LOCUS       BBa_K2180015            1132 bp    DNA     linear       05-Nov-2024
DEFINITION  Promoter + Sucrose Operator - DegU Binding Site + Blue Pigment + 
            Terminator
ACCESSION   BBa_K2180015
VERSION     BBa_K2180015.1  
FEATURES             Location/Qualifiers
     misc_feature    10..15
                     /label=SalI restriction site
     promoter        23..179
                     /label=PlepA
     misc_feature    180..187
                     /label=PacI restriction site
     misc_feature    188..208
                     /label=LacI Operator O1
     misc_feature    209..229
                     /label=LacI Operator O1
     misc_feature    230..235
                     /label=SacII restriction site
     RBS             236..246
                     /label=RBS
     misc_feature    254..258
                     /label=NdeI
     promoter        1..258
                     /label=BBa_K2180006
     misc_feature    267..272
                     /label=SacII Restriction Site
     misc_feature    273..280
                     /label=DegU Binding Site 1
     misc_feature    283..290
                     /label=DegU Binding SIte 2
     misc_feature    291..298
                     /label=PacI Restriction Site
     promoter        267..298
                     /label=BBa_K2180003
     CDS             310..1011
                     /label=Pigment
     misc_feature    310..312
                     /label=start
     misc_feature    307..312
                     /label=Nde I restriction site
     misc_feature    1012..1017
                     /label=Avr II restriction site
     misc_feature    973..1005
                     /label=LVA tail
     CDS             307..1017
                     /label=BBa_K2180005
     misc_feature    1026..1031
                     /label=BmtI
     misc_feature    1032..1072
                     /label=Transcription terminator
     misc_feature    1081..1126
                     /label=Transcription terminator'
     misc_feature    1127..1132
                     /label=AvrII
     terminator      1026..1132
                     /label=BBa_K2180008
ORIGIN
        1 ccaagcttgg tcgacgtcga aaagtcaatg tatgaatgga tacgggatat gaatcaataa
       61 gtacgtgaaa gagaaaagca acccagatat gatagggaac ttttctcttt cttgttttac
      121 attgaatctt tacaatccta ttgatataat ctaagctagt gtattttgcg tttaatagtt
      181 taattaaaat tgtgagcgga taacaattaa ttgtgagcgg ataacaattc cgcggaaagg
      241 aggtgtcttt atcatatgta ctagagccgc gggacatttt ggttttacag ttaattaata
      301 ctagagcata tgtccgtaat tgctaaacaa atgacctaca aagtttacat gagcggcaca
      361 gttaacggtc actatttcga agtggaggga gatggaaaag gaaaaccata cgaaggtgag
      421 cagaccgtta aactcaccgt aacgaagggt ggaccacttc cgtttgcctg ggatattcta
      481 agtccgcaat gccaatacgg gtcaattcct ttcactaaat accctgagga catcccggat
      541 tacgttaagc aaagtttccc ggaaggatac acatgggaac ggattatgaa ctttgaagac
      601 ggtgcggtct gcacagtctc gaatgatagt agcatccagg gtaattgttt tatttatcac
      661 gtcaagttct caggccttaa cttccctccg aacgggcccg tgatgcagaa aaagacgcaa
      721 ggctgggagc caaacacgga acgcctcttc gcaagggatg gaatgttgtt aggcaataac
      781 tttatggcgc tcaaactgga aggcggtgga cattatctgt gcgaatttaa aactacatat
      841 aaagcaaaaa agccggtcaa gatgccggga taccattatg tagatcgtaa actggacgtg
      901 accaatcata ataaggacta tacatcagtg gaacagtgcg aaatctcaat cgctcgtaag
      961 ccagtcgtgg ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acctaggtac
     1021 tagaggctag ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     1081 agagaatata aaaagccaga ttattaatcc ggctttttta ttatttccta gg
//