LOCUS BBa_K218006 6114 bp DNA linear 27-Dec-2024 DEFINITION LuxPQ followed by terminator and LuxOU generator ACCESSION BBa_K218006 VERSION BBa_K218006.1 FEATURES Location/Qualifiers misc_feature 30..32 /label=start of LuxP misc_feature 1127..1129 /label=start of LuxQ misc_feature 1125..1127 /label=stop for LuxP CDS 30..1127 /label=LuxP misc_feature 3704..3706 /label=stop for LuxQ CDS 1127..3706 /label=LuxQ misc_feature 599..599 /label=Silent mutation to remove EcoRI site misc_feature 1465..1465 /label=Silent mutation to remove EcoRI site misc_feature 1618..1618 /label=Silent mutation to remove XbaI site misc_feature 1..3825 /label=BBa_K218000 stem_loop 3845..3888 /label=stem_loop misc_feature 3834..3913 /label=BBa_B0010 terminator 3834..3913 /label=BBa_B0010 stem_loop 3929..3948 /label=T7 TE misc_feature 3955..3955 /label=stop polyA_site 3949..3962 /label=polya misc_feature 3922..3962 /label=BBa_B0012 terminator 3922..3962 /label=BBa_B0012 misc_feature 3971..3989 /label=TetR 1 misc_feature 3971..4024 /label=BBa_R0040 promoter 3990..3995 /label=-35 misc_feature 3996..4014 /label=TetR 2 promoter 4013..4018 /label=-10 promoter 3971..4024 /label=BBa_R0040 RBS 4037..4044 /label=RBS misc_feature 4063..4065 /label=Start of LuxO misc_feature 5422..5424 /label=Stop for LuxO CDS 4063..5424 /label=LuxO misc_feature 5421..5423 /label=Start of LuxU misc_feature 5763..5765 /label=Stop for LuxU CDS 5421..5765 /label=LuxU misc_feature 4033..5977 /label=BBa_K218001 stem_loop 5997..6040 /label=stem_loop misc_feature 5986..6065 /label=BBa_B0010 terminator 5986..6065 /label=BBa_B0010 stem_loop 6081..6100 /label=T7 TE misc_feature 6107..6107 /label=stop polyA_site 6101..6114 /label=polya misc_feature 6074..6114 /label=BBa_B0012 terminator 6074..6114 /label=BBa_B0012 ORIGIN 1 atgctcgata aaaactaaaa gagcaataga tgaagaaagc gttactattt tccctgattt 61 ctatggtcgg tttttctcca gcgtctcaag caacacaagt tttgaatggg tactggggtt 121 atcaagagtt tttggacgag tttcccgagc aacgaaatct gaccaatgct ttatcagaag 181 cagtacgagc acagccggtc ccactgtcta aaccgacaca acgcccgatt aaaatatcag 241 tggtttaccc aggacagcaa gtttcagatt actgggtaag aaatattgca tcattcgaaa 301 aacgtttgta taagttgaat attaactacc aactgaacca agtgtttact cgtccaaatg 361 ctgatatcaa gcaacaaagc ttgtcattaa tggaagcgct caagagcaaa tcggattact 421 tgattttcac gcttgatacg acaagacacc gtaaatttgt tgagcacgtt ttggactcaa 481 cgaacaccaa attgatcttg caaaatatca ctacaccagt ccgtgagtgg gacaaacatc 541 aaccgttttt atatgtcgga tttgaccacg cagaaggcag tcgtgaatta gcaacagagt 601 tcggaaagtt cttcccaaaa cacacatatt acagtgtgct ctacttttct gaaggttata 661 ttagcgatgt gagaggtgat acttttattc accaagtaaa ccgtgataat aactttgagc 721 tacaatcagc gtattacacg aaggcaacca agcaatccgg ctatgatgct gcgaaagcga 781 gtttagcaaa acatccagat gttgatttta tctatgcatg ttcgaccgac gtagcattag 841 gtgcagtaga cgcactggct gagttgggac gtgaagatat tatgatcaat ggctggggtg 901 gaggctctgc tgagttagac gctatccaga agggtgattt agacatcacc gtcatgcgta 961 tgaatgatga cactggcata gccatggcag aagcgattaa gtgggacttg gaagataaac 1021 cagttccgac cgtatactca ggtgactttg aaatcgtaac aaaggcagat tcaccggaga 1081 gaatcgaagc gctgaaaaag cgcgcattta gatattcaga taattgatga caacaacgcg 1141 atcaaacatt aaaaagcgtc gctcgctggc gacgctcata acaaagatca tcattttagt 1201 tcttgcccca attattctgg ggattttcat tcagagctat tacttctcca agcaaatcat 1261 ttggcaagaa gtagaccgaa ccaaacagca aacctctgca ctgatccaca atatatttga 1321 tagccacttt gcggcgatcc agatacatca tgacagtaat tccaagagcg aagtcattcg 1381 tgatttctac actgatcgcg acacggatgt gctcaacttt ttcttcctca gtatcgacca 1441 aagcgatccg tcgcacacac cagagttccg ttttctaacg gaccacaaag gcatcatttg 1501 ggacgatgga aatgcgcatt tctatggtgt gaacgacctt atccttgata gccttgccaa 1561 tcgggtcagt ttcagtaaca actggtatta cattaatgtc atgacctcca ttggttcgag 1621 acacatgctc gtgcgccgtg tgccgatcct agacccttca acaggagagg tgcttggttt 1681 ctcatttaat gccgtcgtct tagacaacaa cttcgctttg atggaaaagc tcaagagtga 1741 aagtaacgtc gacaatgtgg tgctggttgc taatagcgtt cctttagcaa actctttgat 1801 tggtgatgag ccatataacg ttgctgatgt attgcagcgt aaaagttcag acaaaagact 1861 cgataagctg ttggtaatag aaacgccaat cgtcgtaaat gcagtgacta ccgagctttg 1921 cttgttgacg gtacaagaca atcagagtgt ggtgacatta caaatccaac atattctagc 1981 catgcttgca tcgatcatcg gtatgatcat gattgcctta atgagtaggg aatggattga 2041 gagtaaagtt tcggcgcagt tagaatcttt gatgtcttac acccgctctg ctcgtgagga 2101 aaaagggttt gaacgatttg gcggttcgga tattgaagag tttgatcaca tcggttcaac 2161 ccttgaaagt acattcgaag agcttgaagc gcagaagaag tcgttccgag atctgtttaa 2221 ttttgcctta tcacccatca tggtttggtc tgaagagagt gtcctgattc agatgaaccc 2281 tgccgcgcgc aaagaattag tgatcgaaga cgatcatgaa atcatgcatc cggtcttcca 2341 aggctttaaa gagaaattga ccccacacct caaaatggcg gctcaaggtg cgacgttgac 2401 tggggtgaac gtgcctattg gtaataagat ctaccgatgg aacttgtcgc caattcgtgt 2461 tgatggcgat atcagtggca ttattgtgca aggccaagac attacaacac ttatcgaagc 2521 cgagaagcag agtaacattg cgcgtagaga agcagaaaaa tcggcgcaag cacgtgcaga 2581 cttccttgct aaaatgagcc atgaaattcg tacgccaatc aacggcattt taggtgtcgc 2641 ccaattattg aaagattctg tcgatacaca agagcagaag aatcaaatcg acgtcctgtg 2701 ccacagtggc gagcacttgc ttgcagtact gaacgatatt ctcgatttct caaagataga 2761 gcagggcaag ttcaatattc agaaacaccc gttctccttc accgatacca tgcgtacatt 2821 ggaaaatatt tatcgtccga tttgcacaaa taagggggtg gagttggtca tcgagaatga 2881 gcttgacccg aatgttgaaa tcttcaccga tcaagtccgc ttgaatcaga ttctatttaa 2941 cttagtgagt aatgccgtta agttcacgcc gattggctcg attcgactgc acgcagaact 3001 tgaacaattc tatggtgcgg agaacagcgt gttagttgtg gaactgactg atactggcat 3061 cggcattgaa agcgataagc tcgaccaaat gttcgaacct tttgtgcaag aagagtcgac 3121 aaccacacgc gaatatggcg gtagcggcct aggtttgacc atcgttaaga acctagtcga 3181 tatgttagaa ggtgatgttc aggtccgcag tagcaagggg ggggggacaa catttgttat 3241 aacacttcca gtaaaagatc gtgagcgtgt cttaaggcct ctggaggtca gtcaacgtat 3301 caagccggaa gccttgtttg atgaaagttt aaaagtgcta ctggtggaag ataaccatac 3361 caatgcgttt atccttcagg ctttctgtaa gaagtataaa atgcaggtgg attgggcgaa 3421 agatgggctg gacgcgatgg agctcctttc tgataccacc tacgatctga tcctcatgga 3481 taaccaatta ccccaccttg gtggtattga gaccacgcac gagattcgcc agaacttgag 3541 gcttggaacg ccaatttacg cgtgtacagc agacaccgcg aaagaaacca gtgatgcgtt 3601 tatggcggca ggtgcaaact atgtcatgct gaagccaatt aaagagaatg cgttacatga 3661 ggcgtttgtc gatttcaaac aacgtttctt ggtagaaaga acctaacggt ttaatggcag 3721 tgataagtta ggggctgaag ttaaataata aaagtaaaga agggagcgta atgcactaat 3781 gccgttcact taaggtgatc ggcattgttt tttctagggt atcggtacta gagccaggca 3841 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 3901 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 3961 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga 4021 gcactactag agacagaagg tcaaaagtct cgttatctac ttatggtaga agacaccgca 4081 tccgttgcgg cactttaccg ctcttacctc acgccacttg gcatcgatat caatattgtt 4141 ggaacaggca gagacgccat tgaaagcctg aaccatcgca ttcctgatct tattctgctc 4201 gatcttcgtc tacctgatat gacggggatg gacgtattgc acgcggtgaa gaaaagccac 4261 ccagacgtgc caatcatctt catgacagcc catggttcta tcgatactgc ggtagaggcg 4321 atgcgccacg gttctcaaga cttcctaatc aaaccatgtg aagcagaccg tttacgtgtc 4381 acggtgaaca atgcgatccg taaagcaacc aaattaaaga atgaagctga caaccccggt 4441 aaccaaaatt accaaggctt catcggcagt agccaaacga tgcagcaggt ttaccgcacc 4501 attgactcgg cagcgagcag taaagcgagt attttcatca cgggtgaaag tggtacgggt 4561 aaagaagtgt gtgccgaagc gattcacgca gcaagcaaac gcggtgataa gccgtttatc 4621 gccatcaact gtgcggcaat cccgaaagac cttattgaaa gtgagctgtt tggtcacgta 4681 aaaggtgcgt ttactggtgc tgcgaatgac cgacaaggtg cggcagagct tgctgatggc 4741 ggcaccttgt tccttgatga actctgtgaa atggacttgg atcttcaaac taagctattg 4801 cgctttatcc aaacgggtac attccaaaaa gtcggttctt ctaaaatgaa gagcgtggat 4861 gtgcgctttg tgtgtgcaac taaccgagac ccttggaaag aagtgcaaga aggccgtttc 4921 cgtgaagact tgtattaccg tttgtacgtg attcctttgc accttccgcc gctgcgtgag 4981 cgtggtaaag acgttattga aattgcatac tcgttgcttg gttatatgtc tcatgaggaa 5041 ggtaagagtt tcgtccgttt cgcacaagac gtgattgaaa gattcaacag ctacgaatgg 5101 ccgggtaacg ttcgccagtt gcaaaacgta ttgcgtaata tcgtggtact gaacaatggc 5161 aaagagatca cgctggatat gttaccgcca ccactgaatc agcctgttgt gcgccaatcg 5221 gtagcaaaat tcattgaacc tgacattatg acggtgtcag atattatgcc gctttggatg 5281 acagagaaaa tggctattga gcaggcaatt caagcgtgtg aaggcaacat tccacgcgct 5341 gctggctatt tggatgttag tccatcaacg atttatcgca agttgcaagc ttggaatagc 5401 aaggacgaaa aacaaaacgt atgaatacgg acgtattaaa tcagcaaaaa attgaagaac 5461 tgtctgcgga aattggtagc gataatgttc ctgttttgct tgatattttt cttggggaaa 5521 tggactccta cattggcact ttaactgaac ttcagggctc agagcagctg ttgtatttaa 5581 aagagatcag ccacgcactg aaaagtagtg ctgccagctt tggcgcagat cgattgtgtg 5641 aacgagcgat tgccatcgac aagaaagcaa aagcgaatca attgcaagag caggggatgg 5701 agacgagcga aatgctcgct ttacttcata tcactcgtga cgcctaccgt tcttggacaa 5761 actaacgttt cgagcaagac attaagcgca acgtaaaaac acaaagccct tccggtgtgg 5821 aagggctttt ttgtttgggg agtttgctcc gaatcgaagc cgctttctca atgcttttcg 5881 tctagttaga cagtaagcgc tccataaaac ccgcattcta atcgcctagc gcgaagaata 5941 agatcaagtc tccaaccatg aggagatttg aaatgggtac tagagccagg catcaaataa 6001 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 6061 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //