LOCUS       BBa_K2182003            3166 bp    DNA     linear       23-Dec-2024
DEFINITION  Translational Unit for His-tagged NOX
ACCESSION   BBa_K2182003
VERSION     BBa_K2182003.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..65
                     /label=RBS
     RBS             44..1625
                     /label=BBa_K2182004
     misc_feature    1632..1652
                     /label=His tag
     CDS             1653..3029
                     /label=Nox
     CDS             1632..3029
                     /label=BBa_K2182001
     stem_loop       3049..3092
                     /label=stem_loop
     misc_feature    3038..3117
                     /label=BBa_B0010
     terminator      3038..3117
                     /label=BBa_B0010
     stem_loop       3133..3152
                     /label=T7 TE
     misc_feature    3159..3159
                     /label=stop
     polyA_site      3153..3166
                     /label=polya
     misc_feature    3126..3166
                     /label=BBa_B0012
     terminator      3126..3166
                     /label=BBa_B0012
     terminator      3038..3166
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gaggacggct agctcagtcc
       61 taggtacagt gctagcttac ctggaaagag agccatccat gcaccatcac catcaccata
      121 tgagtaaaat cgttgtagtt ggtgcaaacc acgcaggtac agcgtgtatt aatacaatgt
      181 tggacaatta tggtgcagaa aacgaagtag ttatttttga ccaaaactca aacatttcat
      241 tcttggcatg cggaatggcc ctttggattg gacagcaaat cagcaagcca gatggacttt
      301 tttatgcaga taaagaaact tttgaagcaa aaggcgcaaa agtttatatg aactcaccag
      361 ttgagtcaat tgattatgat gcgaaaaaag ttacagctat tgttgatggc aaagagcatg
      421 tagagtcata tgacaaattg attttggcaa caggttctca gccaattctg cctccgatta
      481 aaggtgctga gatggatcct aacagtcgtg aatttaagtc aaccttggaa aatctgcagt
      541 ttgttaaatt gtatcaaaat gctgctgatg ttatcgagaa attgcaagac aagagcaaac
      601 atatcgaacg tgtggcagta gtaggagctg gttacattgg ggttgagtta gcagaagcct
      661 tcaaacgcct tggtaaagaa gtgattctga ttgacgttgt ggatacttgc ttggcgggtt
      721 actatgacca cgacttgtct gaaatgatgc gtcaaaatct tgaagacaat ggtgttcaat
      781 tagcctttgg ccaaactgtt caagctattg aaggtgaaaa caaggtagaa cgcatagtaa
      841 cggataaagc tagctatgat gtagatatgg tcgtcttggc agttggcttc cgtccaaaca
      901 ctggtcttgg tgctggcaag ttggaaacat tccgcaatgg tgctttcttg gtggataaga
      961 agcaagaaac tagcattaaa gatgtttatg caatcggtga ctgtgcgact gtctacgata
     1021 actctatcaa tgatacaaac tacatcgcct tggcatctaa cgccctgcgc tctggtattg
     1081 tagcagctca taatgcttgc ggtcatgaat tggagtcaaa cggtgttcaa ggttctaacg
     1141 gtatcgaaat ctttggtttg aagatggttt caactggtct gactgaagaa aaagctaaac
     1201 gctttggtta cagcccagct gtagttgagt ttaaagacac tcaaaaacca actttccttg
     1261 aaaaggttga gcatcatgat gttacaatta agattgtcta tgataaggat acacgtgtag
     1321 ttcttggagc tcagatggtt tctagagaag atatgtctat gggtattcac atgttctcat
     1381 tggctattca ggaaaaggtt acaattgata gattggcctt gctggatctt ttcttcctgc
     1441 cacacttcaa caaaccgtac aactacatta ctcaagcagc tttgaaagca aaataaccag
     1501 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1561 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1621 ttatatacta gatgcaccat caccatcacc atatgagtaa aatcgttgta gttggtgcaa
     1681 accacgcagg tacagcgtgt attaatacaa tgttggacaa ttatggtgca gaaaacgaag
     1741 tagttatttt tgaccaaaac tcaaacattt cattcttggc atgcggaatg gccctttgga
     1801 ttggacagca aatcagcaag ccagatggac ttttttatgc agataaagaa acttttgaag
     1861 caaaaggcgc aaaagtttat atgaactcac cagttgagtc aattgattat gatgcgaaaa
     1921 aagttacagc tattgttgat ggcaaagagc atgtagagtc atatgacaaa ttgattttgg
     1981 caacaggttc tcagccaatt ctgcctccga ttaaaggtgc tgagatggat cctaacagtc
     2041 gtgaatttaa gtcaaccttg gaaaatctgc agtttgttaa attgtatcaa aatgctgctg
     2101 atgttatcga gaaattgcaa gacaagagca aacatatcga acgtgtggca gtagtaggag
     2161 ctggttacat tggggttgag ttagcagaag ccttcaaacg ccttggtaaa gaagtgattc
     2221 tgattgacgt tgtggatact tgcttggcgg gttactatga ccacgacttg tctgaaatga
     2281 tgcgtcaaaa tcttgaagac aatggtgttc aattagcctt tggccaaact gttcaagcta
     2341 ttgaaggtga aaacaaggta gaacgcatag taacggataa agctagctat gatgtagata
     2401 tggtcgtctt ggcagttggc ttccgtccaa acactggtct tggtgctggc aagttggaaa
     2461 cattccgcaa tggtgctttc ttggtggata agaagcaaga aactagcatt aaagatgttt
     2521 atgcaatcgg tgactgtgcg actgtctacg ataactctat caatgataca aactacatcg
     2581 ccttggcatc taacgccctg cgctctggta ttgtagcagc tcataatgct tgcggtcatg
     2641 aattggagtc aaacggtgtt caaggttcta acggtatcga aatctttggt ttgaagatgg
     2701 tttcaactgg tctgactgaa gaaaaagcta aacgctttgg ttacagccca gctgtagttg
     2761 agtttaaaga cactcaaaaa ccaactttcc ttgaaaaggt tgagcatcat gatgttacaa
     2821 ttaagattgt ctatgataag gatacacgtg tagttcttgg agctcagatg gtttctagag
     2881 aagatatgtc tatgggtatt cacatgttct cattggctat tcaggaaaag gttacaattg
     2941 atagattggc cttgctggat cttttcttcc tgccacactt caacaaaccg tacaactaca
     3001 ttactcaagc agctttgaaa gcaaaataat actagagcca ggcatcaaat aaaacgaaag
     3061 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     3121 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//