BBa_K165002 1 BBa_K165002 Kozak sequence (yeast RBS) 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - Released HQ 2013 The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region. false false _267_ 0 2512 58 In stock false - false John Szymanski BBa_K2185006 1 BBa_K2185006 S.ferax alpha amylase coding sequence 2016-09-04T11:00:00Z 2016-09-15T01:53:49Z It comes from the genome of the fungus S.ferax. This is a coding sequence for the alpha amylase enzyme from the fungus S.ferax. false false _2661_ 27970 27970 9 false Codon optimized for S.cerevisiae, kpn and spe sites removed. false Marissa Sumathipala BBa_I766555 1 BBa_I766555 pCyc (Medium) Promoter 2007-08-15T11:00:00Z 2015-08-31T04:08:10Z Endogenous promoter for Cytochrome c Mid-expression level constitutive promoter in yeast false false _155_ 0 1931 9 Not in stock false none false Nili Sommovilla BBa_K2185007 1 BBa_K2185007 S.ferax alpha amylase w/ cyc promoter 2016-09-04T11:00:00Z 2016-09-15T01:55:03Z The coding sequence of this part comes from the fungus S.ferax This composite part, designed and submitted by the 2016 Broad Run iGEM Team, contains an gene from the fungus S.ferax that codes for an alpha amylase enzyme. This composite part contains a constitutive promoter, coding sequence, kozak sequence, a mating factor secretion tag, and a terminator. The amylase enzyme can be expressed by cloning this part into a plasmid without a promoter built in (such as the S.cerevisiae vector pRS426). false false _2661_ 27970 27970 9 false Codon optimized for S.cerevisiae, EcoR1 and Spe1 sites removed. false Broad Run iGEM Team component2483531 1 BBa_I766555 component2483532 1 BBa_K165002 component2483535 1 BBa_K2185006 component2483534 1 BBa_K792002 component2483536 1 BBa_K392003 annotation2483536 1 BBa_K392003 range2483536 1 1752 1880 annotation2483531 1 BBa_I766555 range2483531 1 1 244 annotation2483532 1 BBa_K165002 range2483532 1 253 270 annotation2483534 1 BBa_K792002 range2483534 1 279 332 annotation2483535 1 BBa_K2185006 range2483535 1 339 1743 BBa_K392003 1 BBa_K392003 yeast ADH1 terminator 2010-10-09T11:00:00Z 2015-05-08T01:12:20Z Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak). Engineered 'cyc100 promoter' of <i>Saccharomyces cerevisiae</i> with Kozak sequence attached downstream. false false _528_ 0 6220 9 It's complicated true Assembled by 3A method. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_K792002 1 MFa1-Secre Secretion tag from yeast &#945;-factor mating pheromone (MF&#945;1) 2012-09-23T11:00:00Z 2015-05-08T01:13:23Z Prepro-a-factorHas a Cleavable Signal Sequence [Water et al 1987] This part is the secretion signal peptide for the yeast &#945;-mating factor. This signal peptide directs the secretion of the produced protein, and therefore allows for the exportation of it. This peptides are cleaved once the protein is in the lumen of the ER. false false _1047_ 0 11811 9 Not in stock false None false Manuel Gim??nez annotation2197873 1 secretion tag range2197873 1 1 54 BBa_K165002_sequence 1 cccgccgccaccatggag BBa_K2185007_sequence 1 cagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaatatactagagcccgccgccaccatggagtactagagagattcccatccattttcaccgctgttttgttcgccgcttcttctgctttggcttactagatggtgcgtctccttccttcgctcgtcgccgccacgctcgcgcttgtcgccgccgaggtgcctgtcaacgtcatgctcccgctcgacacggtcgttttggactcgaaggcgtgcagcagcacccggctcaagaatgcgacggcgctgagcctccaatttgagaagctcaaggcgagcggcgccacaggtgtcatggccgactgctggtggggcctcgtcgaaggcgcgggcccgcgccagtacgactttgccgcgtacgccgaccttgcgcggcttgccaaggcgtccaacctcacgattcagatggtcatgtccttccaccagtgcggtggcaacgtcggcgatgagtgcgacatcccgatcccgcgccactggttcaccaaggacgacgtgtggtacacaacgcaggccggcttgatcacgaaggagtacatctcgctctgggccgacgcaacgccgctcgacaagtttggccgcacgccgctccagatgtacagcgagttcctcgcggccttcaagacgcacgtcgtcgacgcgtatcctggtgtcgtctcggaggtccagatcggcggtggccccgcgggcgagctccgatacccctcgtaccagctccaggagaaccggtggagctactgcggcgtcggtgagtttacgagctacgacacgtacgcgaacgcaagcatcgtggcgcacgccgcgtcgacgggccacgcgctttgggctacgcgcccgggtccgaacaacgccggcacgtttaactgccttccgagcgaaaacggccagtgcccgttctttgcgaatggtgccgacaactttgcgagtccgtacggccagttttacctcgactggtacagcgggtcgctgctccagcacggacgcgacctcacgaaactcgggcgcgacgtcttccccgcgccgtttgagctctcggtcaaggtctctggcatccactggtggtacgactcgccgcaccacggtgctgagctcacggccggataccaaaacaccaataacaagaacgcgtactacgacattgcttcgatgctcaaggagcacgacgtgcgcttctgctttacgtgcatggagatgaacgacaactacgacgacaacgacaagtgccgatcgcgccccgggaagctcgtgggccaggcccgcgacgccgtcacggcgctcggccttagtttgaagcacagctttgcgggcgagaacgcgcttccgattggcggcaacgaccaaatcacgtcgatcgcgggccacatcgctggtgccgcgtcgtttacgtttttgcggctcaccgacacgtttgactttgactacttgacgcgcctcgtgcagcgcttgaagacggtctaatgctctcctatatccgatcctgtatataaaaagtggtgttgtatactcatgatactagaggcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_I766555_sequence 1 cagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaata BBa_K2185006_sequence 1 atggtgcgtctccttccttcgctcgtcgccgccacgctcgcgcttgtcgccgccgaggtgcctgtcaacgtcatgctcccgctcgacacggtcgttttggactcgaaggcgtgcagcagcacccggctcaagaatgcgacggcgctgagcctccaatttgagaagctcaaggcgagcggcgccacaggtgtcatggccgactgctggtggggcctcgtcgaaggcgcgggcccgcgccagtacgactttgccgcgtacgccgaccttgcgcggcttgccaaggcgtccaacctcacgattcagatggtcatgtccttccaccagtgcggtggcaacgtcggcgatgagtgcgacatcccgatcccgcgccactggttcaccaaggacgacgtgtggtacacaacgcaggccggcttgatcacgaaggagtacatctcgctctgggccgacgcaacgccgctcgacaagtttggccgcacgccgctccagatgtacagcgagttcctcgcggccttcaagacgcacgtcgtcgacgcgtatcctggtgtcgtctcggaggtccagatcggcggtggccccgcgggcgagctccgatacccctcgtaccagctccaggagaaccggtggagctactgcggcgtcggtgagtttacgagctacgacacgtacgcgaacgcaagcatcgtggcgcacgccgcgtcgacgggccacgcgctttgggctacgcgcccgggtccgaacaacgccggcacgtttaactgccttccgagcgaaaacggccagtgcccgttctttgcgaatggtgccgacaactttgcgagtccgtacggccagttttacctcgactggtacagcgggtcgctgctccagcacggacgcgacctcacgaaactcgggcgcgacgtcttccccgcgccgtttgagctctcggtcaaggtctctggcatccactggtggtacgactcgccgcaccacggtgctgagctcacggccggataccaaaacaccaataacaagaacgcgtactacgacattgcttcgatgctcaaggagcacgacgtgcgcttctgctttacgtgcatggagatgaacgacaactacgacgacaacgacaagtgccgatcgcgccccgggaagctcgtgggccaggcccgcgacgccgtcacggcgctcggccttagtttgaagcacagctttgcgggcgagaacgcgcttccgattggcggcaacgaccaaatcacgtcgatcgcgggccacatcgctggtgccgcgtcgtttacgtttttgcggctcaccgacacgtttgactttgactacttgacgcgcctcgtgcagcgcttgaagacggtctaatgctctcctatatccgatcctgtatataaaaagtggtgttgtatactcatga BBa_K792002_sequence 1 agattcccatccattttcaccgctgttttgttcgccgcttcttctgctttggct BBa_K392003_sequence 1 gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z