LOCUS       BBa_K2173008            1996 bp    DNA     linear       23-Dec-2024
DEFINITION  aiiA_mRFP1
ACCESSION   BBa_K2173008
VERSION     BBa_K2173008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=LuxR-AHL Binding Box
     misc_feature    42..47
                     /label=Pseudo-Pribnow Box
     misc_feature    52..68
                     /label=Lambda Repressor OR1
     misc_feature    51..51
                     /label=Transcription Start Site
     promoter        1..68
                     /label=BBa_K415032
     RBS             77..91
                     /label=RBS-1\Strong
     RBS             84..88
                     /label=RBS
     misc_feature    77..91
                     /label=BBa_B0030
     RBS             77..91
                     /label=BBa_B0030
     misc_feature    98..100
                     /label=start
     misc_feature    881..886
                     /label=2
     misc_feature    848..880
                     /label=LVA
     CDS             98..847
                     /label=aiiA
     misc_feature    98..886
                     /label=BBa_C0060
     misc_feature    887..911
                     /label=Help:Barcodes
     CDS             98..886
                     /label=BBa_C0060
     stem_loop       931..974
                     /label=stem_loop
     misc_feature    920..999
                     /label=BBa_B0010
     terminator      920..999
                     /label=BBa_B0010
     stem_loop       1015..1034
                     /label=T7 TE
     misc_feature    1041..1041
                     /label=stop
     polyA_site      1035..1048
                     /label=polya
     misc_feature    1008..1048
                     /label=BBa_B0012
     terminator      1008..1048
                     /label=BBa_B0012
     terminator      920..1048
                     /label=BBa_B0015
     misc_feature    1057..1077
                     /label=LuxR-AHL Binding Box
     misc_feature    1098..1103
                     /label=Pseudo-Pribnow Box
     misc_feature    1108..1124
                     /label=Lambda Repressor OR1
     misc_feature    1107..1107
                     /label=Transcription Start Site
     promoter        1057..1124
                     /label=BBa_K415032
     RBS             1133..1147
                     /label=RBS-1\Strong
     RBS             1140..1144
                     /label=RBS
     misc_feature    1133..1147
                     /label=BBa_B0030
     RBS             1133..1147
                     /label=BBa_B0030
     CDS             1154..1828
                     /label=mrfp1
     misc_feature    1835..1859
                     /label=Help:Barcodes
     CDS             1154..1859
                     /label=BBa_E1010
     stem_loop       1879..1922
                     /label=stem_loop
     misc_feature    1868..1947
                     /label=BBa_B0010
     terminator      1868..1947
                     /label=BBa_B0010
     stem_loop       1963..1982
                     /label=T7 TE
     misc_feature    1989..1989
                     /label=stop
     polyA_site      1983..1996
                     /label=polya
     misc_feature    1956..1996
                     /label=BBa_B0012
     terminator      1956..1996
                     /label=BBa_B0012
     terminator      1868..1996
                     /label=BBa_B0015
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atacctctgg
       61 cggtgatata ctagagatta aagaggagaa atactagatg acagtaaaga agctttattt
      121 cgtcccagca ggtcgttgta tgttggatca ttcgtctgtt aatagtacat taacaccagg
      181 agaattatta gacttaccgg tttggtgtta tcttttggag actgaagaag gacctatttt
      241 agtagataca ggtatgccag aaagtgcagt taataatgaa ggtcttttta acggtacatt
      301 tgtcgaaggg caggttttac cgaaaatgac tgaagaagat agaatcgtga atattttaaa
      361 acgggttggt tatgagccgg aagaccttct ttatattatt agttctcact tgcattttga
      421 tcatgcagga ggaaatggcg cttttataaa tacaccaatc attgtacagc gtgctgaata
      481 tgaggcggcg cagcatagcg aagaatattt gaaagaatgt atattgccga atttaaacta
      541 caaaatcatt gaaggtgatt atgaagtcgt accaggagtt caattattgc atacaccagg
      601 ccatactcca gggcatcaat cgctattaat tgagacagaa aaatccggtc ctgtattatt
      661 aacgattgat gcatcgtata cgaaagagaa ttttgaaaat gaagtgccat ttgcgggatt
      721 tgattcagaa ttagctttat cttcaattaa acgtttaaaa gaagtggtga tgaaagagaa
      781 gccgattgtt ttctttggac atgatataga gcaggaaagg ggatgtaaag tgttccctga
      841 atatatagct gcaaacgacg aaaactacgc tttagtagct taataacgct gatagtgcta
      901 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1021 ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt acaggtttac
     1081 gcaagaaaat ggtttgttat agtcgaatac ctctggcggt gatatactag agattaaaga
     1141 ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt
     1201 tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg
     1261 tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt
     1321 cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc
     1381 ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt
     1441 tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg
     1501 tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat
     1561 gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc
     1621 tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga
     1681 agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga
     1741 catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg
     1801 tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct
     1861 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1921 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1981 cctttctgcg tttata
//