LOCUS       BBa_K218002             2144 bp    DNA     linear       26-Dec-2024
DEFINITION  LuxOU under constitutive expression of TetR repressible promoter
ACCESSION   BBa_K218002
VERSION     BBa_K218002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             67..74
                     /label=RBS
     misc_feature    93..95
                     /label=Start of LuxO
     misc_feature    1452..1454
                     /label=Stop for LuxO
     CDS             93..1454
                     /label=LuxO
     misc_feature    1451..1453
                     /label=Start of LuxU
     misc_feature    1793..1795
                     /label=Stop for LuxU
     CDS             1451..1795
                     /label=LuxU
     misc_feature    63..2007
                     /label=BBa_K218001
     stem_loop       2027..2070
                     /label=stem_loop
     misc_feature    2016..2095
                     /label=BBa_B0010
     terminator      2016..2095
                     /label=BBa_B0010
     stem_loop       2111..2130
                     /label=T7 TE
     misc_feature    2137..2137
                     /label=stop
     polyA_site      2131..2144
                     /label=polya
     misc_feature    2104..2144
                     /label=BBa_B0012
     terminator      2104..2144
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agacagaagg tcaaaagtct cgttatctac ttatggtaga agacaccgca tccgttgcgg
      121 cactttaccg ctcttacctc acgccacttg gcatcgatat caatattgtt ggaacaggca
      181 gagacgccat tgaaagcctg aaccatcgca ttcctgatct tattctgctc gatcttcgtc
      241 tacctgatat gacggggatg gacgtattgc acgcggtgaa gaaaagccac ccagacgtgc
      301 caatcatctt catgacagcc catggttcta tcgatactgc ggtagaggcg atgcgccacg
      361 gttctcaaga cttcctaatc aaaccatgtg aagcagaccg tttacgtgtc acggtgaaca
      421 atgcgatccg taaagcaacc aaattaaaga atgaagctga caaccccggt aaccaaaatt
      481 accaaggctt catcggcagt agccaaacga tgcagcaggt ttaccgcacc attgactcgg
      541 cagcgagcag taaagcgagt attttcatca cgggtgaaag tggtacgggt aaagaagtgt
      601 gtgccgaagc gattcacgca gcaagcaaac gcggtgataa gccgtttatc gccatcaact
      661 gtgcggcaat cccgaaagac cttattgaaa gtgagctgtt tggtcacgta aaaggtgcgt
      721 ttactggtgc tgcgaatgac cgacaaggtg cggcagagct tgctgatggc ggcaccttgt
      781 tccttgatga actctgtgaa atggacttgg atcttcaaac taagctattg cgctttatcc
      841 aaacgggtac attccaaaaa gtcggttctt ctaaaatgaa gagcgtggat gtgcgctttg
      901 tgtgtgcaac taaccgagac ccttggaaag aagtgcaaga aggccgtttc cgtgaagact
      961 tgtattaccg tttgtacgtg attcctttgc accttccgcc gctgcgtgag cgtggtaaag
     1021 acgttattga aattgcatac tcgttgcttg gttatatgtc tcatgaggaa ggtaagagtt
     1081 tcgtccgttt cgcacaagac gtgattgaaa gattcaacag ctacgaatgg ccgggtaacg
     1141 ttcgccagtt gcaaaacgta ttgcgtaata tcgtggtact gaacaatggc aaagagatca
     1201 cgctggatat gttaccgcca ccactgaatc agcctgttgt gcgccaatcg gtagcaaaat
     1261 tcattgaacc tgacattatg acggtgtcag atattatgcc gctttggatg acagagaaaa
     1321 tggctattga gcaggcaatt caagcgtgtg aaggcaacat tccacgcgct gctggctatt
     1381 tggatgttag tccatcaacg atttatcgca agttgcaagc ttggaatagc aaggacgaaa
     1441 aacaaaacgt atgaatacgg acgtattaaa tcagcaaaaa attgaagaac tgtctgcgga
     1501 aattggtagc gataatgttc ctgttttgct tgatattttt cttggggaaa tggactccta
     1561 cattggcact ttaactgaac ttcagggctc agagcagctg ttgtatttaa aagagatcag
     1621 ccacgcactg aaaagtagtg ctgccagctt tggcgcagat cgattgtgtg aacgagcgat
     1681 tgccatcgac aagaaagcaa aagcgaatca attgcaagag caggggatgg agacgagcga
     1741 aatgctcgct ttacttcata tcactcgtga cgcctaccgt tcttggacaa actaacgttt
     1801 cgagcaagac attaagcgca acgtaaaaac acaaagccct tccggtgtgg aagggctttt
     1861 ttgtttgggg agtttgctcc gaatcgaagc cgctttctca atgcttttcg tctagttaga
     1921 cagtaagcgc tccataaaac ccgcattcta atcgcctagc gcgaagaata agatcaagtc
     1981 tccaaccatg aggagatttg aaatgggtac tagagccagg catcaaataa aacgaaaggc
     2041 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     2101 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//