LOCUS       BBa_K218005             2281 bp    DNA     linear       26-Nov-2024
DEFINITION  LuxOU generator with terminator upstream
ACCESSION   BBa_K218005
VERSION     BBa_K218005.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    138..156
                     /label=TetR 1
     misc_feature    138..191
                     /label=BBa_R0040
     promoter        157..162
                     /label=-35
     misc_feature    163..181
                     /label=TetR 2
     promoter        180..185
                     /label=-10
     promoter        138..191
                     /label=BBa_R0040
     RBS             204..211
                     /label=RBS
     misc_feature    230..232
                     /label=Start of LuxO
     misc_feature    1589..1591
                     /label=Stop for LuxO
     CDS             230..1591
                     /label=LuxO
     misc_feature    1588..1590
                     /label=Start of LuxU
     misc_feature    1930..1932
                     /label=Stop for LuxU
     CDS             1588..1932
                     /label=LuxU
     misc_feature    200..2144
                     /label=BBa_K218001
     stem_loop       2164..2207
                     /label=stem_loop
     misc_feature    2153..2232
                     /label=BBa_B0010
     terminator      2153..2232
                     /label=BBa_B0010
     stem_loop       2248..2267
                     /label=T7 TE
     misc_feature    2274..2274
                     /label=stop
     polyA_site      2268..2281
                     /label=polya
     misc_feature    2241..2281
                     /label=BBa_B0012
     terminator      2241..2281
                     /label=BBa_B0012
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag
      181 atactgagca ctactagaga cagaaggtca aaagtctcgt tatctactta tggtagaaga
      241 caccgcatcc gttgcggcac tttaccgctc ttacctcacg ccacttggca tcgatatcaa
      301 tattgttgga acaggcagag acgccattga aagcctgaac catcgcattc ctgatcttat
      361 tctgctcgat cttcgtctac ctgatatgac ggggatggac gtattgcacg cggtgaagaa
      421 aagccaccca gacgtgccaa tcatcttcat gacagcccat ggttctatcg atactgcggt
      481 agaggcgatg cgccacggtt ctcaagactt cctaatcaaa ccatgtgaag cagaccgttt
      541 acgtgtcacg gtgaacaatg cgatccgtaa agcaaccaaa ttaaagaatg aagctgacaa
      601 ccccggtaac caaaattacc aaggcttcat cggcagtagc caaacgatgc agcaggttta
      661 ccgcaccatt gactcggcag cgagcagtaa agcgagtatt ttcatcacgg gtgaaagtgg
      721 tacgggtaaa gaagtgtgtg ccgaagcgat tcacgcagca agcaaacgcg gtgataagcc
      781 gtttatcgcc atcaactgtg cggcaatccc gaaagacctt attgaaagtg agctgtttgg
      841 tcacgtaaaa ggtgcgttta ctggtgctgc gaatgaccga caaggtgcgg cagagcttgc
      901 tgatggcggc accttgttcc ttgatgaact ctgtgaaatg gacttggatc ttcaaactaa
      961 gctattgcgc tttatccaaa cgggtacatt ccaaaaagtc ggttcttcta aaatgaagag
     1021 cgtggatgtg cgctttgtgt gtgcaactaa ccgagaccct tggaaagaag tgcaagaagg
     1081 ccgtttccgt gaagacttgt attaccgttt gtacgtgatt cctttgcacc ttccgccgct
     1141 gcgtgagcgt ggtaaagacg ttattgaaat tgcatactcg ttgcttggtt atatgtctca
     1201 tgaggaaggt aagagtttcg tccgtttcgc acaagacgtg attgaaagat tcaacagcta
     1261 cgaatggccg ggtaacgttc gccagttgca aaacgtattg cgtaatatcg tggtactgaa
     1321 caatggcaaa gagatcacgc tggatatgtt accgccacca ctgaatcagc ctgttgtgcg
     1381 ccaatcggta gcaaaattca ttgaacctga cattatgacg gtgtcagata ttatgccgct
     1441 ttggatgaca gagaaaatgg ctattgagca ggcaattcaa gcgtgtgaag gcaacattcc
     1501 acgcgctgct ggctatttgg atgttagtcc atcaacgatt tatcgcaagt tgcaagcttg
     1561 gaatagcaag gacgaaaaac aaaacgtatg aatacggacg tattaaatca gcaaaaaatt
     1621 gaagaactgt ctgcggaaat tggtagcgat aatgttcctg ttttgcttga tatttttctt
     1681 ggggaaatgg actcctacat tggcacttta actgaacttc agggctcaga gcagctgttg
     1741 tatttaaaag agatcagcca cgcactgaaa agtagtgctg ccagctttgg cgcagatcga
     1801 ttgtgtgaac gagcgattgc catcgacaag aaagcaaaag cgaatcaatt gcaagagcag
     1861 gggatggaga cgagcgaaat gctcgcttta cttcatatca ctcgtgacgc ctaccgttct
     1921 tggacaaact aacgtttcga gcaagacatt aagcgcaacg taaaaacaca aagcccttcc
     1981 ggtgtggaag ggcttttttg tttggggagt ttgctccgaa tcgaagccgc tttctcaatg
     2041 cttttcgtct agttagacag taagcgctcc ataaaacccg cattctaatc gcctagcgcg
     2101 aagaataaga tcaagtctcc aaccatgagg agatttgaaa tgggtactag agccaggcat
     2161 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2221 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2281 a
//