Promoter PlepA +FUR regulatory sequence + RBS + LacI-LVA + Terminator + Pigment + RBS + Operateur La
Promoter +FUR regulatory sequence + RBS + LacI-LVA + Terminator
The sequence is based on a previous IGEM part (add part) coding for LacI + LVA for E. coli
Coding sequence of LacI protein + LVA (rapid degradation tail) wich improve the switch time and make it more sensitive.
We had to improve the codon usage for B. subtilis
Promoter PlepA + Operator LacI + Blue Pigment + Double terminator
This part is based on a previous IGEM part (add part). We add the Fur Box site we found (add site)
This part is made of PleiaG promoter, a fur (Ferric Uptake Regulator) binding sites and a RBS. There is a ClaI restriction site before the Fur Box (Fur Binding Site) and a PscI restriction site after. They allows us to remplace Fur Box by another operating sequence. A BamHI restriction sites is present before the PliaG sequence that may be used to insert the whole sequence. We use this part in our project to promote the LacI + LVA expression under the control of Fur.
We had to check the compatibility of the promoter with the Fur Box.
This is a double terminator we use in our project for the pigment production. He is made of a transcriptional terminator in forward and reverse direction. There is a BmtI restriction site before the first terminator (forward) and an AvrII restriction site after the second terminator (reverse).
This is the assembly of our basic parts BBa_K2180004 , BBa_K2180007 without the Fur Box and BBa_K2180008 We delete the Fur Box in order to use it like a normal promoter. The NcoI restriction site is overlapping parts BBa_K2180004 and BBa_K2180007 at the Start codon of LacI + LVA.
Pigment Blue for B. subtilis with a LVA tail and restriction site (Blue Pigment)
This part come from a previous IGEM part (add part)
This sequence is coding for a blue pigment we use in our detection system as a reporter.
The part is made of a previous IGEM part (add part)
This part is made of PlepA promoter, two LacI binding sites and a RBS. There is a PacI restriction site before LacI binding sites and a SacII restriction site after them. They allows us to remplace the LacI binding sites by another operating sequence. A SalI restriction sites is presents before the PlepA sequence that may be used to insert the whole sequence. We use this part in our project to promote the pigment expression under the control of LacI.
Conversion of the iGEM parts registry to SBOL2.1