BBa_K2179003
1
Cld(-SP)
Chlorite Dismutase CDS+RBS from I. dechloratans with His-tag
2016-10-20T11:00:00Z
2016-10-29T02:22:04Z
Derived from the soil bacterium Ideonella deschloratans
This an engineer derivative of the Chlorite dismutase coding sequence from the soil bacterium Ideonella Deschloratans (Thorell et al. 2003). This enzyme coverts chlorite (ClO2) to oxygen and chloride. The 5' sequence that encodes the periplasmic localization signal has been removed. In addition, a His10x tag has been appended to the c-terminus. An internal BsaI site was eliminated by a a silent C-to-G substution at the third codon position at position 198 of the protein coding sequence. The CDS ends with the. consequetiv codons two stop codons TGA. The part is also flanked at either end by two BsaI sites at either end that are internal to the prefix and suffix of pSB1C3. These sites produce non-palindromic sticky end that are differ from one in sequence. They can be useful for the precise unidirectional ligation of a single gene copy to an an appropriately designed part or plasmid backbone. The CDS is preceded with a strong RBS derived form the the expression plasmid PCB-38-441 which is commercially available from DNA 2.0.
Thorell, H.D, Karlsson, J., Portelius, E.and Nilsson, T. Biochimica et Biophysica Acta 1577 (2002) 445???451
false
false
_2650_
31733
31958
9
true
see above
false
michael ellison
annotation2527368
1
chlorite dismutase
range2527368
1
27
800
annotation2527367
1
rbs
range2527367
1
11
22
annotation2527370
1
BsaI
range2527370
1
6
11
annotation2527418
1
His Tag GGHHHHHHHHHH
range2527418
1
765
800
annotation2527377
1
BsaI
range2527377
1
807
813
BBa_K2179003_sequence
1
ttttcgagaccttaggaggtaaacatatgaataccccagttgatcgggcgaagatactcagcgcgccaggcgtgtttgtggcgttctcaacttacaaaattcgtcccgactacttcaaagttgcattggctgaacgcaaaggtgcagcagatgaagtgatggcggtcttggaaaagcacaaagaaaaagtgattgtcgacgcctacctgacgcgcggctatgaagccaagagcgactacttcctgcgcgttcatgcctacgatgccgtagcggcgcaggcctttctggtcgatttccgcgccacccgctttggcatgtactcggatgtcacggagagcctggtgggtatcaccaaggcgctaaactatatctccaaggacaagtcgcccgacctcaacaaggggctttccggtgctacctacgccggtgatgcaccgcgctttgccttcatgattccggtcaagaaaaacgctgattggtggaacctgacggacgagcagcgcctcaaggagatggagactcatacgcttccgacgctgccctttctggtcaacgtcaagcgcaagctctaccactcgacggggctcgacgataccgattttattacctacttcgagacgaacgacctcggagccttcaacaacctgatgctgtcgctggccaaggtgccggagaacaagtatcacgtgcgctggggcaatccgaccgtgctgggtaccatccagcccatcgagaacctcgtcaagacgctgtcgatgggcaatggcggtcatcaccaccatcatcaccatcaccaccattgatgaggtctcacccc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z