BBa_K218006
type: ComponentDefinition
persistent identity: BBa K218006
display id: BBa_K218006
version: 1
was generated by: iGEM to SBOL conversion
description: LuxPQ followed by terminator and LuxOU generator
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: source
owned by: james
owned by: myers
mutable description: description
discontinued: false
dominant: true
experience: experience/None
group u list: _321_
m user id: 0
owner id: 4333
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: design
star: false
creator: Jeremy Kubik
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K218000
component: BBa_B0012
component: BBa_B0010
component: BBa_K218001
component: BBa_R0040
sequence annotation: BBa_K218000
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_K218001
sequence: https://synbiohub.org/public/igem/BBa_K218006_sequence/1

BBa_K218000
type: Component
persistent identity: component2053818
display id: component2053818
version: 1
top level: BBa_K218006
definition: BBa_K218000
access: public

BBa_B0012
type: Component
persistent identity: component2053821
display id: component2053821
version: 1
top level: BBa_K218006
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2053819
display id: component2053819
version: 1
top level: BBa_K218006
definition: BBa_B0010
access: public

BBa_K218001
type: Component
persistent identity: component2053837
display id: component2053837
version: 1
top level: BBa_K218006
definition: BBa_K218001
access: public

BBa_R0040
type: Component
persistent identity: component2053825
display id: component2053825
version: 1
top level: BBa_K218006
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component2053840
display id: component2053840
version: 1
top level: BBa_K218006
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2053838
display id: component2053838
version: 1
top level: BBa_K218006
definition: BBa_B0010
access: public

BBa_K218000
type: SequenceAnnotation
persistent identity: annotation2053818
display id: annotation2053818
version: 1
top level: BBa_K218006
location: https://synbiohub.org/public/igem/BBa_K218006/annotation2053818/range2053818/1
component: BBa_K218000

https://synbiohub.org/public/igem/BBa_K218006/annotation2053818/range2053818/1
type: Range
persistent identity: range2053818
display id: range2053818
version: 1
top level: BBa_K218006
direction: direction/reverse
start: 1
end: 3825
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053819
display id: annotation2053819
version: 1
top level: BBa_K218006
location: https://synbiohub.org/public/igem/BBa_K218006/annotation2053819/range2053819/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K218006/annotation2053819/range2053819/1
type: Range
persistent identity: range2053819
display id: range2053819
version: 1
top level: BBa_K218006
direction: direction/forward
start: 3834
end: 3913
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053821
display id: annotation2053821
version: 1
top level: BBa_K218006
location: https://synbiohub.org/public/igem/BBa_K218006/annotation2053821/range2053821/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K218006/annotation2053821/range2053821/1
type: Range
persistent identity: range2053821
display id: range2053821
version: 1
top level: BBa_K218006
direction: direction/forward
start: 3922
end: 3962
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2053825
display id: annotation2053825
version: 1
top level: BBa_K218006
location: https://synbiohub.org/public/igem/BBa_K218006/annotation2053825/range2053825/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K218006/annotation2053825/range2053825/1
type: Range
persistent identity: range2053825
display id: range2053825
version: 1
top level: BBa_K218006
direction: direction/forward
start: 3971
end: 4024
orientation: inline

BBa_K218001
type: SequenceAnnotation
persistent identity: annotation2053837
display id: annotation2053837
version: 1
top level: BBa_K218006
location: https://synbiohub.org/public/igem/BBa_K218006/annotation2053837/range2053837/1
component: BBa_K218001

https://synbiohub.org/public/igem/BBa_K218006/annotation2053837/range2053837/1
type: Range
persistent identity: range2053837
display id: range2053837
version: 1
top level: BBa_K218006
direction: direction/reverse
start: 4033
end: 5977
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053838
display id: annotation2053838
version: 1
top level: BBa_K218006
location: https://synbiohub.org/public/igem/BBa_K218006/annotation2053838/range2053838/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K218006/annotation2053838/range2053838/1
type: Range
persistent identity: range2053838
display id: range2053838
version: 1
top level: BBa_K218006
direction: direction/forward
start: 5986
end: 6065
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053840
display id: annotation2053840
version: 1
top level: BBa_K218006
location: https://synbiohub.org/public/igem/BBa_K218006/annotation2053840/range2053840/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K218006/annotation2053840/range2053840/1
type: Range
persistent identity: range2053840
display id: range2053840
version: 1
top level: BBa_K218006
direction: direction/forward
start: 6074
end: 6114
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K218000
type: ComponentDefinition
persistent identity: BBa K218000
display id: BBa_K218000
version: 1
was generated by: iGEM to SBOL conversion
description: LuxPQ from Vibrio harveyi
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: GenBank Accession: NC_009784 Vibrio harveyi ATCC BAA-1116 chromosome II, REGION: 579654..580751. Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM. Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing. Cell. 2006 Sep 22;126(6):1095-108.
owned by: james
owned by: myers
mutable description: LuxP is a ABC-type sugar transport system periplasmic protein (Protein ID = YP_001447483.1). LuxQ is a histidine kinase (Protein ID = YP_001447484.1). Usage and Biology Quorum-sensing bacteria produce and release chemical signal molecules termed autoinducers (AIs) whose external concentration increases as a function of increasing cell-population density. Bacteria detect the accumulation of a minimal threshold stimulatory concentration of these autoinducers and alter gene expression, and therefore their behavior. Using these signal-response systems, bacteria synchronize particular behaviors on a population-wide scale and thus function as multicellular organisms. The bioluminescent marine bacterium Vibrio harveyi uses three different AIs???AHL, CAI-1, and AI-2???to control the expression of genes responsible for bioluminescence and numerous other traits. We have designed our System 2 based on V. harveyi AI-2 signaling. V. harveyi AI-2 signal is a furanosyl borate diester, production of which requires the LuxS enzyme. Biosynthesis of AI-2 is dependent on the usage of S-adenosylmethionine (SAM) by the cell in various methylation reactions. For this reason, during periods of exponential growth, there is a very large production of AI-2, thus perhaps signaling to neighbors that a suitable environment for growth (i.e. rich in nutrients) has been found. LuxS catalyzes the formation of the (S)-4,5-dihydroxy-2,3-pentanedione (DPD) intermediate which spontaneously cyclizes and reacts with borate to give AI-2. AI-2 is bound in the periplasm by the protein LuxP, which is constitutively bound to LuxQ, a membrane bound histidine kinase sensor. The binding of AI-2 to LuxP is necessary in regulating the activity of the periplasm-bound LuxQ. At low cell density, in the absence of significant amounts of autoinducers, LuxQ acts as a kinase, autophosphorylates, and subsequently transfers the phosphate to the cytoplasmic protein LuxU. LuxU passes the phosphate to the DNA-binding response …
discontinued: false
dominant: false
group u list: _321_
m user id: 0
owner id: 4333
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part was submitted to the Registry in 2008 (K131015); however, this part was cloned again and sequenced and is being re-submitted. LuxP and LuxQ are found in the operon, thus we decided to keep them together. We kept natural RBS in front of LuxP, as well as the intrinsic terminator. Silent mutations were performed on a EcoRI site located within LuxP, EcorI site within LuxQ, and XbaI site within LuxQ.
star: false
creator: Jeremy Kubik
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: start of LuxP
sequence annotation: LuxP
sequence annotation: Silent mutation to remove EcoRI site
sequence annotation: stop for LuxP
sequence annotation: start of LuxQ
sequence annotation: LuxQ
sequence annotation: Silent mutation to remove XbaI site
sequence annotation: stop for LuxQ
sequence: https://synbiohub.org/public/igem/BBa_K218000_sequence/1

start of LuxP
type: SequenceAnnotation
persistent identity: annotation2048298
display id: annotation2048298
version: 1
top level: BBa_K218000
location: https://synbiohub.org/public/igem/BBa_K218000/annotation2048298/range2048298/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K218000/annotation2048298/range2048298/1
type: Range
persistent identity: range2048298
display id: range2048298
version: 1
top level: BBa_K218000
direction: direction/forward
start: 30
end: 32
orientation: inline

LuxP
type: SequenceAnnotation
persistent identity: annotation2048320
display id: annotation2048320
version: 1
top level: BBa_K218000
location: https://synbiohub.org/public/igem/BBa_K218000/annotation2048320/range2048320/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K218000/annotation2048320/range2048320/1
type: Range
persistent identity: range2048320
display id: range2048320
version: 1
top level: BBa_K218000
direction: direction/forward
start: 30
end: 1127
orientation: inline

Silent mutation to remove EcoRI site
type: SequenceAnnotation
persistent identity: annotation2048335
display id: annotation2048335
version: 1
top level: BBa_K218000
location: https://synbiohub.org/public/igem/BBa_K218000/annotation2048335/range2048335/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K218000/annotation2048335/range2048335/1
type: Range
persistent identity: range2048335
display id: range2048335
version: 1
top level: BBa_K218000
direction: direction/forward
start: 599
end: 599
orientation: inline

stop for LuxP
type: SequenceAnnotation
persistent identity: annotation2048319
display id: annotation2048319
version: 1
top level: BBa_K218000
location: https://synbiohub.org/public/igem/BBa_K218000/annotation2048319/range2048319/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K218000/annotation2048319/range2048319/1
type: Range
persistent identity: range2048319
display id: range2048319
version: 1
top level: BBa_K218000
direction: direction/forward
start: 1125
end: 1127
orientation: inline

start of LuxQ
type: SequenceAnnotation
persistent identity: annotation2048299
display id: annotation2048299
version: 1
top level: BBa_K218000
location: https://synbiohub.org/public/igem/BBa_K218000/annotation2048299/range2048299/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K218000/annotation2048299/range2048299/1
type: Range
persistent identity: range2048299
display id: range2048299
version: 1
top level: BBa_K218000
direction: direction/forward
start: 1127
end: 1129
orientation: inline

LuxQ
type: SequenceAnnotation
persistent identity: annotation2048322
display id: annotation2048322
version: 1
top level: BBa_K218000
location: https://synbiohub.org/public/igem/BBa_K218000/annotation2048322/range2048322/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K218000/annotation2048322/range2048322/1
type: Range
persistent identity: range2048322
display id: range2048322
version: 1
top level: BBa_K218000
direction: direction/forward
start: 1127
end: 3706
orientation: inline

Silent mutation to remove EcoRI site
type: SequenceAnnotation
persistent identity: annotation2048336
display id: annotation2048336
version: 1
top level: BBa_K218000
location: https://synbiohub.org/public/igem/BBa_K218000/annotation2048336/range2048336/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K218000/annotation2048336/range2048336/1
type: Range
persistent identity: range2048336
display id: range2048336
version: 1
top level: BBa_K218000
direction: direction/forward
start: 1465
end: 1465
orientation: inline

Silent mutation to remove XbaI site
type: SequenceAnnotation
persistent identity: annotation2048337
display id: annotation2048337
version: 1
top level: BBa_K218000
location: https://synbiohub.org/public/igem/BBa_K218000/annotation2048337/range2048337/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K218000/annotation2048337/range2048337/1
type: Range
persistent identity: range2048337
display id: range2048337
version: 1
top level: BBa_K218000
direction: direction/forward
start: 1618
end: 1618
orientation: inline

stop for LuxQ
type: SequenceAnnotation
persistent identity: annotation2048321
display id: annotation2048321
version: 1
top level: BBa_K218000
location: https://synbiohub.org/public/igem/BBa_K218000/annotation2048321/range2048321/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K218000/annotation2048321/range2048321/1
type: Range
persistent identity: range2048321
display id: range2048321
version: 1
top level: BBa_K218000
direction: direction/forward
start: 3704
end: 3706
orientation: inline

BBa_K218001
type: ComponentDefinition
persistent identity: BBa K218001
display id: BBa_K218001
version: 1
was generated by: iGEM to SBOL conversion
description: LuxOU from Vibrio harveyi
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2 Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.
owned by: james
owned by: myers
mutable description: Vibrio harveyi luminescence regulatory protein LuxO (GenBank accession# P0C5S5) and phosphorelay protein LuxU (GenBank accession# AAD12737). Quorum-sensing bacteria produce and release chemical signal molecules termed autoinducers (AIs) whose external concentration increases as a function of increasing cell-population density. Bacteria detect the accumulation of a minimal threshold stimulatory concentration of these autoinducers and alter gene expression, and therefore their behavior. Using these signal-response systems, bacteria synchronize particular behaviors on a population-wide scale and thus function as multicellular organisms. The bioluminescent marine bacterium Vibrio harveyi uses three different AIs???AHL, CAI-1, and AI-2???to control the expression of genes responsible for bioluminescence and numerous other traits. We have designed our System 2 based on V. harveyi AI-2 signaling. V. harveyi AI-2 signal is a furanosyl borate diester, production of which requires the LuxS enzyme. Biosynthesis of AI-2 is dependent on the usage of S-adenosylmethionine (SAM) by the cell in various methylation reactions. For this reason, during periods of exponential growth, there is a very large production of AI-2, thus perhaps signaling to neighbors that a suitable environment for growth (i.e. rich in nutrients) has been found. LuxS catalyzes the formation of the (S)-4,5-dihydroxy-2,3-pentanedione (DPD) intermediate which spontaneously cyclizes and reacts with borate to give AI-2. AI-2 is bound in the periplasm by the protein LuxP, which is constitutively bound to LuxQ, a membrane bound histidine kinase sensor. The binding of AI-2 to LuxP is necessary in regulating the activity of the periplasm-bound LuxQ. At low cell density, in the absence of significant amounts of autoinducers, LuxQ acts as a kinase, autophosphorylates, and subsequently transfers the phosphate to the cytoplasmic protein LuxU. LuxU passes the phosphate to the DNA-binding response regulator protein LuxO. Phos…
discontinued: false
dominant: false
group u list: _321_
m user id: 0
owner id: 4333
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part was submitted to the Registry in 2008 (K131016); however, this part was cloned again and sequenced and is being re-submitted. LuxO and LuxU are found within the operon, therefore we have decided to keep these genes together. The internal RBS was also kept.
star: false
creator: Jeremy Kubik
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: RBS
sequence annotation: Start of LuxO
sequence annotation: LuxO
sequence annotation: Start of LuxU
sequence annotation: LuxU
sequence annotation: Stop for LuxO
sequence annotation: Stop for LuxU
sequence: https://synbiohub.org/public/igem/BBa_K218001_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2048769
display id: annotation2048769
version: 1
top level: BBa_K218001
location: https://synbiohub.org/public/igem/BBa_K218001/annotation2048769/range2048769/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K218001/annotation2048769/range2048769/1
type: Range
persistent identity: range2048769
display id: range2048769
version: 1
top level: BBa_K218001
direction: direction/forward
start: 5
end: 12
orientation: inline

Start of LuxO
type: SequenceAnnotation
persistent identity: annotation2048770
display id: annotation2048770
version: 1
top level: BBa_K218001
location: https://synbiohub.org/public/igem/BBa_K218001/annotation2048770/range2048770/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K218001/annotation2048770/range2048770/1
type: Range
persistent identity: range2048770
display id: range2048770
version: 1
top level: BBa_K218001
direction: direction/forward
start: 31
end: 33
orientation: inline

LuxO
type: SequenceAnnotation
persistent identity: annotation2048773
display id: annotation2048773
version: 1
top level: BBa_K218001
location: https://synbiohub.org/public/igem/BBa_K218001/annotation2048773/range2048773/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K218001/annotation2048773/range2048773/1
type: Range
persistent identity: range2048773
display id: range2048773
version: 1
top level: BBa_K218001
direction: direction/forward
start: 31
end: 1392
orientation: inline

Start of LuxU
type: SequenceAnnotation
persistent identity: annotation2048774
display id: annotation2048774
version: 1
top level: BBa_K218001
location: https://synbiohub.org/public/igem/BBa_K218001/annotation2048774/range2048774/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K218001/annotation2048774/range2048774/1
type: Range
persistent identity: range2048774
display id: range2048774
version: 1
top level: BBa_K218001
direction: direction/forward
start: 1389
end: 1391
orientation: inline

LuxU
type: SequenceAnnotation
persistent identity: annotation2048776
display id: annotation2048776
version: 1
top level: BBa_K218001
location: https://synbiohub.org/public/igem/BBa_K218001/annotation2048776/range2048776/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K218001/annotation2048776/range2048776/1
type: Range
persistent identity: range2048776
display id: range2048776
version: 1
top level: BBa_K218001
direction: direction/forward
start: 1389
end: 1733
orientation: inline

Stop for LuxO
type: SequenceAnnotation
persistent identity: annotation2048771
display id: annotation2048771
version: 1
top level: BBa_K218001
location: https://synbiohub.org/public/igem/BBa_K218001/annotation2048771/range2048771/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K218001/annotation2048771/range2048771/1
type: Range
persistent identity: range2048771
display id: range2048771
version: 1
top level: BBa_K218001
direction: direction/forward
start: 1390
end: 1392
orientation: inline

Stop for LuxU
type: SequenceAnnotation
persistent identity: annotation2048775
display id: annotation2048775
version: 1
top level: BBa_K218001
location: https://synbiohub.org/public/igem/BBa_K218001/annotation2048775/range2048775/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K218001/annotation2048775/range2048775/1
type: Range
persistent identity: range2048775
display id: range2048775
version: 1
top level: BBa_K218001
direction: direction/forward
start: 1731
end: 1733
orientation: inline

https://synbiohub.org/public/igem/BBa_K218006_sequence/1
type: Sequence
persistent identity: BBa K218006 sequence
display id: BBa_K218006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctcgataaaaactaaaagagcaatagatgaagaaagcgttactattttccctgatttctatggtcggtttttctccagcgtctcaagcaacacaagttttgaatgggtactggggttatcaagagtttttggacgagtttcccgagcaacgaaatctgaccaatgctttatcagaagcagtacgagcacagccggtcccactgtctaaaccgacacaacgcccgattaaaatatcagtggtttacccaggacagcaagtttcagattactgggtaagaaatattgcatcattcgaaaaacgtttgtataagttgaatattaactaccaactgaaccaagtgtttactcgtccaaatgctgatatcaagcaacaaagcttgtcattaatggaagcgctcaagagcaaatcggattacttgattttcacgcttgatacgacaagacaccgtaaatttgttgagcacgttttggactcaacgaacaccaaattgatcttgcaaaatatcactacaccagtccgtgagtgggacaaacatcaaccgtttttatatgtcggatttgaccacgcagaaggcagtcgtgaattagcaacagagttcggaaagttcttcccaaaacacacatattacagtgtgctctacttttctgaaggttatattagcgatgtgagaggtgatacttttattcaccaagtaaaccgtgataataactttgagctacaatcagcgtattacacgaaggcaaccaagcaatccggctatgatgctgcgaaagcgagtttagcaaaacatccagatgttgattttatctatgcatgttcgaccgacgtagcattaggtgcagtagacgcactggctgagttgggacgtgaagatattatgatcaatggctggggtggaggctctgctgagttagacgctatccagaagggtgatttagacatcaccgtcatgcgtatgaatgatgacactggcatagccatggcagaagcgattaagtgggacttggaagataaaccagttccgaccgtatactcaggtgactttgaaatcgtaacaaaggcagattcaccggagagaatcgaagcgctgaaaaagcgcgcatttagatattcagataattgatgacaacaacgcgatcaaacattaaaaagcgtcgctcgctggcgacgctcataacaaagatcatcattttagttcttgccccaattattctggggattttcattcagagctattacttctccaagcaaatcatttggcaagaagtagaccgaaccaaacagcaaacctctgcactgatccacaatatatttgatagccactttgcggcgatccagatacatcatgacagtaattccaagagcgaagtcattcgtgatttctacactgatcgcgacacggatgtgctcaactttttcttcctcagtatcgaccaaagcgatccgtcgcacacaccagagttccgttttctaacggaccacaaaggcatcatttgggacgatggaaatgcgcatttctatggtgtgaacgaccttatccttgatagccttgccaatcgggtcagtttcagtaacaactggtattacattaatgtcatgacctccattggttcgagacacatgctcgtgcgccgtgtgccgatcctagacccttcaacaggagaggtgcttggtttctcatttaatgccgtcgtcttagacaacaacttcgctttgatggaaaagctcaagagtgaaagtaacgtcgacaatgtggtgctggttgctaatagcgttcctttagcaaactctttgattggtgatgagccatataacgttgctgatgtattgcagcgtaaaagttcagacaaaagactcgataagctgttggtaatagaaacgccaatcgtcgtaaatgcagtgactaccgagctttgcttgttgacggtacaagacaatcagagtgtggtgacattacaaatccaacatattctagccatgcttgcatcgatcatcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K218000_sequence/1
type: Sequence
persistent identity: BBa K218000 sequence
display id: BBa_K218000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctcgataaaaactaaaagagcaatagatgaagaaagcgttactattttccctgatttctatggtcggtttttctccagcgtctcaagcaacacaagttttgaatgggtactggggttatcaagagtttttggacgagtttcccgagcaacgaaatctgaccaatgctttatcagaagcagtacgagcacagccggtcccactgtctaaaccgacacaacgcccgattaaaatatcagtggtttacccaggacagcaagtttcagattactgggtaagaaatattgcatcattcgaaaaacgtttgtataagttgaatattaactaccaactgaaccaagtgtttactcgtccaaatgctgatatcaagcaacaaagcttgtcattaatggaagcgctcaagagcaaatcggattacttgattttcacgcttgatacgacaagacaccgtaaatttgttgagcacgttttggactcaacgaacaccaaattgatcttgcaaaatatcactacaccagtccgtgagtgggacaaacatcaaccgtttttatatgtcggatttgaccacgcagaaggcagtcgtgaattagcaacagagttcggaaagttcttcccaaaacacacatattacagtgtgctctacttttctgaaggttatattagcgatgtgagaggtgatacttttattcaccaagtaaaccgtgataataactttgagctacaatcagcgtattacacgaaggcaaccaagcaatccggctatgatgctgcgaaagcgagtttagcaaaacatccagatgttgattttatctatgcatgttcgaccgacgtagcattaggtgcagtagacgcactggctgagttgggacgtgaagatattatgatcaatggctggggtggaggctctgctgagttagacgctatccagaagggtgatttagacatcaccgtcatgcgtatgaatgatgacactggcatagccatggcagaagcgattaagtgggacttggaagataaaccagttccgaccgtatactcaggtgactttgaaatcgtaacaaaggcagattcaccggagagaatcgaagcgctgaaaaagcgcgcatttagatattcagataattgatgacaacaacgcgatcaaacattaaaaagcgtcgctcgctggcgacgctcataacaaagatcatcattttagttcttgccccaattattctggggattttcattcagagctattacttctccaagcaaatcatttggcaagaagtagaccgaaccaaacagcaaacctctgcactgatccacaatatatttgatagccactttgcggcgatccagatacatcatgacagtaattccaagagcgaagtcattcgtgatttctacactgatcgcgacacggatgtgctcaactttttcttcctcagtatcgaccaaagcgatccgtcgcacacaccagagttccgttttctaacggaccacaaaggcatcatttgggacgatggaaatgcgcatttctatggtgtgaacgaccttatccttgatagccttgccaatcgggtcagtttcagtaacaactggtattacattaatgtcatgacctccattggttcgagacacatgctcgtgcgccgtgtgccgatcctagacccttcaacaggagaggtgcttggtttctcatttaatgccgtcgtcttagacaacaacttcgctttgatggaaaagctcaagagtgaaagtaacgtcgacaatgtggtgctggttgctaatagcgttcctttagcaaactctttgattggtgatgagccatataacgttgctgatgtattgcagcgtaaaagttcagacaaaagactcgataagctgttggtaatagaaacgccaatcgtcgtaaatgcagtgactaccgagctttgcttgttgacggtacaagacaatcagagtgtggtgacattacaaatccaacatattctagccatgcttgcatcgatcatcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K218001_sequence/1
type: Sequence
persistent identity: BBa K218001 sequence
display id: BBa_K218001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acagaaggtcaaaagtctcgttatctacttatggtagaagacaccgcatccgttgcggcactttaccgctcttacctcacgccacttggcatcgatatcaatattgttggaacaggcagagacgccattgaaagcctgaaccatcgcattcctgatcttattctgctcgatcttcgtctacctgatatgacggggatggacgtattgcacgcggtgaagaaaagccacccagacgtgccaatcatcttcatgacagcccatggttctatcgatactgcggtagaggcgatgcgccacggttctcaagacttcctaatcaaaccatgtgaagcagaccgtttacgtgtcacggtgaacaatgcgatccgtaaagcaaccaaattaaagaatgaagctgacaaccccggtaaccaaaattaccaaggcttcatcggcagtagccaaacgatgcagcaggtttaccgcaccattgactcggcagcgagcagtaaagcgagtattttcatcacgggtgaaagtggtacgggtaaagaagtgtgtgccgaagcgattcacgcagcaagcaaacgcggtgataagccgtttatcgccatcaactgtgcggcaatcccgaaagaccttattgaaagtgagctgtttggtcacgtaaaaggtgcgtttactggtgctgcgaatgaccgacaaggtgcggcagagcttgctgatggcggcaccttgttccttgatgaactctgtgaaatggacttggatcttcaaactaagctattgcgctttatccaaacgggtacattccaaaaagtcggttcttctaaaatgaagagcgtggatgtgcgctttgtgtgtgcaactaaccgagacccttggaaagaagtgcaagaaggccgtttccgtgaagacttgtattaccgtttgtacgtgattcctttgcaccttccgccgctgcgtgagcgtggtaaagacgttattgaaattgcatactcgttgcttggttatatgtctcatgaggaaggtaagagtttcgtccgtttcgcacaagacgtgattgaaagattcaacagctacgaatggccgggtaacgttcgccagttgcaaaacgtattgcgtaatatcgtggtactgaacaatggcaaagagatcacgctggatatgttaccgccaccactgaatcagcctgttgtgcgccaatcggtagcaaaattcattgaacctgacattatgacggtgtcagatattatgccgctttggatgacagagaaaatggctattgagcaggcaattcaagcgtgtgaaggcaacattccacgcgctgctggctatttggatgttagtccatcaacgatttatcgcaagttgcaagcttggaatagcaaggacgaaaaacaaaacgtatgaatacggacgtattaaatcagcaaaaaattgaagaactgtctgcggaaattggtagcgataatgttcctgttttgcttgatatttttcttggggaaatggactcctacattggcactttaactgaacttcagggctcagagcagctgttgtatttaaaagagatcagccacgcactgaaaagtagtgctgccagctttggcgcagatcgattgtgtgaacgagcgattgccatcgacaagaaagcaaaagcgaatcaattgcaagagcaggggatggagacgagcgaaatgctcgctttacttcatatcactcgtgacgcctaccgttcttggacaaactaacgtttcgagcaagacattaagcgcaacgtaaaaacacaaagcccttccggtgtggaagggcttttttgtttggggagtttgctccgaatcgaagccgctttctcaatgcttttcgtctagttagacagtaagcgctccataaaacccgcattctaatcgcctagcgcgaagaataagatcaagtctccaaccatgaggagatttgaaatggg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z