BBa_K2180011
1
BBa_K2180011
Arsenic Operator - ArsR Binding Site
2016-08-20T11:00:00Z
2016-10-13T11:22:04Z
Biobrick based on a previous biobrick made by the iGEM09_Newcastle Team (2009-10-14) :
http://parts.igem.org/Part:BBa_K174015
We add two restriction sites. SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allow ArsR to bind to DNA and repress transcription. In the presence of Heavy Metals such as Arsenic or Cadmium transcription is derepressed because of the fixation to ArsR.
Other metalic ions may bind to ArsR.
false
false
_2651_
25719
25719
9
false
false
Sofiane Safi-Stibler
annotation2481510
1
SacII
range2481510
1
1
6
annotation2481513
1
PacI
range2481513
1
59
66
annotation2481512
1
ArsR Binding Site
range2481512
1
33
58
annotation2481511
1
ArsR Binding Site
range2481511
1
7
32
BBa_K2180011_sequence
1
ccgcggagtaatcaaaataaattgatttattttttatttagttaaataaaactaatgattaattaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z