LOCUS       BBa_K2180012            2566 bp    DNA     linear       22-Nov-2024
DEFINITION  Promoter PlepA +FUR regulatory sequence + RBS + LacI-LVA + 
            Terminator + Pigment + RBS + Operateur La
ACCESSION   BBa_K2180012
VERSION     BBa_K2180012.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=BamHI
     promoter        16..136
                     /label=PliaG
     misc_feature    137..142
                     /label=ClaI
     misc_feature    143..163
                     /label=Fur Box
     misc_feature    164..169
                     /label=PscI
     RBS             170..180
                     /label=RBS
     misc_feature    188..193
                     /label=NcoI
     promoter        1..193
                     /label=BBa_K2180007
     CDS             200..1351
                     /label=LacI + LVA
     CDS             200..1351
                     /label=BBa_K2180009
     misc_feature    1360..1365
                     /label=BmtI
     misc_feature    1366..1406
                     /label=Transcription terminator
     misc_feature    1415..1460
                     /label=Transcription terminator'
     misc_feature    1461..1466
                     /label=AvrII
     terminator      1360..1466
                     /label=BBa_K2180008
     misc_feature    1..1466
                     /label=BBa_K2180004
     misc_feature    1484..1489
                     /label=SalI restriction site
     promoter        1497..1653
                     /label=PlepA
     misc_feature    1654..1661
                     /label=PacI restriction site
     misc_feature    1662..1682
                     /label=LacI Operator O1
     misc_feature    1683..1703
                     /label=LacI Operator O1
     misc_feature    1704..1709
                     /label=SacII restriction site
     RBS             1710..1720
                     /label=RBS
     misc_feature    1728..1732
                     /label=NdeI
     promoter        1475..1732
                     /label=BBa_K2180006
     CDS             1744..2445
                     /label=Pigment
     misc_feature    1744..1746
                     /label=start
     misc_feature    1741..1746
                     /label=Nde I restriction site
     misc_feature    2446..2451
                     /label=Avr II restriction site
     misc_feature    2407..2439
                     /label=LVA tail
     CDS             1741..2451
                     /label=BBa_K2180005
     misc_feature    2460..2465
                     /label=BmtI
     misc_feature    2466..2506
                     /label=Transcription terminator
     misc_feature    2515..2560
                     /label=Transcription terminator'
     misc_feature    2561..2566
                     /label=AvrII
     terminator      2460..2566
                     /label=BBa_K2180008
     misc_feature    1475..2566
                     /label=BBa_K2180018
ORIGIN
        1 ggatcccaag cttggcaaaa atcagaccag acaaaagcgg caaatgaata agcggaacgg
       61 ggaaggattt gcggtcaagt ccttcccttc cgcacgtatc aattcgcaag cttttccttt
      121 ataatagaat gaatgaatcg attgataatg ataatcatta tcaacatgta aaggaggtgt
      181 ctttatccca tggtactaga tggtaaacgt gaaaccagtg acattgtacg atgttgctga
      241 gtatgcaggc gtcagctatc aaactgtaag tcgagtagtt aaccaagcca gccatgtgag
      301 cgcaaaaacg cgcgagaagg tggaagcggc gatggctgag ttaaactata taccaaatag
      361 agtcgctcag caattagccg gcaaacagtc cctgttgatc ggtgtagcga cctcctctct
      421 ggcattgcat gctccgtctc agatcgtggc agcaattaag tcacgtgctg atcaattagg
      481 agcttctgtt gttgtcagca tggtggaaag gagcggagtc gaggcgtgca aagcggcggt
      541 tcataaccta ctagcccagc gcgtgtctgg gcttattata aattaccccc tggatgatca
      601 agatgcaatc gcggttgaag cagcttgtac gaacgtccca gccttgttcc tcgacgtcag
      661 tgaccagacg ccgattaatt ccatcatctt tagccacgag gacggaactc gcctcggcgt
      721 ggagcatctt gtcgcgttgg gacaccagca gattgctcta ctcgcggggc cgttaagttc
      781 tgtatcagca cgtttaagac tcgcaggctg gcataaatat ttgaccagaa atcaaattca
      841 accaattgct gaacgcgaag gagattggtc ggcaatgagc ggcttccagc agacgatgca
      901 aatgctgaat gaaggtattg ttcctaccgc aatgttagtg gccaacgacc aaatggcgct
      961 cggtgcgatg cgtgcaatca ccgaatcagg cttgcgtgtg ggggctgata tctccgtggt
     1021 cgggtatgac gatactgaag attcaagctg ttatattccg cctttgacaa ctataaaaca
     1081 agattttaga ctgctaggcc agacatcagt agatcgactg ctgcaacttt cccaggggca
     1141 agcggttaaa ggaaaccaac tgttaccggt atctctcgtc aaacgcaaga caacattagc
     1201 cccgaatacc caaacagcat ccccgcgcgc tctcgctgac tcacttatgc agcttgcccg
     1261 gcaggtcagt agactggaat cagggcaggc agctaatgac gaaaattatg cccttgtagc
     1321 ctaatgactt taataatgct gatgcaggtc atactagagg ctagctcaca ctggctcacc
     1381 ttcgggtggg cctttctgcg tttatatact agagagagaa tataaaaagc cagattatta
     1441 atccggcttt tttattattt cctaggtact agagccaagc ttggtcgacg tcgaaaagtc
     1501 aatgtatgaa tggatacggg atatgaatca ataagtacgt gaaagagaaa agcaacccag
     1561 atatgatagg gaacttttct ctttcttgtt ttacattgaa tctttacaat cctattgata
     1621 taatctaagc tagtgtattt tgcgtttaat agtttaatta aaattgtgag cggataacaa
     1681 ttaattgtga gcggataaca attccgcgga aaggaggtgt ctttatcata tgtactagag
     1741 catatgtccg taattgctaa acaaatgacc tacaaagttt acatgagcgg cacagttaac
     1801 ggtcactatt tcgaagtgga gggagatgga aaaggaaaac catacgaagg tgagcagacc
     1861 gttaaactca ccgtaacgaa gggtggacca cttccgtttg cctgggatat tctaagtccg
     1921 caatgccaat acgggtcaat tcctttcact aaataccctg aggacatccc ggattacgtt
     1981 aagcaaagtt tcccggaagg atacacatgg gaacggatta tgaactttga agacggtgcg
     2041 gtctgcacag tctcgaatga tagtagcatc cagggtaatt gttttattta tcacgtcaag
     2101 ttctcaggcc ttaacttccc tccgaacggg cccgtgatgc agaaaaagac gcaaggctgg
     2161 gagccaaaca cggaacgcct cttcgcaagg gatggaatgt tgttaggcaa taactttatg
     2221 gcgctcaaac tggaaggcgg tggacattat ctgtgcgaat ttaaaactac atataaagca
     2281 aaaaagccgg tcaagatgcc gggataccat tatgtagatc gtaaactgga cgtgaccaat
     2341 cataataagg actatacatc agtggaacag tgcgaaatct caatcgctcg taagccagtc
     2401 gtggccgctg caaacgacga aaactacgct ttagtagctt aataacctag gtactagagg
     2461 ctagctcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa
     2521 tataaaaagc cagattatta atccggcttt tttattattt cctagg
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