BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2000
1
-35
range2000
1
20
25
annotation2002
1
-10
range2002
1
43
48
annotation2001
1
lac O1
range2001
1
26
42
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation1999
1
lac O1
range1999
1
3
19
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K2187002
1
BBa_K2187002
Lipase gene
2016-10-13T11:00:00Z
2016-10-14T04:18:26Z
We used the sequence of the genomic DNA:GAP38373.1 from GenBank.
The Lipase gene comes from the Ideonella sakaiensis bacteria. Its purpose is to create and enzyme called MHETase that breaks down MHET into ethylene glycol and terephthelic acid.
false
false
_2668_
0
2758
2758
9
Not in stock
false
There were no problesm
false
Lisa Scheifele
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K2187000
1
BBa_K2187000
Lipase gene
2016-10-13T11:00:00Z
2016-10-14T04:23:01Z
We used the sequence of the genomic DNA:GAP38373.1 from GenBank.
The Lipase gene comes from the Ideonella sakaiensis bacteria. Its purpose is to create and enzyme called MHETase that breaks down MHET into ethylene glycol and terephthelic acid.
false
false
_2668_
0
2758
2758
9
Not in stock
false
We added a lacl regulated promoter, BBa_R0011, so that we could induce it to very high levels of expression. We also added a prefix and suffix, a BBa_B0030 RBS, and a BBa_B0015 terminator.
false
Ella Coleman
component2509860
1
BBa_B0030
component2509868
1
BBa_B0015
component2509858
1
BBa_K2187002
component2509853
1
BBa_R0011
annotation2509858
1
BBa_K2187002
range2509858
1
62
937
annotation2509853
1
BBa_R0011
range2509853
1
1
54
annotation2509868
1
BBa_B0015
range2509868
1
969
1097
annotation2509860
1
BBa_B0030
range2509860
1
946
960
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K2187000_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagatgaatttcccgcgtgcaagtcgcttgatgcaggctgctgtcttgggaggacttatggccgtctccgcagctgccacagcgcagacgaacccatatgcacgcggcccgaacccaaccgccgcatccctggaagcttccgctggaccattcacagtacgctcatttaccgttagtcgtccgagtggctacggtgctggaaccgtatactacccaaccaatgccgggggaacagtgggcgccattgcaatcgtccctgggtataccgcgcgtcaaagttctattaagtggtggggaccacgcttggcgtcccatggcttcgtggtaatcacgattgatacgaacagcaccttggatcagcccagtagccgtagtagccaacagatggcagccttgcgccaagtggcatccttgaatggtacttctagctcaccaatctatggcaaagtggacacagctcgtatgggagtcatgggttggtcgatgggaggtggtggatcgttgatttctgcggccaataatccaagcttgaaggcggcggcaccccaggcaccctgggactcaagcacgaacttctcgagtgtgaccgttccaacccttattttcgcatgtgaaaacgactcgattgcacccgtgaactcgagtgccttacccatttacgactctatgtcccgtaacgcgaaacagtttcttgagattaacggaggatcgcacagttgtgccaattcgggtaattccaatcaagcgcttatcggtaaaaaaggcgtcgcctggatgaagcgcttcatggacaatgatactcgttactccaccttcgcctgtgaaaaccctaactcaactcgtgtttcagacttccgtactgcgaactgtagctaataatactagagattaaagaggagaaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K2187002_sequence
1
atgaatttcccgcgtgcaagtcgcttgatgcaggctgctgtcttgggaggacttatggccgtctccgcagctgccacagcgcagacgaacccatatgcacgcggcccgaacccaaccgccgcatccctggaagcttccgctggaccattcacagtacgctcatttaccgttagtcgtccgagtggctacggtgctggaaccgtatactacccaaccaatgccgggggaacagtgggcgccattgcaatcgtccctgggtataccgcgcgtcaaagttctattaagtggtggggaccacgcttggcgtcccatggcttcgtggtaatcacgattgatacgaacagcaccttggatcagcccagtagccgtagtagccaacagatggcagccttgcgccaagtggcatccttgaatggtacttctagctcaccaatctatggcaaagtggacacagctcgtatgggagtcatgggttggtcgatgggaggtggtggatcgttgatttctgcggccaataatccaagcttgaaggcggcggcaccccaggcaccctgggactcaagcacgaacttctcgagtgtgaccgttccaacccttattttcgcatgtgaaaacgactcgattgcacccgtgaactcgagtgccttacccatttacgactctatgtcccgtaacgcgaaacagtttcttgagattaacggaggatcgcacagttgtgccaattcgggtaattccaatcaagcgcttatcggtaaaaaaggcgtcgcctggatgaagcgcttcatggacaatgatactcgttactccaccttcgcctgtgaaaaccctaactcaactcgtgtttcagacttccgtactgcgaactgtagctaataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z