LOCUS       BBa_K2180013            1145 bp    DNA     linear       05-Nov-2024
DEFINITION  Promoter + Zinc Operator - CzrA Repressor Binding Site + Blue 
            Pigment + Terminator
ACCESSION   BBa_K2180013
VERSION     BBa_K2180013.1  
FEATURES             Location/Qualifiers
     misc_feature    10..15
                     /label=SalI restriction site
     promoter        23..179
                     /label=PlepA
     misc_feature    180..187
                     /label=PacI restriction site
     misc_feature    188..208
                     /label=LacI Operator O1
     misc_feature    209..229
                     /label=LacI Operator O1
     misc_feature    230..235
                     /label=SacII restriction site
     RBS             236..246
                     /label=RBS
     misc_feature    254..258
                     /label=NdeI
     promoter        1..258
                     /label=BBa_K2180006
     misc_feature    273..303
                     /label=CzrA Repressor Binding Site
     misc_feature    267..272
                     /label=SacII Restriction Site
     misc_feature    304..311
                     /label=PacI Restriction Site
     promoter        267..311
                     /label=BBa_K2180001
     CDS             323..1024
                     /label=Pigment
     misc_feature    323..325
                     /label=start
     misc_feature    320..325
                     /label=Nde I restriction site
     misc_feature    1025..1030
                     /label=Avr II restriction site
     misc_feature    986..1018
                     /label=LVA tail
     CDS             320..1030
                     /label=BBa_K2180005
     misc_feature    1039..1044
                     /label=BmtI
     misc_feature    1045..1085
                     /label=Transcription terminator
     misc_feature    1094..1139
                     /label=Transcription terminator'
     misc_feature    1140..1145
                     /label=AvrII
     terminator      1039..1145
                     /label=BBa_K2180008
ORIGIN
        1 ccaagcttgg tcgacgtcga aaagtcaatg tatgaatgga tacgggatat gaatcaataa
       61 gtacgtgaaa gagaaaagca acccagatat gatagggaac ttttctcttt cttgttttac
      121 attgaatctt tacaatccta ttgatataat ctaagctagt gtattttgcg tttaatagtt
      181 taattaaaat tgtgagcgga taacaattaa ttgtgagcgg ataacaattc cgcggaaagg
      241 aggtgtcttt atcatatgta ctagagccgc ggcttatata tgagtatatg ctcatatata
      301 taattaatta atactagagc atatgtccgt aattgctaaa caaatgacct acaaagttta
      361 catgagcggc acagttaacg gtcactattt cgaagtggag ggagatggaa aaggaaaacc
      421 atacgaaggt gagcagaccg ttaaactcac cgtaacgaag ggtggaccac ttccgtttgc
      481 ctgggatatt ctaagtccgc aatgccaata cgggtcaatt cctttcacta aataccctga
      541 ggacatcccg gattacgtta agcaaagttt cccggaagga tacacatggg aacggattat
      601 gaactttgaa gacggtgcgg tctgcacagt ctcgaatgat agtagcatcc agggtaattg
      661 ttttatttat cacgtcaagt tctcaggcct taacttccct ccgaacgggc ccgtgatgca
      721 gaaaaagacg caaggctggg agccaaacac ggaacgcctc ttcgcaaggg atggaatgtt
      781 gttaggcaat aactttatgg cgctcaaact ggaaggcggt ggacattatc tgtgcgaatt
      841 taaaactaca tataaagcaa aaaagccggt caagatgccg ggataccatt atgtagatcg
      901 taaactggac gtgaccaatc ataataagga ctatacatca gtggaacagt gcgaaatctc
      961 aatcgctcgt aagccagtcg tggccgctgc aaacgacgaa aactacgctt tagtagctta
     1021 ataacctagg tactagaggc tagctcacac tggctcacct tcgggtgggc ctttctgcgt
     1081 ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt ttattatttc
     1141 ctagg
//