BBa_K2180006
1
BBa_K2180006
PLacO - Promoter LacO for B. subtilis
2016-08-04T11:00:00Z
2016-10-13T10:58:17Z
The part is made of a previous IGEM part (add part)
This part is made of PlepA promoter, two LacI binding sites and a RBS. There is a PacI restriction site before LacI binding sites and a SacII restriction site after them. They allows us to remplace the LacI binding sites by another operating sequence. A SalI restriction sites is presents before the PlepA sequence that may be used to insert the whole sequence. We use this part in our project to promote the pigment expression under the control of LacI.
false
false
_2651_
25719
25719
9
false
false
Sofiane Safi-Stibler
annotation2481402
1
PacI restriction site
range2481402
1
180
187
annotation2481405
1
SacII restriction site
range2481405
1
230
235
annotation2481418
1
NdeI
range2481418
1
254
258
annotation2481406
1
RBS
range2481406
1
236
246
annotation2481400
1
SalI restriction site
range2481400
1
10
15
annotation2481404
1
LacI Operator O1
range2481404
1
209
229
annotation2481401
1
PlepA
range2481401
1
23
179
annotation2481403
1
LacI Operator O1
range2481403
1
188
208
BBa_K2180006_sequence
1
ccaagcttggtcgacgtcgaaaagtcaatgtatgaatggatacgggatatgaatcaataagtacgtgaaagagaaaagcaacccagatatgatagggaacttttctctttcttgttttacattgaatctttacaatcctattgatataatctaagctagtgtattttgcgtttaatagtttaattaaaattgtgagcggataacaattaattgtgagcggataacaattccgcggaaaggaggtgtctttatcatatg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z