BBa_K165002 1 BBa_K165002 Kozak sequence (yeast RBS) 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - Released HQ 2013 The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region. false false _267_ 0 2512 58 In stock false - false John Szymanski BBa_K2185004 1 BBa_K2185004 B.cereus amylase 2016-09-04T11:00:00Z 2016-09-15T01:58:15Z This part comes from the genome of the bacteria B.cereus. This is the coding sequence for a beta amylase enzyme, from B.cereus. false false _2661_ 27970 27970 9 false Codon optimized for S.cerevisiae. false Marissa Sumathipala BBa_K392003 1 BBa_K392003 yeast ADH1 terminator 2010-10-09T11:00:00Z 2015-05-08T01:12:20Z Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak). Engineered 'cyc100 promoter' of <i>Saccharomyces cerevisiae</i> with Kozak sequence attached downstream. false false _528_ 0 6220 9 It's complicated true Assembled by 3A method. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_K792002 1 MFa1-Secre Secretion tag from yeast &#945;-factor mating pheromone (MF&#945;1) 2012-09-23T11:00:00Z 2015-05-08T01:13:23Z Prepro-a-factorHas a Cleavable Signal Sequence [Water et al 1987] This part is the secretion signal peptide for the yeast &#945;-mating factor. This signal peptide directs the secretion of the produced protein, and therefore allows for the exportation of it. This peptides are cleaved once the protein is in the lumen of the ER. false false _1047_ 0 11811 9 Not in stock false None false Manuel Gim??nez annotation2197873 1 secretion tag range2197873 1 1 54 BBa_K2185005 1 BBa_K2185005 B.cereus beta amylase w/ no promoter 2016-09-04T11:00:00Z 2016-09-15T01:58:44Z It is a composite part, coding sequence is sourced from the B.cereus genome. This composite part, designed and submitted by the 2016 Broad Run iGEM Team, contains an gene from the bacterium B.cereus that codes for a beta amylase enzyme. This composite part contains the coding sequence, kozak sequence, a mating factor secretion tag, and a terminator. It does not include a promoter. The amylase enzyme can be expressed by cloning this part into a plasmid with a promoter built in (such as the S.cerevisiae yeast vector pAG36). false false _2661_ 27970 27970 9 false Codon optimized for S.cerevisiae, Kpn and Spe sites removed. false Marissa Sumathipala component2483549 1 BBa_K2185004 component2483548 1 BBa_K792002 component2483546 1 BBa_K165002 component2483550 1 BBa_K392003 annotation2483549 1 BBa_K2185004 range2483549 1 87 1727 annotation2483548 1 BBa_K792002 range2483548 1 27 80 annotation2483550 1 BBa_K392003 range2483550 1 1736 1864 annotation2483546 1 BBa_K165002 range2483546 1 1 18 BBa_K165002_sequence 1 cccgccgccaccatggag BBa_K2185004_sequence 1 atgaagaaccagtttcagtattgttgcattgtcgttctgagtatcgtgatgttatttgtcagcttgttgataccccagacttcttttgcggcagtaaatggcaagggaatgaatcctgactataaggcttatttgatggctccattaaagaaaatacccgaggtgactaactgggaaaccttcgaaaacgaccttaggtgggcaaagcaaaatggtttctacgcaatcactgtagatttctggtggggggacatggagaaaaacggggaccagcaattcgactttagctatgcacagaggtttgcgcaaagtgtccgtaacgcgggtatgaagatgattccgattatatcaacgcaccagtgtggaggcaacgtgggtgatgattgcaatgcccccatccccctatgggtatggaaccagaagaatgatgacagcctttattttaaatccgaaacaggaaccgttaacaaagaaacgttgaacccgttagcgtccgatgttatacaaaaggaatatggagaattgtatacggcctttgctgaagctatgaaaccgtataaagatgtaatcgtcaaaatttatttgtctgggggccctgccggcgagctgcgttatccttcttatacctctgcggacggaagcggttacccatctcgtggtaaattccaggcatatacggaaagcgctaagtcaaaatttaggagttgggttatcaacaagtatgactccctaaacgaggtaaataaagcatggagtacgaaattaaccagctttagtgagattttgcctccgagtgacggagagttgttcctaaaaaagggttataattcctcatacggaaaagactacctggactggtatcaaggtgtgctagagtctcatacaaagctaatcggcgtcctagcgcataacgcgttcgatccctctttccaagtaccgattggagccaaaatagcgggagttcattggcaatataacaatcctgtgattccgcacggcgctgagaggcccgcgggttataatgactattcctacctgctggacgccttcaagtctgcgaaattagatgttactttcacttgtcttgagatgacggataaagggtcctacccggaatattccatgccaagaacattagttcaggaaatcgcgaccctggcgaatcagaaaggagtagtcttgaatggagaaaatgccctaagcattggaaacgagactgagtataatagagtcgccgagatggctttcaactacaatttcgccgggtttactctattaagatatcaggacgttatttacaacaatagtttgatggagaagtttaaaaacctactgggcgtgactccggttatgcaaaccatagtagttaaaaatgttcccacgacactgggcgatacagtttatattaccgggaacagagcggagttaggtcaatgggacaccaagcagtatccaatccaattgtattatgatagtcataataacgactggagaggaaacgtagtattgccagcagagagaaacatcgaatttcgtgcttttatcaaaagcaaagacggtcgtctgaagagctggcaaaccatccaacagagttggaaccctgtcccgatgaaaactacaagccacgttagttggtggtaa BBa_K2185005_sequence 1 cccgccgccaccatggagtactagagagattcccatccattttcaccgctgttttgttcgccgcttcttctgctttggcttactagatgaagaaccagtttcagtattgttgcattgtcgttctgagtatcgtgatgttatttgtcagcttgttgataccccagacttcttttgcggcagtaaatggcaagggaatgaatcctgactataaggcttatttgatggctccattaaagaaaatacccgaggtgactaactgggaaaccttcgaaaacgaccttaggtgggcaaagcaaaatggtttctacgcaatcactgtagatttctggtggggggacatggagaaaaacggggaccagcaattcgactttagctatgcacagaggtttgcgcaaagtgtccgtaacgcgggtatgaagatgattccgattatatcaacgcaccagtgtggaggcaacgtgggtgatgattgcaatgcccccatccccctatgggtatggaaccagaagaatgatgacagcctttattttaaatccgaaacaggaaccgttaacaaagaaacgttgaacccgttagcgtccgatgttatacaaaaggaatatggagaattgtatacggcctttgctgaagctatgaaaccgtataaagatgtaatcgtcaaaatttatttgtctgggggccctgccggcgagctgcgttatccttcttatacctctgcggacggaagcggttacccatctcgtggtaaattccaggcatatacggaaagcgctaagtcaaaatttaggagttgggttatcaacaagtatgactccctaaacgaggtaaataaagcatggagtacgaaattaaccagctttagtgagattttgcctccgagtgacggagagttgttcctaaaaaagggttataattcctcatacggaaaagactacctggactggtatcaaggtgtgctagagtctcatacaaagctaatcggcgtcctagcgcataacgcgttcgatccctctttccaagtaccgattggagccaaaatagcgggagttcattggcaatataacaatcctgtgattccgcacggcgctgagaggcccgcgggttataatgactattcctacctgctggacgccttcaagtctgcgaaattagatgttactttcacttgtcttgagatgacggataaagggtcctacccggaatattccatgccaagaacattagttcaggaaatcgcgaccctggcgaatcagaaaggagtagtcttgaatggagaaaatgccctaagcattggaaacgagactgagtataatagagtcgccgagatggctttcaactacaatttcgccgggtttactctattaagatatcaggacgttatttacaacaatagtttgatggagaagtttaaaaacctactgggcgtgactccggttatgcaaaccatagtagttaaaaatgttcccacgacactgggcgatacagtttatattaccgggaacagagcggagttaggtcaatgggacaccaagcagtatccaatccaattgtattatgatagtcataataacgactggagaggaaacgtagtattgccagcagagagaaacatcgaatttcgtgcttttatcaaaagcaaagacggtcgtctgaagagctggcaaaccatccaacagagttggaaccctgtcccgatgaaaactacaagccacgttagttggtggtaatactagaggcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_K792002_sequence 1 agattcccatccattttcaccgctgttttgttcgccgcttcttctgctttggct BBa_K392003_sequence 1 gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z