BBa_K2180012
type: ComponentDefinition
persistent identity: BBa K2180012
display id: BBa_K2180012
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter PlepA +FUR regulatory sequence + RBS + LacI-LVA + Terminator + Pigment + RBS + Operateur La
created: 2016-08-21T11:00:00Z
modified: 2016-10-29T05:57:12Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _2651_
m user id: 32196
owner id: 25719
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Sofiane Safi-Stibler
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K2180018
component: BBa_K2180004
sequence annotation: BBa_K2180004
sequence annotation: BBa_K2180018
sequence: https://synbiohub.org/public/igem/BBa_K2180012_sequence/1

BBa_K2180018
type: Component
persistent identity: component2531229
display id: component2531229
version: 1
top level: BBa_K2180012
definition: BBa_K2180018
access: public

BBa_K2180004
type: Component
persistent identity: component2531208
display id: component2531208
version: 1
top level: BBa_K2180012
definition: BBa_K2180004
access: public

BBa_K2180004
type: SequenceAnnotation
persistent identity: annotation2531208
display id: annotation2531208
version: 1
top level: BBa_K2180012
location: https://synbiohub.org/public/igem/BBa_K2180012/annotation2531208/range2531208/1
component: BBa_K2180004

https://synbiohub.org/public/igem/BBa_K2180012/annotation2531208/range2531208/1
type: Range
persistent identity: range2531208
display id: range2531208
version: 1
top level: BBa_K2180012
direction: direction/reverse
start: 1
end: 1466
orientation: inline

BBa_K2180018
type: SequenceAnnotation
persistent identity: annotation2531229
display id: annotation2531229
version: 1
top level: BBa_K2180012
location: https://synbiohub.org/public/igem/BBa_K2180012/annotation2531229/range2531229/1
component: BBa_K2180018

https://synbiohub.org/public/igem/BBa_K2180012/annotation2531229/range2531229/1
type: Range
persistent identity: range2531229
display id: range2531229
version: 1
top level: BBa_K2180012
direction: direction/reverse
start: 1475
end: 2566
orientation: inline

BBa_K2180004
type: ComponentDefinition
persistent identity: BBa K2180004
display id: BBa_K2180004
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter +FUR regulatory sequence + RBS + LacI-LVA + Terminator
created: 2016-08-04T11:00:00Z
modified: 2016-10-29T05:30:41Z
mutable provenance: The sequence is based on a previous IGEM part (add part) coding for LacI + LVA for E. coli
owned by: james
owned by: myers
mutable description: Coding sequence of LacI protein + LVA (rapid degradation tail) wich improve the switch time and make it more sensitive.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2651_
m user id: 32196
owner id: 25719
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We had to improve the codon usage for B. subtilis
star: false
creator: Sofiane Safi-Stibler
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K2180007
component: BBa_K2180008
component: BBa_K2180009
sequence annotation: BBa_K2180007
sequence annotation: BBa_K2180009
sequence annotation: BBa_K2180008
sequence: https://synbiohub.org/public/igem/BBa_K2180004_sequence/1

BBa_K2180007
type: Component
persistent identity: component2521317
display id: component2521317
version: 1
top level: BBa_K2180004
definition: BBa_K2180007
access: public

BBa_K2180008
type: Component
persistent identity: component2521324
display id: component2521324
version: 1
top level: BBa_K2180004
definition: BBa_K2180008
access: public

BBa_K2180009
type: Component
persistent identity: component2521319
display id: component2521319
version: 1
top level: BBa_K2180004
definition: BBa_K2180009
access: public

BBa_K2180007
type: SequenceAnnotation
persistent identity: annotation2521317
display id: annotation2521317
version: 1
top level: BBa_K2180004
location: https://synbiohub.org/public/igem/BBa_K2180004/annotation2521317/range2521317/1
component: BBa_K2180007

https://synbiohub.org/public/igem/BBa_K2180004/annotation2521317/range2521317/1
type: Range
persistent identity: range2521317
display id: range2521317
version: 1
top level: BBa_K2180004
direction: direction/reverse
start: 1
end: 193
orientation: inline

BBa_K2180009
type: SequenceAnnotation
persistent identity: annotation2521319
display id: annotation2521319
version: 1
top level: BBa_K2180004
location: https://synbiohub.org/public/igem/BBa_K2180004/annotation2521319/range2521319/1
component: BBa_K2180009

https://synbiohub.org/public/igem/BBa_K2180004/annotation2521319/range2521319/1
type: Range
persistent identity: range2521319
display id: range2521319
version: 1
top level: BBa_K2180004
direction: direction/reverse
start: 200
end: 1351
orientation: inline

BBa_K2180008
type: SequenceAnnotation
persistent identity: annotation2521324
display id: annotation2521324
version: 1
top level: BBa_K2180004
location: https://synbiohub.org/public/igem/BBa_K2180004/annotation2521324/range2521324/1
component: BBa_K2180008

https://synbiohub.org/public/igem/BBa_K2180004/annotation2521324/range2521324/1
type: Range
persistent identity: range2521324
display id: range2521324
version: 1
top level: BBa_K2180004
direction: direction/reverse
start: 1360
end: 1466
orientation: inline

BBa_K2180018
type: ComponentDefinition
persistent identity: BBa K2180018
display id: BBa_K2180018
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter PlepA + Operator LacI + Blue Pigment + Double terminator
created: 2016-10-13T11:00:00Z
modified: 2016-10-29T06:47:03Z
mutable provenance: Lac I Pig
owned by: james
owned by: myers
mutable description: Lac I Pig
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2651_
m user id: 32196
owner id: 32196
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Lac I Pig
star: false
creator: Wilhelm Vaysse-Zinkh??fer
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K2180006
component: BBa_K2180008
component: BBa_K2180005
sequence annotation: BBa_K2180006
sequence annotation: BBa_K2180005
sequence annotation: BBa_K2180008
sequence: https://synbiohub.org/public/igem/BBa_K2180018_sequence/1

BBa_K2180006
type: Component
persistent identity: component2531007
display id: component2531007
version: 1
top level: BBa_K2180018
definition: BBa_K2180006
access: public

BBa_K2180008
type: Component
persistent identity: component2531018
display id: component2531018
version: 1
top level: BBa_K2180018
definition: BBa_K2180008
access: public

BBa_K2180005
type: Component
persistent identity: component2531013
display id: component2531013
version: 1
top level: BBa_K2180018
definition: BBa_K2180005
access: public

BBa_K2180006
type: SequenceAnnotation
persistent identity: annotation2531007
display id: annotation2531007
version: 1
top level: BBa_K2180018
location: https://synbiohub.org/public/igem/BBa_K2180018/annotation2531007/range2531007/1
component: BBa_K2180006

https://synbiohub.org/public/igem/BBa_K2180018/annotation2531007/range2531007/1
type: Range
persistent identity: range2531007
display id: range2531007
version: 1
top level: BBa_K2180018
direction: direction/reverse
start: 1
end: 258
orientation: inline

BBa_K2180005
type: SequenceAnnotation
persistent identity: annotation2531013
display id: annotation2531013
version: 1
top level: BBa_K2180018
location: https://synbiohub.org/public/igem/BBa_K2180018/annotation2531013/range2531013/1
component: BBa_K2180005

https://synbiohub.org/public/igem/BBa_K2180018/annotation2531013/range2531013/1
type: Range
persistent identity: range2531013
display id: range2531013
version: 1
top level: BBa_K2180018
direction: direction/reverse
start: 267
end: 977
orientation: inline

BBa_K2180008
type: SequenceAnnotation
persistent identity: annotation2531018
display id: annotation2531018
version: 1
top level: BBa_K2180018
location: https://synbiohub.org/public/igem/BBa_K2180018/annotation2531018/range2531018/1
component: BBa_K2180008

https://synbiohub.org/public/igem/BBa_K2180018/annotation2531018/range2531018/1
type: Range
persistent identity: range2531018
display id: range2531018
version: 1
top level: BBa_K2180018
direction: direction/reverse
start: 986
end: 1092
orientation: inline

BBa_K2180007
type: ComponentDefinition
persistent identity: BBa K2180007
display id: BBa_K2180007
version: 1
was generated by: iGEM to SBOL conversion
description: PFur - Promoter Fur for B. subtilis
created: 2016-08-04T11:00:00Z
modified: 2016-10-13T10:58:33Z
mutable provenance: This part is based on a previous IGEM part (add part). We add the Fur Box site we found (add site)
owned by: james
owned by: myers
mutable description: This part is made of PleiaG promoter, a fur (Ferric Uptake Regulator) binding sites and a RBS. There is a ClaI restriction site before the Fur Box (Fur Binding Site) and a PscI restriction site after. They allows us to remplace Fur Box by another operating sequence. A BamHI restriction sites is present before the PliaG sequence that may be used to insert the whole sequence. We use this part in our project to promote the LacI + LVA expression under the control of Fur.
discontinued: false
dominant: false
experience: experience/None
group u list: _2651_
m user id: 25719
owner id: 25719
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We had to check the compatibility of the promoter with the Fur Box.
star: false
creator: Sofiane Safi-Stibler
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BamHI
sequence annotation: PliaG
sequence annotation: ClaI
sequence annotation: Fur Box
sequence annotation: PscI
sequence annotation: RBS
sequence annotation: NcoI
sequence: https://synbiohub.org/public/igem/BBa_K2180007_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation2481407
display id: annotation2481407
version: 1
top level: BBa_K2180007
location: https://synbiohub.org/public/igem/BBa_K2180007/annotation2481407/range2481407/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K2180007/annotation2481407/range2481407/1
type: Range
persistent identity: range2481407
display id: range2481407
version: 1
top level: BBa_K2180007
direction: direction/forward
start: 1
end: 6
orientation: inline

PliaG
type: SequenceAnnotation
persistent identity: annotation2481408
display id: annotation2481408
version: 1
top level: BBa_K2180007
location: https://synbiohub.org/public/igem/BBa_K2180007/annotation2481408/range2481408/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K2180007/annotation2481408/range2481408/1
type: Range
persistent identity: range2481408
display id: range2481408
version: 1
top level: BBa_K2180007
direction: direction/forward
start: 16
end: 136
orientation: inline

ClaI
type: SequenceAnnotation
persistent identity: annotation2481409
display id: annotation2481409
version: 1
top level: BBa_K2180007
location: https://synbiohub.org/public/igem/BBa_K2180007/annotation2481409/range2481409/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K2180007/annotation2481409/range2481409/1
type: Range
persistent identity: range2481409
display id: range2481409
version: 1
top level: BBa_K2180007
direction: direction/forward
start: 137
end: 142
orientation: inline

Fur Box
type: SequenceAnnotation
persistent identity: annotation2481410
display id: annotation2481410
version: 1
top level: BBa_K2180007
location: https://synbiohub.org/public/igem/BBa_K2180007/annotation2481410/range2481410/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K2180007/annotation2481410/range2481410/1
type: Range
persistent identity: range2481410
display id: range2481410
version: 1
top level: BBa_K2180007
direction: direction/forward
start: 143
end: 163
orientation: inline

PscI
type: SequenceAnnotation
persistent identity: annotation2481411
display id: annotation2481411
version: 1
top level: BBa_K2180007
location: https://synbiohub.org/public/igem/BBa_K2180007/annotation2481411/range2481411/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K2180007/annotation2481411/range2481411/1
type: Range
persistent identity: range2481411
display id: range2481411
version: 1
top level: BBa_K2180007
direction: direction/forward
start: 164
end: 169
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2481412
display id: annotation2481412
version: 1
top level: BBa_K2180007
location: https://synbiohub.org/public/igem/BBa_K2180007/annotation2481412/range2481412/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K2180007/annotation2481412/range2481412/1
type: Range
persistent identity: range2481412
display id: range2481412
version: 1
top level: BBa_K2180007
direction: direction/forward
start: 170
end: 180
orientation: inline

NcoI
type: SequenceAnnotation
persistent identity: annotation2481417
display id: annotation2481417
version: 1
top level: BBa_K2180007
location: https://synbiohub.org/public/igem/BBa_K2180007/annotation2481417/range2481417/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K2180007/annotation2481417/range2481417/1
type: Range
persistent identity: range2481417
display id: range2481417
version: 1
top level: BBa_K2180007
direction: direction/forward
start: 188
end: 193
orientation: inline

BBa_K2180008
type: ComponentDefinition
persistent identity: BBa K2180008
display id: BBa_K2180008
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator
created: 2016-08-04T11:00:00Z
modified: 2016-10-13T10:53:37Z
owned by: james
owned by: myers
mutable description: This is a double terminator we use in our project for the pigment production. He is made of a transcriptional terminator in forward and reverse direction. There is a BmtI restriction site before the first terminator (forward) and an AvrII restriction site after the second terminator (reverse).
discontinued: false
dominant: false
experience: experience/None
group u list: _2651_
m user id: 25719
owner id: 25719
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Sofiane Safi-Stibler
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BmtI
sequence annotation: Transcription terminator
sequence annotation: Transcription terminator'
sequence annotation: AvrII
sequence: https://synbiohub.org/public/igem/BBa_K2180008_sequence/1

BmtI
type: SequenceAnnotation
persistent identity: annotation2481413
display id: annotation2481413
version: 1
top level: BBa_K2180008
location: https://synbiohub.org/public/igem/BBa_K2180008/annotation2481413/range2481413/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K2180008/annotation2481413/range2481413/1
type: Range
persistent identity: range2481413
display id: range2481413
version: 1
top level: BBa_K2180008
direction: direction/forward
start: 1
end: 6
orientation: inline

Transcription terminator
type: SequenceAnnotation
persistent identity: annotation2481414
display id: annotation2481414
version: 1
top level: BBa_K2180008
location: https://synbiohub.org/public/igem/BBa_K2180008/annotation2481414/range2481414/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K2180008/annotation2481414/range2481414/1
type: Range
persistent identity: range2481414
display id: range2481414
version: 1
top level: BBa_K2180008
direction: direction/forward
start: 7
end: 47
orientation: inline

Transcription terminator'
type: SequenceAnnotation
persistent identity: annotation2481415
display id: annotation2481415
version: 1
top level: BBa_K2180008
location: https://synbiohub.org/public/igem/BBa_K2180008/annotation2481415/range2481415/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K2180008/annotation2481415/range2481415/1
type: Range
persistent identity: range2481415
display id: range2481415
version: 1
top level: BBa_K2180008
direction: direction/forward
start: 56
end: 101
orientation: inline

AvrII
type: SequenceAnnotation
persistent identity: annotation2481416
display id: annotation2481416
version: 1
top level: BBa_K2180008
location: https://synbiohub.org/public/igem/BBa_K2180008/annotation2481416/range2481416/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K2180008/annotation2481416/range2481416/1
type: Range
persistent identity: range2481416
display id: range2481416
version: 1
top level: BBa_K2180008
direction: direction/forward
start: 102
end: 107
orientation: inline

BBa_K2180009
type: ComponentDefinition
persistent identity: BBa K2180009
display id: BBa_K2180009
version: 1
was generated by: iGEM to SBOL conversion
description: LacI-LVA for B. subtilis
created: 2016-08-04T11:00:00Z
modified: 2016-10-19T08:26:00Z
owned by: james
owned by: myers
mutable description: This is the assembly of our basic parts BBa_K2180004 , BBa_K2180007 without the Fur Box and BBa_K2180008 We delete the Fur Box in order to use it like a normal promoter. The NcoI restriction site is overlapping parts BBa_K2180004 and BBa_K2180007 at the Start codon of LacI + LVA.
discontinued: false
dominant: false
experience: experience/None
group u list: _2651_
m user id: 25719
owner id: 25719
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Sofiane Safi-Stibler
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: LacI + LVA
sequence: https://synbiohub.org/public/igem/BBa_K2180009_sequence/1

LacI + LVA
type: SequenceAnnotation
persistent identity: annotation2521118
display id: annotation2521118
version: 1
top level: BBa_K2180009
location: https://synbiohub.org/public/igem/BBa_K2180009/annotation2521118/range2521118/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2180009/annotation2521118/range2521118/1
type: Range
persistent identity: range2521118
display id: range2521118
version: 1
top level: BBa_K2180009
direction: direction/forward
start: 1
end: 1152
orientation: inline

BBa_K2180005
type: ComponentDefinition
persistent identity: BBa K2180005
display id: BBa_K2180005
version: 1
was generated by: iGEM to SBOL conversion
description: Pigment Blue for B. subtilis with a LVA tail and restriction site (Blue Pigment)
created: 2016-08-04T11:00:00Z
modified: 2016-10-21T08:21:50Z
mutable provenance: This part come from a previous IGEM part (add part)
owned by: james
owned by: myers
mutable description: This sequence is coding for a blue pigment we use in our detection system as a reporter.
discontinued: false
dominant: false
experience: experience/None
group u list: _2651_
m user id: 32196
owner id: 25719
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We had to improve the codon usage for B. subtilis
star: false
creator: Sofiane Safi-Stibler
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Nde I restriction site
sequence annotation: Pigment
sequence annotation: start
sequence annotation: LVA tail
sequence annotation: Avr II restriction site
sequence: https://synbiohub.org/public/igem/BBa_K2180005_sequence/1

Nde I restriction site
type: SequenceAnnotation
persistent identity: annotation2527141
display id: annotation2527141
version: 1
top level: BBa_K2180005
location: https://synbiohub.org/public/igem/BBa_K2180005/annotation2527141/range2527141/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K2180005/annotation2527141/range2527141/1
type: Range
persistent identity: range2527141
display id: range2527141
version: 1
top level: BBa_K2180005
direction: direction/forward
start: 1
end: 6
orientation: inline

Pigment
type: SequenceAnnotation
persistent identity: annotation2481399
display id: annotation2481399
version: 1
top level: BBa_K2180005
location: https://synbiohub.org/public/igem/BBa_K2180005/annotation2481399/range2481399/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2180005/annotation2481399/range2481399/1
type: Range
persistent identity: range2481399
display id: range2481399
version: 1
top level: BBa_K2180005
direction: direction/forward
start: 4
end: 705
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2527137
display id: annotation2527137
version: 1
top level: BBa_K2180005
location: https://synbiohub.org/public/igem/BBa_K2180005/annotation2527137/range2527137/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K2180005/annotation2527137/range2527137/1
type: Range
persistent identity: range2527137
display id: range2527137
version: 1
top level: BBa_K2180005
direction: direction/forward
start: 4
end: 6
orientation: inline

LVA tail
type: SequenceAnnotation
persistent identity: annotation2527143
display id: annotation2527143
version: 1
top level: BBa_K2180005
location: https://synbiohub.org/public/igem/BBa_K2180005/annotation2527143/range2527143/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K2180005/annotation2527143/range2527143/1
type: Range
persistent identity: range2527143
display id: range2527143
version: 1
top level: BBa_K2180005
direction: direction/forward
start: 667
end: 699
orientation: inline

Avr II restriction site
type: SequenceAnnotation
persistent identity: annotation2527142
display id: annotation2527142
version: 1
top level: BBa_K2180005
location: https://synbiohub.org/public/igem/BBa_K2180005/annotation2527142/range2527142/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K2180005/annotation2527142/range2527142/1
type: Range
persistent identity: range2527142
display id: range2527142
version: 1
top level: BBa_K2180005
direction: direction/forward
start: 706
end: 711
orientation: inline

BBa_K2180006
type: ComponentDefinition
persistent identity: BBa K2180006
display id: BBa_K2180006
version: 1
was generated by: iGEM to SBOL conversion
description: PLacO - Promoter LacO for B. subtilis
created: 2016-08-04T11:00:00Z
modified: 2016-10-13T10:58:17Z
mutable provenance: The part is made of a previous IGEM part (add part)
owned by: james
owned by: myers
mutable description: This part is made of PlepA promoter, two LacI binding sites and a RBS. There is a PacI restriction site before LacI binding sites and a SacII restriction site after them. They allows us to remplace the LacI binding sites by another operating sequence. A SalI restriction sites is presents before the PlepA sequence that may be used to insert the whole sequence. We use this part in our project to promote the pigment expression under the control of LacI.
discontinued: false
dominant: false
experience: experience/None
group u list: _2651_
m user id: 25719
owner id: 25719
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Sofiane Safi-Stibler
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: SalI restriction site
sequence annotation: PlepA
sequence annotation: PacI restriction site
sequence annotation: LacI Operator O1
sequence annotation: SacII restriction site
sequence annotation: RBS
sequence annotation: NdeI
sequence: https://synbiohub.org/public/igem/BBa_K2180006_sequence/1

SalI restriction site
type: SequenceAnnotation
persistent identity: annotation2481400
display id: annotation2481400
version: 1
top level: BBa_K2180006
location: https://synbiohub.org/public/igem/BBa_K2180006/annotation2481400/range2481400/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K2180006/annotation2481400/range2481400/1
type: Range
persistent identity: range2481400
display id: range2481400
version: 1
top level: BBa_K2180006
direction: direction/forward
start: 10
end: 15
orientation: inline

PlepA
type: SequenceAnnotation
persistent identity: annotation2481401
display id: annotation2481401
version: 1
top level: BBa_K2180006
location: https://synbiohub.org/public/igem/BBa_K2180006/annotation2481401/range2481401/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K2180006/annotation2481401/range2481401/1
type: Range
persistent identity: range2481401
display id: range2481401
version: 1
top level: BBa_K2180006
direction: direction/forward
start: 23
end: 179
orientation: inline

PacI restriction site
type: SequenceAnnotation
persistent identity: annotation2481402
display id: annotation2481402
version: 1
top level: BBa_K2180006
location: https://synbiohub.org/public/igem/BBa_K2180006/annotation2481402/range2481402/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K2180006/annotation2481402/range2481402/1
type: Range
persistent identity: range2481402
display id: range2481402
version: 1
top level: BBa_K2180006
direction: direction/forward
start: 180
end: 187
orientation: inline

LacI Operator O1
type: SequenceAnnotation
persistent identity: annotation2481403
display id: annotation2481403
version: 1
top level: BBa_K2180006
location: https://synbiohub.org/public/igem/BBa_K2180006/annotation2481403/range2481403/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K2180006/annotation2481403/range2481403/1
type: Range
persistent identity: range2481403
display id: range2481403
version: 1
top level: BBa_K2180006
direction: direction/forward
start: 188
end: 208
orientation: inline

LacI Operator O1
type: SequenceAnnotation
persistent identity: annotation2481404
display id: annotation2481404
version: 1
top level: BBa_K2180006
location: https://synbiohub.org/public/igem/BBa_K2180006/annotation2481404/range2481404/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K2180006/annotation2481404/range2481404/1
type: Range
persistent identity: range2481404
display id: range2481404
version: 1
top level: BBa_K2180006
direction: direction/forward
start: 209
end: 229
orientation: inline

SacII restriction site
type: SequenceAnnotation
persistent identity: annotation2481405
display id: annotation2481405
version: 1
top level: BBa_K2180006
location: https://synbiohub.org/public/igem/BBa_K2180006/annotation2481405/range2481405/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K2180006/annotation2481405/range2481405/1
type: Range
persistent identity: range2481405
display id: range2481405
version: 1
top level: BBa_K2180006
direction: direction/forward
start: 230
end: 235
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2481406
display id: annotation2481406
version: 1
top level: BBa_K2180006
location: https://synbiohub.org/public/igem/BBa_K2180006/annotation2481406/range2481406/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K2180006/annotation2481406/range2481406/1
type: Range
persistent identity: range2481406
display id: range2481406
version: 1
top level: BBa_K2180006
direction: direction/forward
start: 236
end: 246
orientation: inline

NdeI
type: SequenceAnnotation
persistent identity: annotation2481418
display id: annotation2481418
version: 1
top level: BBa_K2180006
location: https://synbiohub.org/public/igem/BBa_K2180006/annotation2481418/range2481418/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K2180006/annotation2481418/range2481418/1
type: Range
persistent identity: range2481418
display id: range2481418
version: 1
top level: BBa_K2180006
direction: direction/forward
start: 254
end: 258
orientation: inline

https://synbiohub.org/public/igem/BBa_K2180012_sequence/1
type: Sequence
persistent identity: BBa K2180012 sequence
display id: BBa_K2180012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatcccaagcttggcaaaaatcagaccagacaaaagcggcaaatgaataagcggaacggggaaggatttgcggtcaagtccttcccttccgcacgtatcaattcgcaagcttttcctttataatagaatgaatgaatcgattgataatgataatcattatcaacatgtaaaggaggtgtctttatcccatggtactagatggtaaacgtgaaaccagtgacattgtacgatgttgctgagtatgcaggcgtcagctatcaaactgtaagtcgagtagttaaccaagccagccatgtgagcgcaaaaacgcgcgagaaggtggaagcggcgatggctgagttaaactatataccaaatagagtcgctcagcaattagccggcaaacagtccctgttgatcggtgtagcgacctcctctctggcattgcatgctccgtctcagatcgtggcagcaattaagtcacgtgctgatcaattaggagcttctgttgttgtcagcatggtggaaaggagcggagtcgaggcgtgcaaagcggcggttcataacctactagcccagcgcgtgtctgggcttattataaattaccccctggatgatcaagatgcaatcgcggttgaagcagcttgtacgaacgtcccagccttgttcctcgacgtcagtgaccagacgccgattaattccatcatctttagccacgaggacggaactcgcctcggcgtggagcatcttgtcgcgttgggacaccagcagattgctctactcgcggggccgttaagttctgtatcagcacgtttaagactcgcaggctggcataaatatttgaccagaaatcaaattcaaccaattgctgaacgcgaaggagattggtcggcaatgagcggcttccagcagacgatgcaaatgctgaatgaaggtattgttcctaccgcaatgttagtggccaacgaccaaatggcgctcggtgcgatgcgtgcaatcaccgaatcaggcttgcgtgtgggggctgatatctccgtggtcgggtatgacgatactgaagattcaagctgttatattccgcctttgacaactataaaacaagattttagactgctaggccagacatcagtagatcgactgctgcaactttcccaggggcaagcggttaaaggaaaccaactgttaccggtatctctcgtcaaacgcaagacaacattagccccgaatacccaaacagcatccccgcgcgctctcgctgactcacttatgcagcttgcccggcaggtcagtagactggaatcagggcaggcagctaatgacgaaaattatgcccttgtagcctaatgactttaataatgctgatgcaggtcatactagaggctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctaggtactagagccaagcttggtcgacgtcgaaaagtcaatgtatgaatggatacgggatatgaatcaataagtacgtgaaagagaaaagcaacccagatatgatagggaacttttctctttcttgttttacattgaatctttacaatcctattgatataatctaagctagtgtattttgcgtttaatagtttaattaaaattgtgagcggataacaattaattgtgagcggataacaattccgcggaaaggaggtgtctttatcatatgtactagagcatatgtccgtaattgctaaacaaatgacctacaaagtttacatgagcggcacagttaacggtcactatttcgaagtggagggagatggaaaaggaaaaccatacgaaggtgagcagaccgttaaactcaccgtaacgaagggtggaccacttccgtttgcctgggatattctaagtccgcaatgccaatacgggtcaattcctttcactaaataccctgaggacatcccggattacgttaagcaaagtttcccggaagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2180004_sequence/1
type: Sequence
persistent identity: BBa K2180004 sequence
display id: BBa_K2180004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatcccaagcttggcaaaaatcagaccagacaaaagcggcaaatgaataagcggaacggggaaggatttgcggtcaagtccttcccttccgcacgtatcaattcgcaagcttttcctttataatagaatgaatgaatcgattgataatgataatcattatcaacatgtaaaggaggtgtctttatcccatggtactagatggtaaacgtgaaaccagtgacattgtacgatgttgctgagtatgcaggcgtcagctatcaaactgtaagtcgagtagttaaccaagccagccatgtgagcgcaaaaacgcgcgagaaggtggaagcggcgatggctgagttaaactatataccaaatagagtcgctcagcaattagccggcaaacagtccctgttgatcggtgtagcgacctcctctctggcattgcatgctccgtctcagatcgtggcagcaattaagtcacgtgctgatcaattaggagcttctgttgttgtcagcatggtggaaaggagcggagtcgaggcgtgcaaagcggcggttcataacctactagcccagcgcgtgtctgggcttattataaattaccccctggatgatcaagatgcaatcgcggttgaagcagcttgtacgaacgtcccagccttgttcctcgacgtcagtgaccagacgccgattaattccatcatctttagccacgaggacggaactcgcctcggcgtggagcatcttgtcgcgttgggacaccagcagattgctctactcgcggggccgttaagttctgtatcagcacgtttaagactcgcaggctggcataaatatttgaccagaaatcaaattcaaccaattgctgaacgcgaaggagattggtcggcaatgagcggcttccagcagacgatgcaaatgctgaatgaaggtattgttcctaccgcaatgttagtggccaacgaccaaatggcgctcggtgcgatgcgtgcaatcaccgaatcaggcttgcgtgtgggggctgatatctccgtggtcgggtatgacgatactgaagattcaagctgttatattccgcctttgacaactataaaacaagattttagactgctaggccagacatcagtagatcgactgctgcaactttcccaggggcaagcggttaaaggaaaccaactgttaccggtatctctcgtcaaacgcaagacaacattagccccgaatacccaaacagcatccccgcgcgctctcgctgactcacttatgcagcttgcccggcaggtcagtagactggaatcagggcaggcagctaatgacgaaaattatgcccttgtagcctaatgactttaataatgctgatgcaggtcatactagaggctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2180018_sequence/1
type: Sequence
persistent identity: BBa K2180018 sequence
display id: BBa_K2180018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaagcttggtcgacgtcgaaaagtcaatgtatgaatggatacgggatatgaatcaataagtacgtgaaagagaaaagcaacccagatatgatagggaacttttctctttcttgttttacattgaatctttacaatcctattgatataatctaagctagtgtattttgcgtttaatagtttaattaaaattgtgagcggataacaattaattgtgagcggataacaattccgcggaaaggaggtgtctttatcatatgtactagagcatatgtccgtaattgctaaacaaatgacctacaaagtttacatgagcggcacagttaacggtcactatttcgaagtggagggagatggaaaaggaaaaccatacgaaggtgagcagaccgttaaactcaccgtaacgaagggtggaccacttccgtttgcctgggatattctaagtccgcaatgccaatacgggtcaattcctttcactaaataccctgaggacatcccggattacgttaagcaaagtttcccggaaggatacacatgggaacggattatgaactttgaagacggtgcggtctgcacagtctcgaatgatagtagcatccagggtaattgttttatttatcacgtcaagttctcaggccttaacttccctccgaacgggcccgtgatgcagaaaaagacgcaaggctgggagccaaacacggaacgcctcttcgcaagggatggaatgttgttaggcaataactttatggcgctcaaactggaaggcggtggacattatctgtgcgaatttaaaactacatataaagcaaaaaagccggtcaagatgccgggataccattatgtagatcgtaaactggacgtgaccaatcataataaggactatacatcagtggaacagtgcgaaatctcaatcgctcgtaagccagtcgtggccgctgcaaacgacgaaaactacgctttagtagcttaataacctaggtactagaggctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2180007_sequence/1
type: Sequence
persistent identity: BBa K2180007 sequence
display id: BBa_K2180007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatcccaagcttggcaaaaatcagaccagacaaaagcggcaaatgaataagcggaacggggaaggatttgcggtcaagtccttcccttccgcacgtatcaattcgcaagcttttcctttataatagaatgaatgaatcgattgataatgataatcattatcaacatgtaaaggaggtgtctttatcccatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2180008_sequence/1
type: Sequence
persistent identity: BBa K2180008 sequence
display id: BBa_K2180008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2180009_sequence/1
type: Sequence
persistent identity: BBa K2180009 sequence
display id: BBa_K2180009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtaaacgtgaaaccagtgacattgtacgatgttgctgagtatgcaggcgtcagctatcaaactgtaagtcgagtagttaaccaagccagccatgtgagcgcaaaaacgcgcgagaaggtggaagcggcgatggctgagttaaactatataccaaatagagtcgctcagcaattagccggcaaacagtccctgttgatcggtgtagcgacctcctctctggcattgcatgctccgtctcagatcgtggcagcaattaagtcacgtgctgatcaattaggagcttctgttgttgtcagcatggtggaaaggagcggagtcgaggcgtgcaaagcggcggttcataacctactagcccagcgcgtgtctgggcttattataaattaccccctggatgatcaagatgcaatcgcggttgaagcagcttgtacgaacgtcccagccttgttcctcgacgtcagtgaccagacgccgattaattccatcatctttagccacgaggacggaactcgcctcggcgtggagcatcttgtcgcgttgggacaccagcagattgctctactcgcggggccgttaagttctgtatcagcacgtttaagactcgcaggctggcataaatatttgaccagaaatcaaattcaaccaattgctgaacgcgaaggagattggtcggcaatgagcggcttccagcagacgatgcaaatgctgaatgaaggtattgttcctaccgcaatgttagtggccaacgaccaaatggcgctcggtgcgatgcgtgcaatcaccgaatcaggcttgcgtgtgggggctgatatctccgtggtcgggtatgacgatactgaagattcaagctgttatattccgcctttgacaactataaaacaagattttagactgctaggccagacatcagtagatcgactgctgcaactttcccaggggcaagcggttaaaggaaaccaactgttaccggtatctctcgtcaaacgcaagacaacattagccccgaatacccaaacagcatccccgcgcgctctcgctgactcacttatgcagcttgcccggcaggtcagtagactggaatcagggcaggcagctaatgacgaaaattatgcccttgtagcctaatgactttaataatgctgatgcaggtca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2180005_sequence/1
type: Sequence
persistent identity: BBa K2180005 sequence
display id: BBa_K2180005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catatgtccgtaattgctaaacaaatgacctacaaagtttacatgagcggcacagttaacggtcactatttcgaagtggagggagatggaaaaggaaaaccatacgaaggtgagcagaccgttaaactcaccgtaacgaagggtggaccacttccgtttgcctgggatattctaagtccgcaatgccaatacgggtcaattcctttcactaaataccctgaggacatcccggattacgttaagcaaagtttcccggaaggatacacatgggaacggattatgaactttgaagacggtgcggtctgcacagtctcgaatgatagtagcatccagggtaattgttttatttatcacgtcaagttctcaggccttaacttccctccgaacgggcccgtgatgcagaaaaagacgcaaggctgggagccaaacacggaacgcctcttcgcaagggatggaatgttgttaggcaataactttatggcgctcaaactggaaggcggtggacattatctgtgcgaatttaaaactacatataaagcaaaaaagccggtcaagatgccgggataccattatgtagatcgtaaactggacgtgaccaatcataataaggactatacatcagtggaacagtgcgaaatctcaatcgctcgtaagccagtcgtggccgctgcaaacgacgaaaactacgctttagtagcttaataacctagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2180006_sequence/1
type: Sequence
persistent identity: BBa K2180006 sequence
display id: BBa_K2180006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaagcttggtcgacgtcgaaaagtcaatgtatgaatggatacgggatatgaatcaataagtacgtgaaagagaaaagcaacccagatatgatagggaacttttctctttcttgttttacattgaatctttacaatcctattgatataatctaagctagtgtattttgcgtttaatagtttaattaaaattgtgagcggataacaattaattgtgagcggataacaattccgcggaaaggaggtgtctttatcatatg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z