BBa_K2180007 1 BBa_K2180007 PFur - Promoter Fur for B. subtilis 2016-08-04T11:00:00Z 2016-10-13T10:58:33Z This part is based on a previous IGEM part (add part). We add the Fur Box site we found (add site) This part is made of PleiaG promoter, a fur (Ferric Uptake Regulator) binding sites and a RBS. There is a ClaI restriction site before the Fur Box (Fur Binding Site) and a PscI restriction site after. They allows us to remplace Fur Box by another operating sequence. A BamHI restriction sites is present before the PliaG sequence that may be used to insert the whole sequence. We use this part in our project to promote the LacI + LVA expression under the control of Fur. false false _2651_ 25719 25719 9 false We had to check the compatibility of the promoter with the Fur Box. false Sofiane Safi-Stibler annotation2481412 1 RBS range2481412 1 170 180 annotation2481417 1 NcoI range2481417 1 188 193 annotation2481408 1 PliaG range2481408 1 16 136 annotation2481410 1 Fur Box range2481410 1 143 163 annotation2481407 1 BamHI range2481407 1 1 6 annotation2481409 1 ClaI range2481409 1 137 142 annotation2481411 1 PscI range2481411 1 164 169 BBa_K2180004 1 BBa_K2180004 Promoter +FUR regulatory sequence + RBS + LacI-LVA + Terminator 2016-08-04T11:00:00Z 2016-10-29T05:30:41Z The sequence is based on a previous IGEM part (add part) coding for LacI + LVA for E. coli Coding sequence of LacI protein + LVA (rapid degradation tail) wich improve the switch time and make it more sensitive. false false _2651_ 32196 25719 9 false We had to improve the codon usage for B. subtilis false Sofiane Safi-Stibler component2521324 1 BBa_K2180008 component2521319 1 BBa_K2180009 component2521317 1 BBa_K2180007 annotation2521319 1 BBa_K2180009 range2521319 1 200 1351 annotation2521317 1 BBa_K2180007 range2521317 1 1 193 annotation2521324 1 BBa_K2180008 range2521324 1 1360 1466 BBa_K2180008 1 BBa_K2180008 Terminator 2016-08-04T11:00:00Z 2016-10-13T10:53:37Z This is a double terminator we use in our project for the pigment production. He is made of a transcriptional terminator in forward and reverse direction. There is a BmtI restriction site before the first terminator (forward) and an AvrII restriction site after the second terminator (reverse). false false _2651_ 25719 25719 9 false false Sofiane Safi-Stibler annotation2481416 1 AvrII range2481416 1 102 107 annotation2481414 1 Transcription terminator range2481414 1 7 47 annotation2481413 1 BmtI range2481413 1 1 6 annotation2481415 1 Transcription terminator' range2481415 1 56 101 BBa_K2180009 1 BBa_K2180009 LacI-LVA for B. subtilis 2016-08-04T11:00:00Z 2016-10-19T08:26:00Z This is the assembly of our basic parts BBa_K2180004 , BBa_K2180007 without the Fur Box and BBa_K2180008 We delete the Fur Box in order to use it like a normal promoter. The NcoI restriction site is overlapping parts BBa_K2180004 and BBa_K2180007 at the Start codon of LacI + LVA. false false _2651_ 25719 25719 9 false false Sofiane Safi-Stibler annotation2521118 1 LacI + LVA range2521118 1 1 1152 BBa_K2180004_sequence 1 ggatcccaagcttggcaaaaatcagaccagacaaaagcggcaaatgaataagcggaacggggaaggatttgcggtcaagtccttcccttccgcacgtatcaattcgcaagcttttcctttataatagaatgaatgaatcgattgataatgataatcattatcaacatgtaaaggaggtgtctttatcccatggtactagatggtaaacgtgaaaccagtgacattgtacgatgttgctgagtatgcaggcgtcagctatcaaactgtaagtcgagtagttaaccaagccagccatgtgagcgcaaaaacgcgcgagaaggtggaagcggcgatggctgagttaaactatataccaaatagagtcgctcagcaattagccggcaaacagtccctgttgatcggtgtagcgacctcctctctggcattgcatgctccgtctcagatcgtggcagcaattaagtcacgtgctgatcaattaggagcttctgttgttgtcagcatggtggaaaggagcggagtcgaggcgtgcaaagcggcggttcataacctactagcccagcgcgtgtctgggcttattataaattaccccctggatgatcaagatgcaatcgcggttgaagcagcttgtacgaacgtcccagccttgttcctcgacgtcagtgaccagacgccgattaattccatcatctttagccacgaggacggaactcgcctcggcgtggagcatcttgtcgcgttgggacaccagcagattgctctactcgcggggccgttaagttctgtatcagcacgtttaagactcgcaggctggcataaatatttgaccagaaatcaaattcaaccaattgctgaacgcgaaggagattggtcggcaatgagcggcttccagcagacgatgcaaatgctgaatgaaggtattgttcctaccgcaatgttagtggccaacgaccaaatggcgctcggtgcgatgcgtgcaatcaccgaatcaggcttgcgtgtgggggctgatatctccgtggtcgggtatgacgatactgaagattcaagctgttatattccgcctttgacaactataaaacaagattttagactgctaggccagacatcagtagatcgactgctgcaactttcccaggggcaagcggttaaaggaaaccaactgttaccggtatctctcgtcaaacgcaagacaacattagccccgaatacccaaacagcatccccgcgcgctctcgctgactcacttatgcagcttgcccggcaggtcagtagactggaatcagggcaggcagctaatgacgaaaattatgcccttgtagcctaatgactttaataatgctgatgcaggtcatactagaggctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagg BBa_K2180009_sequence 1 atggtaaacgtgaaaccagtgacattgtacgatgttgctgagtatgcaggcgtcagctatcaaactgtaagtcgagtagttaaccaagccagccatgtgagcgcaaaaacgcgcgagaaggtggaagcggcgatggctgagttaaactatataccaaatagagtcgctcagcaattagccggcaaacagtccctgttgatcggtgtagcgacctcctctctggcattgcatgctccgtctcagatcgtggcagcaattaagtcacgtgctgatcaattaggagcttctgttgttgtcagcatggtggaaaggagcggagtcgaggcgtgcaaagcggcggttcataacctactagcccagcgcgtgtctgggcttattataaattaccccctggatgatcaagatgcaatcgcggttgaagcagcttgtacgaacgtcccagccttgttcctcgacgtcagtgaccagacgccgattaattccatcatctttagccacgaggacggaactcgcctcggcgtggagcatcttgtcgcgttgggacaccagcagattgctctactcgcggggccgttaagttctgtatcagcacgtttaagactcgcaggctggcataaatatttgaccagaaatcaaattcaaccaattgctgaacgcgaaggagattggtcggcaatgagcggcttccagcagacgatgcaaatgctgaatgaaggtattgttcctaccgcaatgttagtggccaacgaccaaatggcgctcggtgcgatgcgtgcaatcaccgaatcaggcttgcgtgtgggggctgatatctccgtggtcgggtatgacgatactgaagattcaagctgttatattccgcctttgacaactataaaacaagattttagactgctaggccagacatcagtagatcgactgctgcaactttcccaggggcaagcggttaaaggaaaccaactgttaccggtatctctcgtcaaacgcaagacaacattagccccgaatacccaaacagcatccccgcgcgctctcgctgactcacttatgcagcttgcccggcaggtcagtagactggaatcagggcaggcagctaatgacgaaaattatgcccttgtagcctaatgactttaataatgctgatgcaggtca BBa_K2180007_sequence 1 ggatcccaagcttggcaaaaatcagaccagacaaaagcggcaaatgaataagcggaacggggaaggatttgcggtcaagtccttcccttccgcacgtatcaattcgcaagcttttcctttataatagaatgaatgaatcgattgataatgataatcattatcaacatgtaaaggaggtgtctttatcccatgg BBa_K2180008_sequence 1 gctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z