BBa_K220002
1
BBa_K220002
Glycine Sarcosine Methyl Transferase Gene
2009-10-17T11:00:00Z
2015-05-08T01:11:30Z
Codon optomization and synthesis of its coding region from ''Ectothiorhodospira Halochloris''
GSMT is a plasmid gene (854 bp) that codes for the enzyme that catalyze the first reaction in the salt tolerant pathway that we are interested. GSMT stands for Glycine Sarcosine N-Methyl Transferase. It catalyzes the conversion from glycine to sarcosine, using SAM as a methyl donor. In our project, we have this GSMT synthesized using MR. Gene. Naturally, this gene can be found in Galdieria sulphuraria, an extremephile that establishes high resistance to osmotic stress as well as acidic, thermal stresses and toxic metals. We PCR amplified gene GSMT with iGEM designated cutting sites and ribosome binding sites and biobricked it with pSB1A2.
'''Reference:''' A Nyyss??l??, T Reinikainen, M Leisola
''Characterization of Glycine Sarcosine N-Methyltransferase and Sarcosine Dimethylglycine N-Methyltransferase''
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92834/?tool=pubmed
false
true
_323_
0
4504
9
It's complicated
false
Codon optimization and synthesis of its coding region from ''Ectothiorhodospira Halochloris''
false
Sarah Sandock
BBa_K220002_sequence
1
gagctcctgcagagtactagttcagtcttttttggccacatgaatatagaagtccggatcatcttggtgataggtttcttggaaatcgccataggtggtgatgtcttggaaaccaacttcatgcagcagacgacgaacataatctttgcgcagagggaacatgttcaggtgatagacactttggtccggaaactcataacggaagcgggccaggccatcgtcgacatactccggctctgctttaacgttgtcgccacaataataatatgtgtgtttgcttttgaacccttcatccaggatcaggtcatagttacgctgatccagaatcaggatcccatcatgtttcagcacggaatagaactcggccagtgttttacggcgatcttcttcgctgaacaggtgtgtaaaactgttacccagacaaatcacggcatcaaagcgctcttggatatgacgagtaacctgacgccaatcgctatggacggtacgcagaatctggtctttacgggtggcattttcaaaggctttcaccagcatctcaacggaaccatcaacgctagcaacatcgaacccggcttcaatcaggcgaatgctgtggaaaccagtaccagtagcggcatccagcacacgtttggcaccgtgttctttcagggttttgataaaaaactcgccttctgaacttgaacgagcatgccagttgatcagctcatcccatttctccacgaagccctcaatatactcattctgatagtggtcggaatcacgaacttcgatcggattttcgccatagtcctggacctgtttctctttaatcgccattaatatatcctccttctagagatgaattcggtacc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z