LOCUS       BBa_K2180018            1092 bp    DNA     linear       26-Dec-2024
DEFINITION  Promoter PlepA + Operator LacI + Blue Pigment + Double terminator
ACCESSION   BBa_K2180018
VERSION     BBa_K2180018.1  
FEATURES             Location/Qualifiers
     misc_feature    10..15
                     /label=SalI restriction site
     promoter        23..179
                     /label=PlepA
     misc_feature    180..187
                     /label=PacI restriction site
     misc_feature    188..208
                     /label=LacI Operator O1
     misc_feature    209..229
                     /label=LacI Operator O1
     misc_feature    230..235
                     /label=SacII restriction site
     RBS             236..246
                     /label=RBS
     misc_feature    254..258
                     /label=NdeI
     promoter        1..258
                     /label=BBa_K2180006
     CDS             270..971
                     /label=Pigment
     misc_feature    270..272
                     /label=start
     misc_feature    267..272
                     /label=Nde I restriction site
     misc_feature    972..977
                     /label=Avr II restriction site
     misc_feature    933..965
                     /label=LVA tail
     CDS             267..977
                     /label=BBa_K2180005
     misc_feature    986..991
                     /label=BmtI
     misc_feature    992..1032
                     /label=Transcription terminator
     misc_feature    1041..1086
                     /label=Transcription terminator'
     misc_feature    1087..1092
                     /label=AvrII
     terminator      986..1092
                     /label=BBa_K2180008
ORIGIN
        1 ccaagcttgg tcgacgtcga aaagtcaatg tatgaatgga tacgggatat gaatcaataa
       61 gtacgtgaaa gagaaaagca acccagatat gatagggaac ttttctcttt cttgttttac
      121 attgaatctt tacaatccta ttgatataat ctaagctagt gtattttgcg tttaatagtt
      181 taattaaaat tgtgagcgga taacaattaa ttgtgagcgg ataacaattc cgcggaaagg
      241 aggtgtcttt atcatatgta ctagagcata tgtccgtaat tgctaaacaa atgacctaca
      301 aagtttacat gagcggcaca gttaacggtc actatttcga agtggaggga gatggaaaag
      361 gaaaaccata cgaaggtgag cagaccgtta aactcaccgt aacgaagggt ggaccacttc
      421 cgtttgcctg ggatattcta agtccgcaat gccaatacgg gtcaattcct ttcactaaat
      481 accctgagga catcccggat tacgttaagc aaagtttccc ggaaggatac acatgggaac
      541 ggattatgaa ctttgaagac ggtgcggtct gcacagtctc gaatgatagt agcatccagg
      601 gtaattgttt tatttatcac gtcaagttct caggccttaa cttccctccg aacgggcccg
      661 tgatgcagaa aaagacgcaa ggctgggagc caaacacgga acgcctcttc gcaagggatg
      721 gaatgttgtt aggcaataac tttatggcgc tcaaactgga aggcggtgga cattatctgt
      781 gcgaatttaa aactacatat aaagcaaaaa agccggtcaa gatgccggga taccattatg
      841 tagatcgtaa actggacgtg accaatcata ataaggacta tacatcagtg gaacagtgcg
      901 aaatctcaat cgctcgtaag ccagtcgtgg ccgctgcaaa cgacgaaaac tacgctttag
      961 tagcttaata acctaggtac tagaggctag ctcacactgg ctcaccttcg ggtgggcctt
     1021 tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta
     1081 ttatttccta gg
//