LOCUS       BBa_K2173003            2025 bp    DNA     linear       23-May-2024
DEFINITION  aiiA_lambda-CI
ACCESSION   BBa_K2173003
VERSION     BBa_K2173003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=LuxR-AHL Binding Box
     misc_feature    42..47
                     /label=Pseudo-Pribnow Box
     misc_feature    52..68
                     /label=Lambda Repressor OR1
     misc_feature    51..51
                     /label=Transcription Start Site
     promoter        1..68
                     /label=BBa_K415032
     RBS             77..91
                     /label=RBS-1\Strong
     RBS             84..88
                     /label=RBS
     misc_feature    77..91
                     /label=BBa_B0030
     RBS             77..91
                     /label=BBa_B0030
     misc_feature    98..100
                     /label=start
     misc_feature    881..886
                     /label=2
     misc_feature    848..880
                     /label=LVA
     CDS             98..847
                     /label=aiiA
     misc_feature    98..886
                     /label=BBa_C0060
     misc_feature    887..911
                     /label=Help:Barcodes
     CDS             98..886
                     /label=BBa_C0060
     stem_loop       931..974
                     /label=stem_loop
     misc_feature    920..999
                     /label=BBa_B0010
     terminator      920..999
                     /label=BBa_B0010
     stem_loop       1015..1034
                     /label=T7 TE
     misc_feature    1041..1041
                     /label=stop
     polyA_site      1035..1048
                     /label=polya
     misc_feature    1008..1048
                     /label=BBa_B0012
     terminator      1008..1048
                     /label=BBa_B0012
     terminator      920..1048
                     /label=BBa_B0015
     promoter        1057..1091
                     /label=BBa_J23114
     misc_feature    1104..1107
                     /label=conserved
     RBS             1100..1111
                     /label=BBa_B0034
     CDS             1118..1831
                     /label=cIts
     CDS             1118..1831
                     /label=BBa_K098997
     stem_loop       1851..1894
                     /label=stem_loop
     misc_feature    1840..1919
                     /label=BBa_B0010
     terminator      1840..1919
                     /label=BBa_B0010
     stem_loop       1935..1954
                     /label=T7 TE
     misc_feature    1961..1961
                     /label=stop
     polyA_site      1955..1968
                     /label=polya
     misc_feature    1928..1968
                     /label=BBa_B0012
     terminator      1928..1968
                     /label=BBa_B0012
     terminator      1840..1968
                     /label=BBa_B0015
     promoter        2014..2019
                     /label=-10
     promoter        1991..1996
                     /label=-35
     misc_feature    2001..2017
                     /label=OR1
     misc_feature    1977..1993
                     /label=OR2
     misc_feature    1977..2025
                     /label=BBa_R0051
     promoter        1977..2025
                     /label=BBa_R0051
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atacctctgg
       61 cggtgatata ctagagatta aagaggagaa atactagatg acagtaaaga agctttattt
      121 cgtcccagca ggtcgttgta tgttggatca ttcgtctgtt aatagtacat taacaccagg
      181 agaattatta gacttaccgg tttggtgtta tcttttggag actgaagaag gacctatttt
      241 agtagataca ggtatgccag aaagtgcagt taataatgaa ggtcttttta acggtacatt
      301 tgtcgaaggg caggttttac cgaaaatgac tgaagaagat agaatcgtga atattttaaa
      361 acgggttggt tatgagccgg aagaccttct ttatattatt agttctcact tgcattttga
      421 tcatgcagga ggaaatggcg cttttataaa tacaccaatc attgtacagc gtgctgaata
      481 tgaggcggcg cagcatagcg aagaatattt gaaagaatgt atattgccga atttaaacta
      541 caaaatcatt gaaggtgatt atgaagtcgt accaggagtt caattattgc atacaccagg
      601 ccatactcca gggcatcaat cgctattaat tgagacagaa aaatccggtc ctgtattatt
      661 aacgattgat gcatcgtata cgaaagagaa ttttgaaaat gaagtgccat ttgcgggatt
      721 tgattcagaa ttagctttat cttcaattaa acgtttaaaa gaagtggtga tgaaagagaa
      781 gccgattgtt ttctttggac atgatataga gcaggaaagg ggatgtaaag tgttccctga
      841 atatatagct gcaaacgacg aaaactacgc tttagtagct taataacgct gatagtgcta
      901 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1021 ccttcgggtg ggcctttctg cgtttatata ctagagttta tggctagctc agtcctaggt
     1081 acaatgctag ctactagaga aagaggagaa atactagatg agcacaaaaa agaaaccatt
     1141 aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa
     1201 tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt
     1261 tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa
     1321 aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat
     1381 gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc
     1441 tcatgttcag gcagggatgt tctcacctga gcttagaacc tttaccaaag gtgatgcgga
     1501 gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg
     1561 taattccatg accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct
     1621 cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga
     1681 tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa
     1741 cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc
     1801 tagtcagtgg cctgaagaga cgtttggctg atactagagc caggcatcaa ataaaacgaa
     1861 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1921 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaac
     1981 accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgc
//