LOCUS BBa_K2176001 4491 bp DNA linear 10-May-2024 DEFINITION Cassette for the constitutive production of GAL4-KaiCp and LexA-SasA, linked by a self-cleaving P2A ACCESSION BBa_K2176001 VERSION BBa_K2176001.1 FEATURES Location/Qualifiers misc_feature 6..27 /label=BioBrick Prefix misc_feature 4465..4485 /label=BioBrick Suffix promoter 40..438 /label=TEF1 Promoter misc_feature 4278..4464 /label=ADH1 Terminator misc_feature 458..478 /label=SV40 Nuclear Localization Signal misc_feature 479..817 /label=Gal4 Activator Domain misc_feature 818..826 /label=Linker Domain CDS 827..2383 /label=Phosphorylated KaiC Protein misc_feature 2384..2449 /label=P2A Self-Cleaving Linker misc_feature 2450..2470 /label=SV40 Nuclear Localization Signal CDS 2471..3076 /label=LexA Protein misc_feature 3077..3106 /label=Linker Domain CDS 3107..4270 /label=SasA Protein misc_feature 2471..2722 /label=DNA Binding Domain of LexA misc_feature 2042..2047 /label=Phosphomimetic Variable ORIGIN 1 aaaaagaatt cgcggccgct tctagaggct gctgctgcta tagcttcaaa atgtttctac 61 tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca 121 cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt taattacccg 181 tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc gtcgaaaaag 241 gcaataaaaa tttttatcac gtttcttttt cttgaaaatt tttttttttg atttttttct 301 ctttcgatga cctcccattg atatttaagt taataaacgg tcttcaattt ctcaagtttc 361 agtttcattt ttcttgttct attacaactt tttttacttc ttgctcatta gaaagaaagc 421 atagcaatct aatctaagtt ttaattacaa aggatctcca aaaaagaaga gaaaggtaaa 481 cttcaaccaa tctggtaaca tcgctgactc ttctttgtct ttcactttca ctaactcttc 541 taacggtcca aacttgatca ctactcaaac taactctcaa gctttgtctc aaccaatcgc 601 ttcttctaac gttcacgaca acttcatgaa caacgaaatc actgcttcta agatcgacga 661 cggtaacaac tctaagccat tgtctccagg ttggactgac caaactgctt acaacgcttt 721 cggtatcact actggtatgt tcaacactac tactatggac gacgtttaca actacttgtt 781 cgacgacgaa gacactccac caaacccaaa gaaggaagcg gcggcaatga cttccgctga 841 gatgactagc cctaataata attctgagca ccaagccatc gctaagatgc gtacgatgat 901 tgaaggattt gatgatatta gtcatggcgg tcttccaatc ggtcgatcga ccctcgttag 961 tggtacttca ggaaccggca agaccctttt ttctattcaa tttctctata acggtattat 1021 cgagtttgat gagcctggag ttttcgttac tttcgaagaa accccacaag atatcattaa 1081 aaacgcccgt agttttggct gggatttagc caagctggtc gatgagggca aactatttat 1141 tcttgatgct tcacccgatc cagaaggtca agaggttgtt ggcggcttcg atctctctgc 1201 tctgattgag cggattaatt atgcaattca aaagtatcga gcgcgtaggg tttcaattga 1261 ctcggtcacg tccgttttcc agcaatatga tgcctcttct gtggttcgcc gcgaactctt 1321 taggttggta gctcgcctaa aacaaattgg tgcaactacg gtcatgacca ccgagcgtat 1381 cgaggaatat ggcccaatcg ctcgttacgg tgttgaggaa tttgtctccg ataacgtcgt 1441 gattctccgc aacgttttgg aaggagagcg ccgtcgccgc accctcgaaa tcctcaagct 1501 acgtggcacc agccacatga aaggtgaata tccattcacg attacggatc atggcatcaa 1561 tatcttcccc ctcggagcaa tgcgccttac gcagcgatcg tcgaacgtgc gtgtttcatc 1621 tggtgtcgtc cgactcgatg aaatgtgtgg tggtggcttc tttaaggact caatcattct 1681 ggcaactggc gctacaggca ctggtaaaac tctgttagtt agccgtttcg ttgagaatgc 1741 ttgtgctaac aaagagagag cgattctgtt cgcttatgaa gagtcacgag ctcagctgct 1801 ccgcaatgcc tattcatggg gaatggactt tgaggagatg gagcgccaaa acctcctcaa 1861 aattgtttgc gcctatcctg aatccgcagg ccttgaagac catttgcaga ttattaagtc 1921 ggagatcaat gactttaagc cagctcgtat tgcaatcgac tccctctctg ctttggcgag 1981 gggcgttagc aacaatgcct tccgccaatt tgtaattggt gtcactggct acgcgaaaca 2041 agaagaaatc acgggactat tcacaaatac cagtgatcaa tttatgggag cgcattcgat 2101 tactgactcc catatcgaag aaattacgga tacgattatc ttgctccaat acgtcgagat 2161 tcgtggcgaa atgtcccgcg ccattaacgt cttcaagatg cgcggatctt ggcatgacaa 2221 agcaatccgc gaatttatga tcagcgacaa aggtccagac atcaaggatt ctttcaggaa 2281 ctttgagaga attatttcag gttcgccaac acgtattacc gtcgatgaga aaagcgaact 2341 ctcgcgaatt gtgcgcggcg ttcaagaaaa agggcccgag agcggttctg gtgctactaa 2401 cttctctttg ttgaagcaag ctggtgacgt tgaagaaaac ccaggtccac caaaaaagaa 2461 gagaaaggta atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga 2521 tcacatcagc cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt 2581 ccgttcccca aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga 2641 aattgtttcc ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc 2701 gctggtaggt cgtgtggctg ccggtgaacc acttctggcg caacagcata ttgaaggtca 2761 ttatcaggtc gatccttcct tattcaagcc gaatgctgat ttcctgctgc gcgtcagcgg 2821 gatgtcgatg aaagatatcg gcattatgga tggtgacttg ctggcagtgc ataaaactca 2881 ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg 2941 cctgaaaaaa cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat 3001 tgtcgttgac cttcgtcagc agagcttcac cattgaaggg ctggcggttg gggttattcg 3061 caacggcgac tggctgggct ccggctccgg cggctccggc tccggcatgg gagagtctct 3121 gtcaccacaa gcactggctc aaccgttgct actgcaactg ttcgtcgata cccggcccct 3181 gtcacagcac attgtgcagc gggttaaaaa tattttggca gcagtagagg caaccgtccc 3241 catcagcttg caggtgatca atgtggcgga ccagccacaa ctggtggagt actaccgcct 3301 agtcgtcacg cctgccctgg ttaaaattgg tccaggctct cgccaagttc tgagtggcat 3361 cgacctcacc gatcaattag ccaaccagtt gccccagtgg ctggttcagc aagaggcctt 3421 ttttgccgat cgagagccac ctgaagtcaa cattccgttc acggagctag gccaacccga 3481 gacccccgcg ttgcagcagg ctgatgcctt ttttcagctt cagcaacaat acgctgatct 3541 ctcggagcgg acaaaattcc ttgagcaggt cattgctctc gtcgcccacg acctccgcaa 3601 tccgctaacc gctgctcttt tggcggtcga caccattcaa atccgcagtc aatccttttc 3661 tgtggcgaca gccaaggaaa tgcagggact gtgcagtctg ttcgatcagg cacgatcgca 3721 attgcgtgaa atcgagcgca tgattgccga gattttggag gcaactcgcc actctggcga 3781 aagcttgcgg atcaatcccc gcgaagtcgt ctttgagccg ctcctacaac aggttctgga 3841 acagttgcat gaacgctggc ggagtaagca gcagcagttg atcacggatg ttccaggcga 3901 cctgcccacg ctctacgccg accctgaccg tctgcggcag gttctggtca atctgctaga 3961 caacgccatc aaatacactc cgcccggggg cacaatcacg atcgcagcgc tccatcgcac 4021 gagccagaaa gtgcagatca gcatcagcga cacgggctct ggcatccctc gcgaccagct 4081 cagcgtcatt ttcaagaact tggtgcggct ttcccgcgat agcagccaag agggctacgg 4141 cattggacta tcggtttgcc agcgaattgt tcaggcccac tttggccgca tctgggttgc 4201 ttcggaactg ggccaaggca gcaccttcca cttcacgatg ccggtttatc gctacaccat 4261 gccctgctaa ggatctgcga atttcttatg atttatgatt tttattatta aataagttat 4321 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt 4381 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt atgaggtcgc 4441 tcttattgac cacacctcta ccggctgcag cggccgctac tagtattttt t //