BBa_K2180007
1
BBa_K2180007
PFur - Promoter Fur for B. subtilis
2016-08-04T11:00:00Z
2016-10-13T10:58:33Z
This part is based on a previous IGEM part (add part). We add the Fur Box site we found (add site)
This part is made of PleiaG promoter, a fur (Ferric Uptake Regulator) binding sites and a RBS. There is a ClaI restriction site before the Fur Box (Fur Binding Site) and a PscI restriction site after. They allows us to remplace Fur Box by another operating sequence. A BamHI restriction sites is present before the PliaG sequence that may be used to insert the whole sequence. We use this part in our project to promote the LacI + LVA expression under the control of Fur.
false
false
_2651_
25719
25719
9
false
We had to check the compatibility of the promoter with the Fur Box.
false
Sofiane Safi-Stibler
annotation2481417
1
NcoI
range2481417
1
188
193
annotation2481409
1
ClaI
range2481409
1
137
142
annotation2481412
1
RBS
range2481412
1
170
180
annotation2481408
1
PliaG
range2481408
1
16
136
annotation2481407
1
BamHI
range2481407
1
1
6
annotation2481410
1
Fur Box
range2481410
1
143
163
annotation2481411
1
PscI
range2481411
1
164
169
BBa_K2180007_sequence
1
ggatcccaagcttggcaaaaatcagaccagacaaaagcggcaaatgaataagcggaacggggaaggatttgcggtcaagtccttcccttccgcacgtatcaattcgcaagcttttcctttataatagaatgaatgaatcgattgataatgataatcattatcaacatgtaaaggaggtgtctttatcccatgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z