LOCUS       BBa_K2180004            1466 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter +FUR regulatory sequence + RBS + LacI-LVA + Terminator
ACCESSION   BBa_K2180004
VERSION     BBa_K2180004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=BamHI
     promoter        16..136
                     /label=PliaG
     misc_feature    137..142
                     /label=ClaI
     misc_feature    143..163
                     /label=Fur Box
     misc_feature    164..169
                     /label=PscI
     RBS             170..180
                     /label=RBS
     misc_feature    188..193
                     /label=NcoI
     promoter        1..193
                     /label=BBa_K2180007
     CDS             200..1351
                     /label=LacI + LVA
     CDS             200..1351
                     /label=BBa_K2180009
     misc_feature    1360..1365
                     /label=BmtI
     misc_feature    1366..1406
                     /label=Transcription terminator
     misc_feature    1415..1460
                     /label=Transcription terminator'
     misc_feature    1461..1466
                     /label=AvrII
     terminator      1360..1466
                     /label=BBa_K2180008
ORIGIN
        1 ggatcccaag cttggcaaaa atcagaccag acaaaagcgg caaatgaata agcggaacgg
       61 ggaaggattt gcggtcaagt ccttcccttc cgcacgtatc aattcgcaag cttttccttt
      121 ataatagaat gaatgaatcg attgataatg ataatcatta tcaacatgta aaggaggtgt
      181 ctttatccca tggtactaga tggtaaacgt gaaaccagtg acattgtacg atgttgctga
      241 gtatgcaggc gtcagctatc aaactgtaag tcgagtagtt aaccaagcca gccatgtgag
      301 cgcaaaaacg cgcgagaagg tggaagcggc gatggctgag ttaaactata taccaaatag
      361 agtcgctcag caattagccg gcaaacagtc cctgttgatc ggtgtagcga cctcctctct
      421 ggcattgcat gctccgtctc agatcgtggc agcaattaag tcacgtgctg atcaattagg
      481 agcttctgtt gttgtcagca tggtggaaag gagcggagtc gaggcgtgca aagcggcggt
      541 tcataaccta ctagcccagc gcgtgtctgg gcttattata aattaccccc tggatgatca
      601 agatgcaatc gcggttgaag cagcttgtac gaacgtccca gccttgttcc tcgacgtcag
      661 tgaccagacg ccgattaatt ccatcatctt tagccacgag gacggaactc gcctcggcgt
      721 ggagcatctt gtcgcgttgg gacaccagca gattgctcta ctcgcggggc cgttaagttc
      781 tgtatcagca cgtttaagac tcgcaggctg gcataaatat ttgaccagaa atcaaattca
      841 accaattgct gaacgcgaag gagattggtc ggcaatgagc ggcttccagc agacgatgca
      901 aatgctgaat gaaggtattg ttcctaccgc aatgttagtg gccaacgacc aaatggcgct
      961 cggtgcgatg cgtgcaatca ccgaatcagg cttgcgtgtg ggggctgata tctccgtggt
     1021 cgggtatgac gatactgaag attcaagctg ttatattccg cctttgacaa ctataaaaca
     1081 agattttaga ctgctaggcc agacatcagt agatcgactg ctgcaacttt cccaggggca
     1141 agcggttaaa ggaaaccaac tgttaccggt atctctcgtc aaacgcaaga caacattagc
     1201 cccgaatacc caaacagcat ccccgcgcgc tctcgctgac tcacttatgc agcttgcccg
     1261 gcaggtcagt agactggaat cagggcaggc agctaatgac gaaaattatg cccttgtagc
     1321 ctaatgactt taataatgct gatgcaggtc atactagagg ctagctcaca ctggctcacc
     1381 ttcgggtggg cctttctgcg tttatatact agagagagaa tataaaaagc cagattatta
     1441 atccggcttt tttattattt cctagg
//