##gff-version 3
##sequence-region BBa_K218021 1 1579
BBa_K218021	.	CDS	81	1442	.	+	0	ID=BBa_K218019;Name=BBa_K218019
BBa_K218021	.	sequence_alteration	219	221	.	+	0	ID=annotation2061377;Name=Mutation;Parent=BBa_K218019
BBa_K218021	.	start_codon	81	83	.	+	0	ID=annotation2061347;Name=Start;Parent=BBa_K218019
BBa_K218021	.	stop_codon	1440	1442	.	+	0	ID=annotation2061375;Name=Stop;Parent=BBa_K218019
BBa_K218021	.	terminator	1451	1530	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K218021	.	stem_loop	1462	1505	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K218021	.	engineered_region	1451	1530	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K218021	.	terminator	1539	1579	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K218021	.	engineered_region	1539	1579	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K218021	.	polyA_site	1566	1579	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K218021	.	stop_codon	1572	1572	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K218021	.	stem_loop	1546	1565	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K218021	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K218021	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K218021	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K218021	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K218021	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K218021	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K218021	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K218021	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K218021
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtagaagacaccgcatccgttgcggcactttaccgctcttacctcacgccacttggcatcgatatcaatattgtt
ggaacaggcagagacgccattgaaagcctgaaccatcgcattcctgatcttattctgctcgcgctccgtctacctgata
tgacggggatggacgtattgcacgcggtgaagaaaagccacccagacgtgccaatcatcttcatgacagcccatggttc
tatcgatactgcggtagaggcgatgcgccacggttctcaagacttcctaatcaaaccatgtgaagcagaccgtttacgt
gtcacggtgaacaatgcgatccgtaaagcaaccaaattaaagaatgaagctgacaaccccggtaaccaaaattaccaag
gcttcatcggcagtagccaaacgatgcagcaggtttaccgcaccattgactcggcagcgagcagtaaagcgagtatttt
catcacgggtgaaagtggtacgggtaaagaagtgtgtgccgaagcgattcacgcagcaagcaaacgcggtgataagccg
tttatcgccatcaactgtgcggcaatcccgaaagaccttattgaaagtgagctgtttggtcacgtaaaaggtgcgttta
ctggtgctgcgaatgaccgacaaggtgcggcagagcttgctgatggcggcaccttgttccttgatgaactctgtgaaat
ggacttggatcttcaaactaagctattgcgctttatccaaacgggtacattccaaaaagtcggttcttctaaaatgaag
agcgtggatgtgcgctttgtgtgtgcaactaaccgagacccttggaaagaagtgcaagaaggccgtttccgtgaagact
tgtattaccgtttgtacgtgattcctttgcaccttccgccgctgcgtgagcgtggtaaagacgttattgaaattgcata
ctcgttgcttggttatatgtctcatgaggaaggtaagagtttcgtccgtttcgcacaagacgtgattgaaagattcaac
agctacgaatggccgggtaacgttcgccagttgcaaaacgtattgcgtaatatcgtggtactgaacaatggcaaagaga
tcacgctggatatgttaccgccaccactgaatcagcctgttgtgcgccaatcggtagcaaaattcattgaacctgacat
tatgacggtgtcagatattatgccgctttggatgacagagaaaatggctattgagcaggcaattcaagcgtgtgaaggc
aacattccacgcgctgctggctatttggatgttagtccatcaacgatttatcgcaagttgcaagcttggaatagcaagg
acgaaaaacaaaacgtatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata