1034 /label=T7 TE misc_feature 1041..1041 /label=stop polyA_site 1035..1048 /label=polya misc_feature 1008..1048 /label=BBa_B0012 terminator 1008..1048 /label=BBa_B0012 terminator 920..1048 /label=BBa_B0015 promoter 1057..1091 /label=BBa_J23114 misc_feature 1104..1107 /label=conserved RBS 1100..1111 /label=BBa_B0034 CDS 1118..1831 /label=cIts CDS 1118..1831 /label=BBa_K098997 stem_loop 1851..1894 /label=stem_loop misc_feature 1840..1919 /label=BBa_B0010 terminator 1840..1919 /label=BBa_B0010 stem_loop 1935..1954 /label=T7 TE misc_feature 1961..1961 /label=stop polyA_site 1955..1968 /label=polya misc_feature 1928..1968 /label=BBa_B0012 terminator 1928..1968 /label=BBa_B0012 terminator 1840..1968 /label=BBa_B0015 promoter 2014..2019 /label=-10 promoter 1991..1996 /label=-35 misc_feature 2001..2017 /label=OR1 misc_feature 1977..1993 /label=OR2 misc_feature 1977..2025 /label=BBa_R0051 promoter 1977..2025 /label=BBa_R0051 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atacctctgg 61 cggtgatata ctagagatta aagaggagaa atactagatg acagtaaaga agctttattt 121 cgtcccagca ggtcgttgta tgttggatca ttcgtctgtt aatagtacat taacaccagg 181 agaattatta gacttaccgg tttggtgtta tcttttggag actgaagaag gacctatttt 241 agtagataca ggtatgccag aaagtgcagt taataatgaa ggtcttttta acggtacatt 301 tgtcgaaggg caggttttac cgaaaatgac tgaagaagat agaatcgtga atattttaaa 361 acgggttggt tatgagccgg aagaccttct ttatattatt agttctcact tgcattttga 421 tcatgcagga ggaaatggcg cttttataaa tacaccaatc attgtacagc gtgctgaata 481 tgaggcggcg cagcatagcg aagaatattt gaaagaatgt atattgccga atttaaacta 541 caaaatcatt gaaggtgatt atgaagtcgt accaggagtt caattattgc atacaccagg 601 ccatactcca gggcatcaat cgctattaat tgagacagaa aaatccggtc ctgtattatt 661 aacgattgat gcatcgtata cgaaagagaa ttttgaaaat gaagtgccat ttgcgggatt 721 tgattcagaa ttagctttat cttcaattaa acgtttaaaa gaagtggtga tgaaagagaa 781 gccgattgtt ttctttggac atgatataga gcaggaaagg ggatgtaaag tgttccctga 841 atatatagct gcaaacgacg aaaactacgc tttagtagct taataacgct gatagtgcta 901 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 1021 ccttcgggtg ggcctttctg cgtttatata ctagagttta tggctagctc agtcctaggt 1081 acaatgctag ctactagaga aagaggagaa atactagatg agcacaaaaa agaaaccatt 1141 aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa 1201 tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt 1261 tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa 1321 aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat 1381 gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc 1441 tcatgttcag gcagggatgt tctcacctga gcttagaacc tttaccaaag gtgatgcgga 1501 gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg 1561 taattccatg accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct 1621 cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga 1681 tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa 1741 cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc 1801 tagtcagtgg cctgaagaga cgtttggctg atactagagc caggcatcaa ataaaacgaa 1861 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1921 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaac 1981 accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgc //