LOCUS BBa_K218000 3825 bp DNA linear 23-Dec-2024 DEFINITION LuxPQ from Vibrio harveyi ACCESSION BBa_K218000 VERSION BBa_K218000.1 FEATURES Location/Qualifiers misc_feature 30..32 /label=start of LuxP misc_feature 1127..1129 /label=start of LuxQ misc_feature 1125..1127 /label=stop for LuxP CDS 30..1127 /label=LuxP misc_feature 3704..3706 /label=stop for LuxQ CDS 1127..3706 /label=LuxQ misc_feature 599..599 /label=Silent mutation to remove EcoRI site misc_feature 1465..1465 /label=Silent mutation to remove EcoRI site misc_feature 1618..1618 /label=Silent mutation to remove XbaI site ORIGIN 1 atgctcgata aaaactaaaa gagcaataga tgaagaaagc gttactattt tccctgattt 61 ctatggtcgg tttttctcca gcgtctcaag caacacaagt tttgaatggg tactggggtt 121 atcaagagtt tttggacgag tttcccgagc aacgaaatct gaccaatgct ttatcagaag 181 cagtacgagc acagccggtc ccactgtcta aaccgacaca acgcccgatt aaaatatcag 241 tggtttaccc aggacagcaa gtttcagatt actgggtaag aaatattgca tcattcgaaa 301 aacgtttgta taagttgaat attaactacc aactgaacca agtgtttact cgtccaaatg 361 ctgatatcaa gcaacaaagc ttgtcattaa tggaagcgct caagagcaaa tcggattact 421 tgattttcac gcttgatacg acaagacacc gtaaatttgt tgagcacgtt ttggactcaa 481 cgaacaccaa attgatcttg caaaatatca ctacaccagt ccgtgagtgg gacaaacatc 541 aaccgttttt atatgtcgga tttgaccacg cagaaggcag tcgtgaatta gcaacagagt 601 tcggaaagtt cttcccaaaa cacacatatt acagtgtgct ctacttttct gaaggttata 661 ttagcgatgt gagaggtgat acttttattc accaagtaaa ccgtgataat aactttgagc 721 tacaatcagc gtattacacg aaggcaacca agcaatccgg ctatgatgct gcgaaagcga 781 gtttagcaaa acatccagat gttgatttta tctatgcatg ttcgaccgac gtagcattag 841 gtgcagtaga cgcactggct gagttgggac gtgaagatat tatgatcaat ggctggggtg 901 gaggctctgc tgagttagac gctatccaga agggtgattt agacatcacc gtcatgcgta 961 tgaatgatga cactggcata gccatggcag aagcgattaa gtgggacttg gaagataaac 1021 cagttccgac cgtatactca ggtgactttg aaatcgtaac aaaggcagat tcaccggaga 1081 gaatcgaagc gctgaaaaag cgcgcattta gatattcaga taattgatga caacaacgcg 1141 atcaaacatt aaaaagcgtc gctcgctggc gacgctcata acaaagatca tcattttagt 1201 tcttgcccca attattctgg ggattttcat tcagagctat tacttctcca agcaaatcat 1261 ttggcaagaa gtagaccgaa ccaaacagca aacctctgca ctgatccaca atatatttga 1321 tagccacttt gcggcgatcc agatacatca tgacagtaat tccaagagcg aagtcattcg 1381 tgatttctac actgatcgcg acacggatgt gctcaacttt ttcttcctca gtatcgacca 1441 aagcgatccg tcgcacacac cagagttccg ttttctaacg gaccacaaag gcatcatttg 1501 ggacgatgga aatgcgcatt tctatggtgt gaacgacctt atccttgata gccttgccaa 1561 tcgggtcagt ttcagtaaca actggtatta cattaatgtc atgacctcca ttggttcgag 1621 acacatgctc gtgcgccgtg tgccgatcct agacccttca acaggagagg tgcttggttt 1681 ctcatttaat gccgtcgtct tagacaacaa cttcgctttg atggaaaagc tcaagagtga 1741 aagtaacgtc gacaatgtgg tgctggttgc taatagcgtt cctttagcaa actctttgat 1801 tggtgatgag ccatataacg ttgctgatgt attgcagcgt aaaagttcag acaaaagact 1861 cgataagctg ttggtaatag aaacgccaat cgtcgtaaat gcagtgacta ccgagctttg 1921 cttgttgacg gtacaagaca atcagagtgt ggtgacatta caaatccaac atattctagc 1981 catgcttgca tcgatcatcg gtatgatcat gattgcctta atgagtaggg aatggattga 2041 gagtaaagtt tcggcgcagt tagaatcttt gatgtcttac acccgctctg ctcgtgagga 2101 aaaagggttt gaacgatttg gcggttcgga tattgaagag tttgatcaca tcggttcaac 2161 ccttgaaagt acattcgaag agcttgaagc gcagaagaag tcgttccgag atctgtttaa 2221 ttttgcctta tcacccatca tggtttggtc tgaagagagt gtcctgattc agatgaaccc 2281 tgccgcgcgc aaagaattag tgatcgaaga cgatcatgaa atcatgcatc cggtcttcca 2341 aggctttaaa gagaaattga ccccacacct caaaatggcg gctcaaggtg cgacgttgac 2401 tggggtgaac gtgcctattg gtaataagat ctaccgatgg aacttgtcgc caattcgtgt 2461 tgatggcgat atcagtggca ttattgtgca aggccaagac attacaacac ttatcgaagc 2521 cgagaagcag agtaacattg cgcgtagaga agcagaaaaa tcggcgcaag cacgtgcaga 2581 cttccttgct aaaatgagcc atgaaattcg tacgccaatc aacggcattt taggtgtcgc 2641 ccaattattg aaagattctg tcgatacaca agagcagaag aatcaaatcg acgtcctgtg 2701 ccacagtggc gagcacttgc ttgcagtact gaacgatatt ctcgatttct caaagataga 2761 gcagggcaag ttcaatattc agaaacaccc gttctccttc accgatacca tgcgtacatt 2821 ggaaaatatt tatcgtccga tttgcacaaa taagggggtg gagttggtca tcgagaatga 2881 gcttgacccg aatgttgaaa tcttcaccga tcaagtccgc ttgaatcaga ttctatttaa 2941 cttagtgagt aatgccgtta agttcacgcc gattggctcg attcgactgc acgcagaact 3001 tgaacaattc tatggtgcgg agaacagcgt gttagttgtg gaactgactg atactggcat 3061 cggcattgaa agcgataagc tcgaccaaat gttcgaacct tttgtgcaag aagagtcgac 3121 aaccacacgc gaatatggcg gtagcggcct aggtttgacc atcgttaaga acctagtcga 3181 tatgttagaa ggtgatgttc aggtccgcag tagcaagggg ggggggacaa catttgttat 3241 aacacttcca gtaaaagatc gtgagcgtgt cttaaggcct ctggaggtca gtcaacgtat 3301 caagccggaa gccttgtttg atgaaagttt aaaagtgcta ctggtggaag ataaccatac 3361 caatgcgttt atccttcagg ctttctgtaa gaagtataaa atgcaggtgg attgggcgaa 3421 agatgggctg gacgcgatgg agctcctttc tgataccacc tacgatctga tcctcatgga 3481 taaccaatta ccccaccttg gtggtattga gaccacgcac gagattcgcc agaacttgag 3541 gcttggaacg ccaatttacg cgtgtacagc agacaccgcg aaagaaacca gtgatgcgtt 3601 tatggcggca ggtgcaaact atgtcatgct gaagccaatt aaagagaatg cgttacatga 3661 ggcgtttgtc gatttcaaac aacgtttctt ggtagaaaga acctaacggt ttaatggcag 3721 tgataagtta ggggctgaag ttaaataata aaagtaaaga agggagcgta atgcactaat 3781 gccgttcact taaggtgatc ggcattgttt tttctagggt atcgg //