BBa_K2180018 1 BBa_K2180018 Promoter PlepA + Operator LacI + Blue Pigment + Double terminator 2016-10-13T11:00:00Z 2016-10-29T06:47:03Z Lac I Pig Lac I Pig false false _2651_ 32196 32196 9 false Lac I Pig false Wilhelm Vaysse-Zinkh??fer component2531007 1 BBa_K2180006 component2531013 1 BBa_K2180005 component2531018 1 BBa_K2180008 annotation2531013 1 BBa_K2180005 range2531013 1 267 977 annotation2531018 1 BBa_K2180008 range2531018 1 986 1092 annotation2531007 1 BBa_K2180006 range2531007 1 1 258 BBa_K2180009 1 BBa_K2180009 LacI-LVA for B. subtilis 2016-08-04T11:00:00Z 2016-10-19T08:26:00Z This is the assembly of our basic parts BBa_K2180004 , BBa_K2180007 without the Fur Box and BBa_K2180008 We delete the Fur Box in order to use it like a normal promoter. The NcoI restriction site is overlapping parts BBa_K2180004 and BBa_K2180007 at the Start codon of LacI + LVA. false false _2651_ 25719 25719 9 false false Sofiane Safi-Stibler annotation2521118 1 LacI + LVA range2521118 1 1 1152 BBa_K2180012 1 BBa_K2180012 Promoter PlepA +FUR regulatory sequence + RBS + LacI-LVA + Terminator + Pigment + RBS + Operateur La 2016-08-21T11:00:00Z 2016-10-29T05:57:12Z false false _2651_ 32196 25719 9 false false Sofiane Safi-Stibler component2531208 1 BBa_K2180004 component2531229 1 BBa_K2180018 annotation2531229 1 BBa_K2180018 range2531229 1 1475 2566 annotation2531208 1 BBa_K2180004 range2531208 1 1 1466 BBa_K2180008 1 BBa_K2180008 Terminator 2016-08-04T11:00:00Z 2016-10-13T10:53:37Z This is a double terminator we use in our project for the pigment production. He is made of a transcriptional terminator in forward and reverse direction. There is a BmtI restriction site before the first terminator (forward) and an AvrII restriction site after the second terminator (reverse). false false _2651_ 25719 25719 9 false false Sofiane Safi-Stibler annotation2481416 1 AvrII range2481416 1 102 107 annotation2481414 1 Transcription terminator range2481414 1 7 47 annotation2481413 1 BmtI range2481413 1 1 6 annotation2481415 1 Transcription terminator' range2481415 1 56 101 BBa_K2180006 1 BBa_K2180006 PLacO - Promoter LacO for B. subtilis 2016-08-04T11:00:00Z 2016-10-13T10:58:17Z The part is made of a previous IGEM part (add part) This part is made of PlepA promoter, two LacI binding sites and a RBS. There is a PacI restriction site before LacI binding sites and a SacII restriction site after them. They allows us to remplace the LacI binding sites by another operating sequence. A SalI restriction sites is presents before the PlepA sequence that may be used to insert the whole sequence. We use this part in our project to promote the pigment expression under the control of LacI. false false _2651_ 25719 25719 9 false false Sofiane Safi-Stibler annotation2481402 1 PacI restriction site range2481402 1 180 187 annotation2481405 1 SacII restriction site range2481405 1 230 235 annotation2481418 1 NdeI range2481418 1 254 258 annotation2481401 1 PlepA range2481401 1 23 179 annotation2481400 1 SalI restriction site range2481400 1 10 15 annotation2481404 1 LacI Operator O1 range2481404 1 209 229 annotation2481403 1 LacI Operator O1 range2481403 1 188 208 annotation2481406 1 RBS range2481406 1 236 246 BBa_K2180007 1 BBa_K2180007 PFur - Promoter Fur for B. subtilis 2016-08-04T11:00:00Z 2016-10-13T10:58:33Z This part is based on a previous IGEM part (add part). We add the Fur Box site we found (add site) This part is made of PleiaG promoter, a fur (Ferric Uptake Regulator) binding sites and a RBS. There is a ClaI restriction site before the Fur Box (Fur Binding Site) and a PscI restriction site after. They allows us to remplace Fur Box by another operating sequence. A BamHI restriction sites is present before the PliaG sequence that may be used to insert the whole sequence. We use this part in our project to promote the LacI + LVA expression under the control of Fur. false false _2651_ 25719 25719 9 false We had to check the compatibility of the promoter with the Fur Box. false Sofiane Safi-Stibler annotation2481417 1 NcoI range2481417 1 188 193 annotation2481410 1 Fur Box range2481410 1 143 163 annotation2481407 1 BamHI range2481407 1 1 6 annotation2481411 1 PscI range2481411 1 164 169 annotation2481408 1 PliaG range2481408 1 16 136 annotation2481409 1 ClaI range2481409 1 137 142 annotation2481412 1 RBS range2481412 1 170 180 BBa_K2180004 1 BBa_K2180004 Promoter +FUR regulatory sequence + RBS + LacI-LVA + Terminator 2016-08-04T11:00:00Z 2016-10-29T05:30:41Z The sequence is based on a previous IGEM part (add part) coding for LacI + LVA for E. coli Coding sequence of LacI protein + LVA (rapid degradation tail) wich improve the switch time and make it more sensitive. false false _2651_ 32196 25719 9 false We had to improve the codon usage for B. subtilis false Sofiane Safi-Stibler component2521317 1 BBa_K2180007 component2521319 1 BBa_K2180009 component2521324 1 BBa_K2180008 annotation2521319 1 BBa_K2180009 range2521319 1 200 1351 annotation2521324 1 BBa_K2180008 range2521324 1 1360 1466 annotation2521317 1 BBa_K2180007 range2521317 1 1 193 BBa_K2180005 1 BBa_K2180005 Pigment Blue for B. subtilis with a LVA tail and restriction site (Blue Pigment) 2016-08-04T11:00:00Z 2016-10-21T08:21:50Z This part come from a previous IGEM part (add part) This sequence is coding for a blue pigment we use in our detection system as a reporter. false false _2651_ 32196 25719 9 false We had to improve the codon usage for B. subtilis false Sofiane Safi-Stibler annotation2527142 1 Avr II restriction site range2527142 1 706 711 annotation2527143 1 LVA tail range2527143 1 667 699 annotation2527141 1 Nde I restriction site range2527141 1 1 6 annotation2527137 1 start range2527137 1 4 6 annotation2481399 1 Pigment range2481399 1 4 705 BBa_K2180006_sequence 1 ccaagcttggtcgacgtcgaaaagtcaatgtatgaatggatacgggatatgaatcaataagtacgtgaaagagaaaagcaacccagatatgatagggaacttttctctttcttgttttacattgaatctttacaatcctattgatataatctaagctagtgtattttgcgtttaatagtttaattaaaattgtgagcggataacaattaattgtgagcggataacaattccgcggaaaggaggtgtctttatcatatg BBa_K2180004_sequence 1 ggatcccaagcttggcaaaaatcagaccagacaaaagcggcaaatgaataagcggaacggggaaggatttgcggtcaagtccttcccttccgcacgtatcaattcgcaagcttttcctttataatagaatgaatgaatcgattgataatgataatcattatcaacatgtaaaggaggtgtctttatcccatggtactagatggtaaacgtgaaaccagtgacattgtacgatgttgctgagtatgcaggcgtcagctatcaaactgtaagtcgagtagttaaccaagccagccatgtgagcgcaaaaacgcgcgagaaggtggaagcggcgatggctgagttaaactatataccaaatagagtcgctcagcaattagccggcaaacagtccctgttgatcggtgtagcgacctcctctctggcattgcatgctccgtctcagatcgtggcagcaattaagtcacgtgctgatcaattaggagcttctgttgttgtcagcatggtggaaaggagcggagtcgaggcgtgcaaagcggcggttcataacctactagcccagcgcgtgtctgggcttattataaattaccccctggatgatcaagatgcaatcgcggttgaagcagcttgtacgaacgtcccagccttgttcctcgacgtcagtgaccagacgccgattaattccatcatctttagccacgaggacggaactcgcctcggcgtggagcatcttgtcgcgttgggacaccagcagattgctctactcgcggggccgttaagttctgtatcagcacgtttaagactcgcaggctggcataaatatttgaccagaaatcaaattcaaccaattgctgaacgcgaaggagattggtcggcaatgagcggcttccagcagacgatgcaaatgctgaatgaaggtattgttcctaccgcaatgttagtggccaacgaccaaatggcgctcggtgcgatgcgtgcaatcaccgaatcaggcttgcgtgtgggggctgatatctccgtggtcgggtatgacgatactgaagattcaagctgttatattccgcctttgacaactataaaacaagattttagactgctaggccagacatcagtagatcgactgctgcaactttcccaggggcaagcggttaaaggaaaccaactgttaccggtatctctcgtcaaacgcaagacaacattagccccgaatacccaaacagcatccccgcgcgctctcgctgactcacttatgcagcttgcccggcaggtcagtagactggaatcagggcaggcagctaatgacgaaaattatgcccttgtagcctaatgactttaataatgctgatgcaggtcatactagaggctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagg BBa_K2180009_sequence 1 atggtaaacgtgaaaccagtgacattgtacgatgttgctgagtatgcaggcgtcagctatcaaactgtaagtcgagtagttaaccaagccagccatgtgagcgcaaaaacgcgcgagaaggtggaagcggcgatggctgagttaaactatataccaaatagagtcgctcagcaattagccggcaaacagtccctgttgatcggtgtagcgacctcctctctggcattgcatgctccgtctcagatcgtggcagcaattaagtcacgtgctgatcaattaggagcttctgttgttgtcagcatggtggaaaggagcggagtcgaggcgtgcaaagcggcggttcataacctactagcccagcgcgtgtctgggcttattataaattaccccctggatgatcaagatgcaatcgcggttgaagcagcttgtacgaacgtcccagccttgttcctcgacgtcagtgaccagacgccgattaattccatcatctttagccacgaggacggaactcgcctcggcgtggagcatcttgtcgcgttgggacaccagcagattgctctactcgcggggccgttaagttctgtatcagcacgtttaagactcgcaggctggcataaatatttgaccagaaatcaaattcaaccaattgctgaacgcgaaggagattggtcggcaatgagcggcttccagcagacgatgcaaatgctgaatgaaggtattgttcctaccgcaatgttagtggccaacgaccaaatggcgctcggtgcgatgcgtgcaatcaccgaatcaggcttgcgtgtgggggctgatatctccgtggtcgggtatgacgatactgaagattcaagctgttatattccgcctttgacaactataaaacaagattttagactgctaggccagacatcagtagatcgactgctgcaactttcccaggggcaagcggttaaaggaaaccaactgttaccggtatctctcgtcaaacgcaagacaacattagccccgaatacccaaacagcatccccgcgcgctctcgctgactcacttatgcagcttgcccggcaggtcagtagactggaatcagggcaggcagctaatgacgaaaattatgcccttgtagcctaatgactttaataatgctgatgcaggtca BBa_K2180018_sequence 1 ccaagcttggtcgacgtcgaaaagtcaatgtatgaatggatacgggatatgaatcaataagtacgtgaaagagaaaagcaacccagatatgatagggaacttttctctttcttgttttacattgaatctttacaatcctattgatataatctaagctagtgtattttgcgtttaatagtttaattaaaattgtgagcggataacaattaattgtgagcggataacaattccgcggaaaggaggtgtctttatcatatgtactagagcatatgtccgtaattgctaaacaaatgacctacaaagtttacatgagcggcacagttaacggtcactatttcgaagtggagggagatggaaaaggaaaaccatacgaaggtgagcagaccgttaaactcaccgtaacgaagggtggaccacttccgtttgcctgggatattctaagtccgcaatgccaatacgggtcaattcctttcactaaataccctgaggacatcccggattacgttaagcaaagtttcccggaaggatacacatgggaacggattatgaactttgaagacggtgcggtctgcacagtctcgaatgatagtagcatccagggtaattgttttatttatcacgtcaagttctcaggccttaacttccctccgaacgggcccgtgatgcagaaaaagacgcaaggctgggagccaaacacggaacgcctcttcgcaagggatggaatgttgttaggcaataactttatggcgctcaaactggaaggcggtggacattatctgtgcgaatttaaaactacatataaagcaaaaaagccggtcaagatgccgggataccattatgtagatcgtaaactggacgtgaccaatcataataaggactatacatcagtggaacagtgcgaaatctcaatcgctcgtaagccagtcgtggccgctgcaaacgacgaaaactacgctttagtagcttaataacctaggtactagaggctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagg BBa_K2180005_sequence 1 catatgtccgtaattgctaaacaaatgacctacaaagtttacatgagcggcacagttaacggtcactatttcgaagtggagggagatggaaaaggaaaaccatacgaaggtgagcagaccgttaaactcaccgtaacgaagggtggaccacttccgtttgcctgggatattctaagtccgcaatgccaatacgggtcaattcctttcactaaataccctgaggacatcccggattacgttaagcaaagtttcccggaaggatacacatgggaacggattatgaactttgaagacggtgcggtctgcacagtctcgaatgatagtagcatccagggtaattgttttatttatcacgtcaagttctcaggccttaacttccctccgaacgggcccgtgatgcagaaaaagacgcaaggctgggagccaaacacggaacgcctcttcgcaagggatggaatgttgttaggcaataactttatggcgctcaaactggaaggcggtggacattatctgtgcgaatttaaaactacatataaagcaaaaaagccggtcaagatgccgggataccattatgtagatcgtaaactggacgtgaccaatcataataaggactatacatcagtggaacagtgcgaaatctcaatcgctcgtaagccagtcgtggccgctgcaaacgacgaaaactacgctttagtagcttaataacctagg BBa_K2180007_sequence 1 ggatcccaagcttggcaaaaatcagaccagacaaaagcggcaaatgaataagcggaacggggaaggatttgcggtcaagtccttcccttccgcacgtatcaattcgcaagcttttcctttataatagaatgaatgaatcgattgataatgataatcattatcaacatgtaaaggaggtgtctttatcccatgg BBa_K2180012_sequence 1 ggatcccaagcttggcaaaaatcagaccagacaaaagcggcaaatgaataagcggaacggggaaggatttgcggtcaagtccttcccttccgcacgtatcaattcgcaagcttttcctttataatagaatgaatgaatcgattgataatgataatcattatcaacatgtaaaggaggtgtctttatcccatggtactagatggtaaacgtgaaaccagtgacattgtacgatgttgctgagtatgcaggcgtcagctatcaaactgtaagtcgagtagttaaccaagccagccatgtgagcgcaaaaacgcgcgagaaggtggaagcggcgatggctgagttaaactatataccaaatagagtcgctcagcaattagccggcaaacagtccctgttgatcggtgtagcgacctcctctctggcattgcatgctccgtctcagatcgtggcagcaattaagtcacgtgctgatcaattaggagcttctgttgttgtcagcatggtggaaaggagcggagtcgaggcgtgcaaagcggcggttcataacctactagcccagcgcgtgtctgggcttattataaattaccccctggatgatcaagatgcaatcgcggttgaagcagcttgtacgaacgtcccagccttgttcctcgacgtcagtgaccagacgccgattaattccatcatctttagccacgaggacggaactcgcctcggcgtggagcatcttgtcgcgttgggacaccagcagattgctctactcgcggggccgttaagttctgtatcagcacgtttaagactcgcaggctggcataaatatttgaccagaaatcaaattcaaccaattgctgaacgcgaaggagattggtcggcaatgagcggcttccagcagacgatgcaaatgctgaatgaaggtattgttcctaccgcaatgttagtggccaacgaccaaatggcgctcggtgcgatgcgtgcaatcaccgaatcaggcttgcgtgtgggggctgatatctccgtggtcgggtatgacgatactgaagattcaagctgttatattccgcctttgacaactataaaacaagattttagactgctaggccagacatcagtagatcgactgctgcaactttcccaggggcaagcggttaaaggaaaccaactgttaccggtatctctcgtcaaacgcaagacaacattagccccgaatacccaaacagcatccccgcgcgctctcgctgactcacttatgcagcttgcccggcaggtcagtagactggaatcagggcaggcagctaatgacgaaaattatgcccttgtagcctaatgactttaataatgctgatgcaggtcatactagaggctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctaggtactagagccaagcttggtcgacgtcgaaaagtcaatgtatgaatggatacgggatatgaatcaataagtacgtgaaagagaaaagcaacccagatatgatagggaacttttctctttcttgttttacattgaatctttacaatcctattgatataatctaagctagtgtattttgcgtttaatagtttaattaaaattgtgagcggataacaattaattgtgagcggataacaattccgcggaaaggaggtgtctttatcatatgtactagagcatatgtccgtaattgctaaacaaatgacctacaaagtttacatgagcggcacagttaacggtcactatttcgaagtggagggagatggaaaaggaaaaccatacgaaggtgagcagaccgttaaactcaccgtaacgaagggtggaccacttccgtttgcctgggatattctaagtccgcaatgccaatacgggtcaattcctttcactaaataccctgaggacatcccggattacgttaagcaaagtttcccggaaggatacacatgggaacggattatgaactttgaagacggtgcggtctgcacagtctcgaatgatagtagcatccagggtaattgttttatttatcacgtcaagttctcaggccttaacttccctccgaacgggcccgtgatgcagaaaaagacgcaaggctgggagccaaacacggaacgcctcttcgcaagggatggaatgttgttaggcaataactttatggcgctcaaactggaaggcggtggacattatctgtgcgaatttaaaactacatataaagcaaaaaagccggtcaagatgccgggataccattatgtagatcgtaaactggacgtgaccaatcataataaggactatacatcagtggaacagtgcgaaatctcaatcgctcgtaagccagtcgtggccgctgcaaacgacgaaaactacgctttagtagcttaataacctaggtactagaggctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagg BBa_K2180008_sequence 1 gctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z