BBa_K2180008 1 BBa_K2180008 Terminator 2016-08-04T11:00:00Z 2016-10-13T10:53:37Z This is a double terminator we use in our project for the pigment production. He is made of a transcriptional terminator in forward and reverse direction. There is a BmtI restriction site before the first terminator (forward) and an AvrII restriction site after the second terminator (reverse). false false _2651_ 25719 25719 9 false false Sofiane Safi-Stibler annotation2481414 1 Transcription terminator range2481414 1 7 47 annotation2481415 1 Transcription terminator' range2481415 1 56 101 annotation2481416 1 AvrII range2481416 1 102 107 annotation2481413 1 BmtI range2481413 1 1 6 BBa_K2180006 1 BBa_K2180006 PLacO - Promoter LacO for B. subtilis 2016-08-04T11:00:00Z 2016-10-13T10:58:17Z The part is made of a previous IGEM part (add part) This part is made of PlepA promoter, two LacI binding sites and a RBS. There is a PacI restriction site before LacI binding sites and a SacII restriction site after them. They allows us to remplace the LacI binding sites by another operating sequence. A SalI restriction sites is presents before the PlepA sequence that may be used to insert the whole sequence. We use this part in our project to promote the pigment expression under the control of LacI. false false _2651_ 25719 25719 9 false false Sofiane Safi-Stibler annotation2481402 1 PacI restriction site range2481402 1 180 187 annotation2481405 1 SacII restriction site range2481405 1 230 235 annotation2481418 1 NdeI range2481418 1 254 258 annotation2481400 1 SalI restriction site range2481400 1 10 15 annotation2481406 1 RBS range2481406 1 236 246 annotation2481404 1 LacI Operator O1 range2481404 1 209 229 annotation2481401 1 PlepA range2481401 1 23 179 annotation2481403 1 LacI Operator O1 range2481403 1 188 208 BBa_K2180005 1 BBa_K2180005 Pigment Blue for B. subtilis with a LVA tail and restriction site (Blue Pigment) 2016-08-04T11:00:00Z 2016-10-21T08:21:50Z This part come from a previous IGEM part (add part) This sequence is coding for a blue pigment we use in our detection system as a reporter. false false _2651_ 32196 25719 9 false We had to improve the codon usage for B. subtilis false Sofiane Safi-Stibler annotation2527142 1 Avr II restriction site range2527142 1 706 711 annotation2527143 1 LVA tail range2527143 1 667 699 annotation2527137 1 start range2527137 1 4 6 annotation2481399 1 Pigment range2481399 1 4 705 annotation2527141 1 Nde I restriction site range2527141 1 1 6 BBa_K2180018 1 BBa_K2180018 Promoter PlepA + Operator LacI + Blue Pigment + Double terminator 2016-10-13T11:00:00Z 2016-10-29T06:47:03Z Lac I Pig Lac I Pig false false _2651_ 32196 32196 9 false Lac I Pig false Wilhelm Vaysse-Zinkh??fer component2531013 1 BBa_K2180005 component2531007 1 BBa_K2180006 component2531018 1 BBa_K2180008 annotation2531007 1 BBa_K2180006 range2531007 1 1 258 annotation2531018 1 BBa_K2180008 range2531018 1 986 1092 annotation2531013 1 BBa_K2180005 range2531013 1 267 977 BBa_K2180006_sequence 1 ccaagcttggtcgacgtcgaaaagtcaatgtatgaatggatacgggatatgaatcaataagtacgtgaaagagaaaagcaacccagatatgatagggaacttttctctttcttgttttacattgaatctttacaatcctattgatataatctaagctagtgtattttgcgtttaatagtttaattaaaattgtgagcggataacaattaattgtgagcggataacaattccgcggaaaggaggtgtctttatcatatg BBa_K2180018_sequence 1 ccaagcttggtcgacgtcgaaaagtcaatgtatgaatggatacgggatatgaatcaataagtacgtgaaagagaaaagcaacccagatatgatagggaacttttctctttcttgttttacattgaatctttacaatcctattgatataatctaagctagtgtattttgcgtttaatagtttaattaaaattgtgagcggataacaattaattgtgagcggataacaattccgcggaaaggaggtgtctttatcatatgtactagagcatatgtccgtaattgctaaacaaatgacctacaaagtttacatgagcggcacagttaacggtcactatttcgaagtggagggagatggaaaaggaaaaccatacgaaggtgagcagaccgttaaactcaccgtaacgaagggtggaccacttccgtttgcctgggatattctaagtccgcaatgccaatacgggtcaattcctttcactaaataccctgaggacatcccggattacgttaagcaaagtttcccggaaggatacacatgggaacggattatgaactttgaagacggtgcggtctgcacagtctcgaatgatagtagcatccagggtaattgttttatttatcacgtcaagttctcaggccttaacttccctccgaacgggcccgtgatgcagaaaaagacgcaaggctgggagccaaacacggaacgcctcttcgcaagggatggaatgttgttaggcaataactttatggcgctcaaactggaaggcggtggacattatctgtgcgaatttaaaactacatataaagcaaaaaagccggtcaagatgccgggataccattatgtagatcgtaaactggacgtgaccaatcataataaggactatacatcagtggaacagtgcgaaatctcaatcgctcgtaagccagtcgtggccgctgcaaacgacgaaaactacgctttagtagcttaataacctaggtactagaggctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagg BBa_K2180005_sequence 1 catatgtccgtaattgctaaacaaatgacctacaaagtttacatgagcggcacagttaacggtcactatttcgaagtggagggagatggaaaaggaaaaccatacgaaggtgagcagaccgttaaactcaccgtaacgaagggtggaccacttccgtttgcctgggatattctaagtccgcaatgccaatacgggtcaattcctttcactaaataccctgaggacatcccggattacgttaagcaaagtttcccggaaggatacacatgggaacggattatgaactttgaagacggtgcggtctgcacagtctcgaatgatagtagcatccagggtaattgttttatttatcacgtcaagttctcaggccttaacttccctccgaacgggcccgtgatgcagaaaaagacgcaaggctgggagccaaacacggaacgcctcttcgcaagggatggaatgttgttaggcaataactttatggcgctcaaactggaaggcggtggacattatctgtgcgaatttaaaactacatataaagcaaaaaagccggtcaagatgccgggataccattatgtagatcgtaaactggacgtgaccaatcataataaggactatacatcagtggaacagtgcgaaatctcaatcgctcgtaagccagtcgtggccgctgcaaacgacgaaaactacgctttagtagcttaataacctagg BBa_K2180008_sequence 1 gctagctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcctagg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z