BBa_K2187001
1
BBa_K2187001
Chlorogenate Esterase gene
2016-10-13T11:00:00Z
2016-10-14T04:44:45Z
We used the genomic sequence of DNA: GAP38911.1 from GenBank.
The chlorogenate esterase gene comes from the Ideonella sakaiensis bacteria. Its purpose is to create an enzyme called PETase which breaks down PET into MHET.
false
false
_2668_
2758
2758
9
false
We added a lacl regulated promoter, BBa_R0011, to allow us to turn up enzyme production. In addition, we added a prefix and suffix, a BBa_B0030 RBS, and a BBa_K864600. We also had to change two internal Pst1 sites.
false
Ella Coleman
component2509927
1
BBa_K2187003
component2509932
1
BBa_K864600
component2509929
1
BBa_B0030
component2509922
1
BBa_R0011
annotation2509922
1
BBa_R0011
range2509922
1
1
54
annotation2509927
1
BBa_K2187003
range2509927
1
64
2040
annotation2509932
1
BBa_K864600
range2509932
1
2072
2123
annotation2509929
1
BBa_B0030
range2509929
1
2049
2063
BBa_K2187003
1
BBa_K2187003
Chlorogenate Esterase gene
2016-10-13T11:00:00Z
2016-10-14T04:42:18Z
We used the genomic sequence of DNA: GAP38911.1 from GenBank.
The chlorogenate esterase gene comes from the Ideonella sakaiensis bacteria. Its purpose is to create an enzyme called PETase which breaks down PET into MHET.
false
false
_2668_
2758
2758
9
false
We had to change two internal PstI sites
false
Lisa Scheifele
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2001
1
lac O1
range2001
1
26
42
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation1999
1
lac O1
range1999
1
3
19
annotation2000
1
-35
range2000
1
20
25
annotation2002
1
-10
range2002
1
43
48
BBa_K864600
1
BBa_K864600
T0 (21 imm) transcriptional terminator
2012-09-24T11:00:00Z
2015-05-08T01:13:37Z
Gene synthesis
Released HQ 2013
The T0 transcriptional terminator terminates transcription with 80-90% efficiency.
false
false
_1124_
0
9827
9
In stock
false
-
false
Erik Lundin
annotation2197273
1
T0 terminator
range2197273
1
1
52
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K2187003_sequence
1
actatgaattcgcggccgcttctagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagctagagattaaagaggagaaatactagatgcaaacgacggtcaccactatgcttttagcttccgtcgcgttggccgcgtgtgctgggggcggatccacaccattgccattacctcagcaacagccgccacagcaggaaccgccgcccccgcccgtaccactggcatcccgtgcagcgtgtgaggccttgaaggatgggaacggggatatggtttggccaaatgctgcaaccgtggtcgaagtggccgcttggcgcgacgccgcacccgcaaccgcatcggcagcagctttgcccgagcattgtgaagtaagtggggccattgcaaaacgcacaggaattgatggatatccttacgagattaagttccgtttacgcatgccggctgaatggaacggccgctttttcatggaaggtgggtccggaactaacgggtccttatcagcggcgacgggtagtattggtggagggcagatcgccagcgccctgtcacgcaatttcgcaacaatcgcaaccgacggcggacacgacaacgcggtcaatgataatcccgacgcattagggactgtcgcattcgggctggaccctcaggcccgccttgatatgggttacaattcgtacgaccaagtcactcaagcgggcaaagcagcagttgcccgtttttacgggcgcgctgctgataagtcgtattttatcgggtgctcggaagggggccgcgagggcatgatgctttcccaacgtttcccctcgcactatgacggaattgttgctggcgcacccgggtaccagttacctaaagctgggatcagcggagcctggactactcaaagtcttgccccggctgccgtggggttagatgcacagggggtccctcttatcaataagtcattctctgacgcagaccttcacttgttgagtcaggccattcttgggacatgtgacgctttggacggtctggctgacggaatcgtggacaattaccgcgcttgccaagctgccttcgatccggccacggccgctaaccctgctaatgggcaggcgcttcaatgcgtcggtgcaaagaccgccgactgtctttccccagtccaggtgaccgctatcaaacgcgctatggcgggccctgtcaactctgcgggcactccactttacaatcgctgggcctgggacgctggcatgtctggtctgtctgggacaacatacaatcagggatggcgcagttggtggcttgggagtttcaattcgtcagctaataatgcacaacgtgtaagtggtttcagtgcccgtagctggctggtagattttgcaactccgccggagcccatgcccatgactcaggtggctgcccgtatgatgaaatttgattttgacatcgatccgttaaaaatctgggcgacatctgggcagttcacacaatcttcgatggattggcatggggctacttctaccgaccttgctgcgtttcgtgaccgtggcgggaaaatgatcttataccacgggatgtcagatgctgcgttctcggctcttgacacagctgactattatgagcgccttggtgcggcaatgcccggagctgctgggttcgcccgtctgttcttagtgccgggtatgaaccattgcagtggcggtcccgggacagaccgcttcgatatgttgactccactggtggcctgggtggaacgtggagaggcgcccgatcaaatttcggcttggtccggcacaccaggatacttcggggtggcggcccgtacacgtcctttatgtccgtatccccaaatcgcgcgttacaagggttcgggggatattaacacagaagctaactttgcctgtgcagcccccccgtaataattgttcagaacgctcggtcttgcacaccgggcgttttttctttgtgagtcca
BBa_K864600_sequence
1
ttgttcagaacgctcggtcttgcacaccgggcgttttttctttgtgagtcca
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K2187001_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagactatgaattcgcggccgcttctagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagctagagattaaagaggagaaatactagatgcaaacgacggtcaccactatgcttttagcttccgtcgcgttggccgcgtgtgctgggggcggatccacaccattgccattacctcagcaacagccgccacagcaggaaccgccgcccccgcccgtaccactggcatcccgtgcagcgtgtgaggccttgaaggatgggaacggggatatggtttggccaaatgctgcaaccgtggtcgaagtggccgcttggcgcgacgccgcacccgcaaccgcatcggcagcagctttgcccgagcattgtgaagtaagtggggccattgcaaaacgcacaggaattgatggatatccttacgagattaagttccgtttacgcatgccggctgaatggaacggccgctttttcatggaaggtgggtccggaactaacgggtccttatcagcggcgacgggtagtattggtggagggcagatcgccagcgccctgtcacgcaatttcgcaacaatcgcaaccgacggcggacacgacaacgcggtcaatgataatcccgacgcattagggactgtcgcattcgggctggaccctcaggcccgccttgatatgggttacaattcgtacgaccaagtcactcaagcgggcaaagcagcagttgcccgtttttacgggcgcgctgctgataagtcgtattttatcgggtgctcggaagggggccgcgagggcatgatgctttcccaacgtttcccctcgcactatgacggaattgttgctggcgcacccgggtaccagttacctaaagctgggatcagcggagcctggactactcaaagtcttgccccggctgccgtggggttagatgcacagggggtccctcttatcaataagtcattctctgacgcagaccttcacttgttgagtcaggccattcttgggacatgtgacgctttggacggtctggctgacggaatcgtggacaattaccgcgcttgccaagctgccttcgatccggccacggccgctaaccctgctaatgggcaggcgcttcaatgcgtcggtgcaaagaccgccgactgtctttccccagtccaggtgaccgctatcaaacgcgctatggcgggccctgtcaactctgcgggcactccactttacaatcgctgggcctgggacgctggcatgtctggtctgtctgggacaacatacaatcagggatggcgcagttggtggcttgggagtttcaattcgtcagctaataatgcacaacgtgtaagtggtttcagtgcccgtagctggctggtagattttgcaactccgccggagcccatgcccatgactcaggtggctgcccgtatgatgaaatttgattttgacatcgatccgttaaaaatctgggcgacatctgggcagttcacacaatcttcgatggattggcatggggctacttctaccgaccttgctgcgtttcgtgaccgtggcgggaaaatgatcttataccacgggatgtcagatgctgcgttctcggctcttgacacagctgactattatgagcgccttggtgcggcaatgcccggagctgctgggttcgcccgtctgttcttagtgccgggtatgaaccattgcagtggcggtcccgggacagaccgcttcgatatgttgactccactggtggcctgggtggaacgtggagaggcgcccgatcaaatttcggcttggtccggcacaccaggatacttcggggtggcggcccgtacacgtcctttatgtccgtatccccaaatcgcgcgttacaagggttcgggggatattaacacagaagctaactttgcctgtgcagcccccccgtaataattgttcagaacgctcggtcttgcacaccgggcgttttttctttgtgagtccatactagagattaaagaggagaaatactagagttgttcagaacgctcggtcttgcacaccgggcgttttttctttgtgagtcca
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z