LOCUS       BBa_K2187000            1097 bp    DNA     linear       09-Jan-2025
DEFINITION  Lipase gene
ACCESSION   BBa_K2187000
VERSION     BBa_K2187000.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     CDS             62..937
                     /label=BBa_K2187002
     RBS             946..960
                     /label=RBS-1\Strong
     RBS             953..957
                     /label=RBS
     misc_feature    946..960
                     /label=BBa_B0030
     RBS             946..960
                     /label=BBa_B0030
     stem_loop       980..1023
                     /label=stem_loop
     misc_feature    969..1048
                     /label=BBa_B0010
     terminator      969..1048
                     /label=BBa_B0010
     stem_loop       1064..1083
                     /label=T7 TE
     misc_feature    1090..1090
                     /label=stop
     polyA_site      1084..1097
                     /label=polya
     misc_feature    1057..1097
                     /label=BBa_B0012
     terminator      1057..1097
                     /label=BBa_B0012
     terminator      969..1097
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gatgaatttc ccgcgtgcaa gtcgcttgat gcaggctgct gtcttgggag gacttatggc
      121 cgtctccgca gctgccacag cgcagacgaa cccatatgca cgcggcccga acccaaccgc
      181 cgcatccctg gaagcttccg ctggaccatt cacagtacgc tcatttaccg ttagtcgtcc
      241 gagtggctac ggtgctggaa ccgtatacta cccaaccaat gccgggggaa cagtgggcgc
      301 cattgcaatc gtccctgggt ataccgcgcg tcaaagttct attaagtggt ggggaccacg
      361 cttggcgtcc catggcttcg tggtaatcac gattgatacg aacagcacct tggatcagcc
      421 cagtagccgt agtagccaac agatggcagc cttgcgccaa gtggcatcct tgaatggtac
      481 ttctagctca ccaatctatg gcaaagtgga cacagctcgt atgggagtca tgggttggtc
      541 gatgggaggt ggtggatcgt tgatttctgc ggccaataat ccaagcttga aggcggcggc
      601 accccaggca ccctgggact caagcacgaa cttctcgagt gtgaccgttc caacccttat
      661 tttcgcatgt gaaaacgact cgattgcacc cgtgaactcg agtgccttac ccatttacga
      721 ctctatgtcc cgtaacgcga aacagtttct tgagattaac ggaggatcgc acagttgtgc
      781 caattcgggt aattccaatc aagcgcttat cggtaaaaaa ggcgtcgcct ggatgaagcg
      841 cttcatggac aatgatactc gttactccac cttcgcctgt gaaaacccta actcaactcg
      901 tgtttcagac ttccgtactg cgaactgtag ctaataatac tagagattaa agaggagaaa
      961 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1021 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1081 gcctttctgc gtttata
//