BBa_K2184038
1
BBa_K2184038
mutant SNP for non taster Umami & Monosodium gluten taste with guidRNA
2016-09-29T11:00:00Z
2016-09-30T10:01:03Z
Engineered mutant SNP for non taster Umami & Monosodium gluten taste- Human genomic.
Engineered guidRNA to this SNP using a tool called Crispr.MIT.Edu
The basic assumption of the project is based on the fact that there is a direct link between particular taste affection and the number of receptors for that taste. For this reason, the ability to control the polymorphism of taste receptors may be useful in many ways to human society, for example, specific desirable diet aid by reducing the ability to feel a specific unhealthy taste, reducing alcohol consumption by changing sensitivity to alcohol, reducing the risk of high blood pressure by eliminating the need for salt taste, changing the sensitivity for bitterness to allow swallowing a pill for the purpose of providing medical care and more.
This molecule was specially designed so that it will able to identify SNPs in one specific flavor-grapefruit juice bitterness. flavors respectively and mediate CRISPR editing of the non-taste alleles only.
בהרחבה
Engineered SNP (Single Nucleotide Polymorphism) in favor of this reaction. This SNP enables identification of a certain taste out of the five known senses of taste, Each of the SNP???s that were selected Refer to people that unables identification of a certain taste out of the five known senses of taste.
Using SNP segments ??? the Taqman reaction makes it possible to identify the presence of a specific allele tested for a specific taste. Whenever a sample DNA is tested a presence of a specific allele is checked by one of the 15 SNP's we receive a fluorescence signal due to the release of the PROBE and vice versa. 67 DNA samples were tested using this method (samples were collected from 67 students and teachers from the school). Each sample was tested to check the presence of alleles to identify different tastes in accordance to the sequence of the SNP.
ThIs molecule WAS specially designed so that it was able to identify SNPs in specific allele flavor respectively and mediate CRISPR editing of the non-taste alleles only.
false
false
_2660_
32581
32581
9
false
Engineered mutant SNP for non taster Umami & Monosodium gluten taste by swich T with C base.
Engineered guidRNA to this SNP using a tool called Crispr.MIT.Edu. guide wAS the same for SNP. the SNP made enough of a difference to create or destroy a PAM site. as Cas9 would cut one kind and not the other (PAM is NGG, so if one SNP had NGG and one had NGC as a SNP,We engineered a guide upstream to this PAM and expect Cas9 to cut the NGG form and not the NGC.)
false
Margalit Mualem
component2485606
1
BBa_K2184035
component2485605
1
BBa_K2184004
annotation2485605
1
BBa_K2184004
range2485605
1
1
20
annotation2485606
1
BBa_K2184035
range2485606
1
29
209
BBa_K2184004
1
BBa_K2184004
Guid RNA for umami&monosodiom glutamat flavor
2016-09-18T11:00:00Z
2016-09-19T08:59:13Z
CTACAACCGTGCCCGTGGCC
Guid RNA for cas9 for umami & monosodiom glutamat flavor-
The basic assumption of the project is based on the fact that there is a direct link between particular taste affection and the number of receptors for that taste. For this reason, the ability to control the polymorphism of taste receptors may be useful in many ways to human society, for example, specific desirable diet aid by reducing the ability to feel a specific unhealthy taste, reducing alcohol consumption by changing sensitivity to alcohol, reducing the risk of high blood pressure by eliminating the need for salt taste, changing the sensitivity for bitterness to allow swallowing a pill for the purpose of providing medical care and more.
false
false
_2660_
32581
32581
9
false
This molecule was specially designed to able to identify umami & monosodiom glutamat SNP and mediates CRISPR editing of the non-taste alleles only
false
Margalit Mualem
BBa_K2184035
1
BBa_K2184035
SNP for non-taster umami & monosodium gluten - TAS1R3 rs307377
2016-09-29T11:00:00Z
2016-09-30T09:13:35Z
Human genomic for non taster umami & monosodium gluten taste
Engineered mutant SNP for non taster grapefruit juice bitterness taste by swich T with C base
Engineered 15 SNPs (Single Nucleotide Polymorphism) in favor of this reaction. Each of the SNP???s that were selected enables identification of a certain taste out of the five known senses of taste, Each of the SNP???s that were selected Refer to people that unables identification of a certain taste out of the five known senses of taste.
Using SNP segments ??? the Taqman reaction makes it possible to identify the presence of a specific allele tested for a specific taste. Whenever a sample DNA is tested a presence of a specific allele is checked by one of the 15 SNP's we receive a fluorescence signal due to the release of the PROBE and vice versa. 67 DNA samples were tested using this method (samples were collected from 67 students and teachers from the school). Each sample was tested to check the presence of alleles to identify different tastes in accordance to the sequence of the SNP.
false
false
_2660_
32581
32581
9
false
Engineered mutant SNP for non taster umami & monosodium gluten taste by swich T with C base
false
Margalit Mualem
BBa_K2184004_sequence
1
ctacaaccgtgcccgtggcc
BBa_K2184038_sequence
1
ctacaaccgtgcccgtggcctactagagcacacgctcctgggtcagcttcggcctagcgcacgccaccaatgccacgctggcctttctctgcttcctgggcactttcctggtgcggagccagccgggccgtcacctttgccatgctggcctacttcatcacctgggtctcctttgtgcccctcctggccaatgtgcaggtggtcctcag
BBa_K2184035_sequence
1
cacacgctcctgggtcagcttcggcctagcgcacgccaccaatgccacgctggcctttctctgcttcctgggcactttcctggtgcggagccagccgggccgtcacctttgccatgctggcctacttcatcacctgggtctcctttgtgcccctcctggccaatgtgcaggtggtcctcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z