##gff-version 3
##sequence-region BBa_K218005 1 2281
BBa_K218005	.	terminator	2153	2232	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K218005	.	stem_loop	2164	2207	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K218005	.	engineered_region	2153	2232	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K218005	.	terminator	2241	2281	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K218005	.	stem_loop	2248	2267	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K218005	.	polyA_site	2268	2281	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K218005	.	stop_codon	2274	2274	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K218005	.	engineered_region	2241	2281	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K218005	.	promoter	138	191	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K218005	.	promoter	157	162	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K218005	.	engineered_region	138	191	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K218005	.	non_covalent_binding_site	138	156	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K218005	.	non_covalent_binding_site	163	181	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K218005	.	promoter	180	185	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K218005	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K218005	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K218005	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K218005	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K218005	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K218005	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K218005	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K218005	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K218005	.	engineered_region	200	2144	.	+	0	ID=BBa_K218001;Name=BBa_K218001
BBa_K218005	.	CDS	1588	1932	.	+	0	ID=annotation2048776;Name=LuxU;Parent=BBa_K218001
BBa_K218005	.	CDS	230	1591	.	+	0	ID=annotation2048773;Name=LuxO;Parent=BBa_K218001
BBa_K218005	.	ribosome_entry_site	204	211	.	+	0	ID=annotation2048769;Name=RBS;Parent=BBa_K218001
BBa_K218005	.	start_codon	230	232	.	+	0	ID=annotation2048770;Name=Start of LuxO;Parent=BBa_K218001
BBa_K218005	.	stop_codon	1930	1932	.	+	0	ID=annotation2048775;Name=Stop for LuxU;Parent=BBa_K218001
BBa_K218005	.	start_codon	1588	1590	.	+	0	ID=annotation2048774;Name=Start of LuxU;Parent=BBa_K218001
BBa_K218005	.	stop_codon	1589	1591	.	+	0	ID=annotation2048771;Name=Stop for LuxO;Parent=BBa_K218001
>BBa_K218005
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagatt
gacatccctatcagtgatagagatactgagcactactagagacagaaggtcaaaagtctcgttatctacttatggtaga
agacaccgcatccgttgcggcactttaccgctcttacctcacgccacttggcatcgatatcaatattgttggaacaggc
agagacgccattgaaagcctgaaccatcgcattcctgatcttattctgctcgatcttcgtctacctgatatgacgggga
tggacgtattgcacgcggtgaagaaaagccacccagacgtgccaatcatcttcatgacagcccatggttctatcgatac
tgcggtagaggcgatgcgccacggttctcaagacttcctaatcaaaccatgtgaagcagaccgtttacgtgtcacggtg
aacaatgcgatccgtaaagcaaccaaattaaagaatgaagctgacaaccccggtaaccaaaattaccaaggcttcatcg
gcagtagccaaacgatgcagcaggtttaccgcaccattgactcggcagcgagcagtaaagcgagtattttcatcacggg
tgaaagtggtacgggtaaagaagtgtgtgccgaagcgattcacgcagcaagcaaacgcggtgataagccgtttatcgcc
atcaactgtgcggcaatcccgaaagaccttattgaaagtgagctgtttggtcacgtaaaaggtgcgtttactggtgctg
cgaatgaccgacaaggtgcggcagagcttgctgatggcggcaccttgttccttgatgaactctgtgaaatggacttgga
tcttcaaactaagctattgcgctttatccaaacgggtacattccaaaaagtcggttcttctaaaatgaagagcgtggat
gtgcgctttgtgtgtgcaactaaccgagacccttggaaagaagtgcaagaaggccgtttccgtgaagacttgtattacc
gtttgtacgtgattcctttgcaccttccgccgctgcgtgagcgtggtaaagacgttattgaaattgcatactcgttgct
tggttatatgtctcatgaggaaggtaagagtttcgtccgtttcgcacaagacgtgattgaaagattcaacagctacgaa
tggccgggtaacgttcgccagttgcaaaacgtattgcgtaatatcgtggtactgaacaatggcaaagagatcacgctgg
atatgttaccgccaccactgaatcagcctgttgtgcgccaatcggtagcaaaattcattgaacctgacattatgacggt
gtcagatattatgccgctttggatgacagagaaaatggctattgagcaggcaattcaagcgtgtgaaggcaacattcca
cgcgctgctggctatttggatgttagtccatcaacgatttatcgcaagttgcaagcttggaatagcaaggacgaaaaac
aaaacgtatgaatacggacgtattaaatcagcaaaaaattgaagaactgtctgcggaaattggtagcgataatgttcct
gttttgcttgatatttttcttggggaaatggactcctacattggcactttaactgaacttcagggctcagagcagctgt
tgtatttaaaagagatcagccacgcactgaaaagtagtgctgccagctttggcgcagatcgattgtgtgaacgagcgat
tgccatcgacaagaaagcaaaagcgaatcaattgcaagagcaggggatggagacgagcgaaatgctcgctttacttcat
atcactcgtgacgcctaccgttcttggacaaactaacgtttcgagcaagacattaagcgcaacgtaaaaacacaaagcc
cttccggtgtggaagggcttttttgtttggggagtttgctccgaatcgaagccgctttctcaatgcttttcgtctagtt
agacagtaagcgctccataaaacccgcattctaatcgcctagcgcgaagaataagatcaagtctccaaccatgaggaga
tttgaaatgggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata