LOCUS       BBa_K2180014            1154 bp    DNA     linear       05-Nov-2024
DEFINITION  Promoter + Fructose Operator - FruR Binding Site + Blue Pigment + 
            Terminator
ACCESSION   BBa_K2180014
VERSION     BBa_K2180014.1  
FEATURES             Location/Qualifiers
     misc_feature    10..15
                     /label=SalI restriction site
     promoter        23..179
                     /label=PlepA
     misc_feature    180..187
                     /label=PacI restriction site
     misc_feature    188..208
                     /label=LacI Operator O1
     misc_feature    209..229
                     /label=LacI Operator O1
     misc_feature    230..235
                     /label=SacII restriction site
     RBS             236..246
                     /label=RBS
     misc_feature    254..258
                     /label=NdeI
     promoter        1..258
                     /label=BBa_K2180006
     misc_feature    267..272
                     /label=SacII Restriction Site
     misc_feature    273..293
                     /label=FruR Binding Site 1
     misc_feature    294..312
                     /label=FruR Binding Site 2
     misc_feature    313..320
                     /label=PacI Restriction Site
     promoter        267..320
                     /label=BBa_K2180002
     CDS             332..1033
                     /label=Pigment
     misc_feature    332..334
                     /label=start
     misc_feature    329..334
                     /label=Nde I restriction site
     misc_feature    1034..1039
                     /label=Avr II restriction site
     misc_feature    995..1027
                     /label=LVA tail
     CDS             329..1039
                     /label=BBa_K2180005
     misc_feature    1048..1053
                     /label=BmtI
     misc_feature    1054..1094
                     /label=Transcription terminator
     misc_feature    1103..1148
                     /label=Transcription terminator'
     misc_feature    1149..1154
                     /label=AvrII
     terminator      1048..1154
                     /label=BBa_K2180008
ORIGIN
        1 ccaagcttgg tcgacgtcga aaagtcaatg tatgaatgga tacgggatat gaatcaataa
       61 gtacgtgaaa gagaaaagca acccagatat gatagggaac ttttctcttt cttgttttac
      121 attgaatctt tacaatccta ttgatataat ctaagctagt gtattttgcg tttaatagtt
      181 taattaaaat tgtgagcgga taacaattaa ttgtgagcgg ataacaattc cgcggaaagg
      241 aggtgtcttt atcatatgta ctagagccgc ggtgaaagat tttgatagtt attgaatgaa
      301 tttgaatgat ttttaattaa tactagagca tatgtccgta attgctaaac aaatgaccta
      361 caaagtttac atgagcggca cagttaacgg tcactatttc gaagtggagg gagatggaaa
      421 aggaaaacca tacgaaggtg agcagaccgt taaactcacc gtaacgaagg gtggaccact
      481 tccgtttgcc tgggatattc taagtccgca atgccaatac gggtcaattc ctttcactaa
      541 ataccctgag gacatcccgg attacgttaa gcaaagtttc ccggaaggat acacatggga
      601 acggattatg aactttgaag acggtgcggt ctgcacagtc tcgaatgata gtagcatcca
      661 gggtaattgt tttatttatc acgtcaagtt ctcaggcctt aacttccctc cgaacgggcc
      721 cgtgatgcag aaaaagacgc aaggctggga gccaaacacg gaacgcctct tcgcaaggga
      781 tggaatgttg ttaggcaata actttatggc gctcaaactg gaaggcggtg gacattatct
      841 gtgcgaattt aaaactacat ataaagcaaa aaagccggtc aagatgccgg gataccatta
      901 tgtagatcgt aaactggacg tgaccaatca taataaggac tatacatcag tggaacagtg
      961 cgaaatctca atcgctcgta agccagtcgt ggccgctgca aacgacgaaa actacgcttt
     1021 agtagcttaa taacctaggt actagaggct agctcacact ggctcacctt cgggtgggcc
     1081 tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat ccggcttttt
     1141 tattatttcc tagg
//