BBa_K220002 1 BBa_K220002 Glycine Sarcosine Methyl Transferase Gene 2009-10-17T11:00:00Z 2015-05-08T01:11:30Z Codon optomization and synthesis of its coding region from ''Ectothiorhodospira Halochloris'' GSMT is a plasmid gene (854 bp) that codes for the enzyme that catalyze the first reaction in the salt tolerant pathway that we are interested. GSMT stands for Glycine Sarcosine N-Methyl Transferase. It catalyzes the conversion from glycine to sarcosine, using SAM as a methyl donor. In our project, we have this GSMT synthesized using MR. Gene. Naturally, this gene can be found in Galdieria sulphuraria, an extremephile that establishes high resistance to osmotic stress as well as acidic, thermal stresses and toxic metals. We PCR amplified gene GSMT with iGEM designated cutting sites and ribosome binding sites and biobricked it with pSB1A2. '''Reference:''' A Nyyss??l??, T Reinikainen, M Leisola ''Characterization of Glycine Sarcosine N-Methyltransferase and Sarcosine Dimethylglycine N-Methyltransferase'' http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92834/?tool=pubmed false true _323_ 0 4504 9 It's complicated false Codon optimization and synthesis of its coding region from ''Ectothiorhodospira Halochloris'' false Sarah Sandock BBa_K220002_sequence 1 gagctcctgcagagtactagttcagtcttttttggccacatgaatatagaagtccggatcatcttggtgataggtttcttggaaatcgccataggtggtgatgtcttggaaaccaacttcatgcagcagacgacgaacataatctttgcgcagagggaacatgttcaggtgatagacactttggtccggaaactcataacggaagcgggccaggccatcgtcgacatactccggctctgctttaacgttgtcgccacaataataatatgtgtgtttgcttttgaacccttcatccaggatcaggtcatagttacgctgatccagaatcaggatcccatcatgtttcagcacggaatagaactcggccagtgttttacggcgatcttcttcgctgaacaggtgtgtaaaactgttacccagacaaatcacggcatcaaagcgctcttggatatgacgagtaacctgacgccaatcgctatggacggtacgcagaatctggtctttacgggtggcattttcaaaggctttcaccagcatctcaacggaaccatcaacgctagcaacatcgaacccggcttcaatcaggcgaatgctgtggaaaccagtaccagtagcggcatccagcacacgtttggcaccgtgttctttcagggttttgataaaaaactcgccttctgaacttgaacgagcatgccagttgatcagctcatcccatttctccacgaagccctcaatatactcattctgatagtggtcggaatcacgaacttcgatcggattttcgccatagtcctggacctgtttctctttaatcgccattaatatatcctccttctagagatgaattcggtacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z