BBa_K220005
1
BBa_K220005
Methionine Kinase
2009-10-17T11:00:00Z
2015-05-08T01:11:31Z
Gene isolated from Streptomyces Spectabilis
metK is a plasmid gene (1200 bp) that codes for the enzyme that catalyzes the reaction which produces a methyl donor which is used in the conversion of Glycine to Glycine betaine. The enzyme for this reaction is S-adenosyl-methionine (SAM) synthetase. S-adenosyl-methionine produced from Methionine and one ATP molecule in the presence of SAM synthetase. Three SAM molecules are used in the methylation of Glycine to Glycine betaine (trimethyl glycine). In our project Glycine betaine (an osmoprotectant) was synthesized in E. coli to increase salt tolerance. It was hypothesized that an over expression of metK would lead to an increased production of Glycine betaine.
'''References:''' Y. Wei and E.B. Newman, ''Studies on role of metk gene product of Escherichia coli K 12, Molecular Microbiology (2002)''
false
false
_323_
0
4504
9
No part sequence
false
Length: 38
Fwd: 5'- CGA TCT AGA AGG AGG ATA TAT TAA TGT CCC GCC GTC TC -3'
Length: 33
Rev: 5'- TCA CTG CAG ATC ACT AGT TCA GTG AAC AAG CGG-3'
false
Sarah Sandock
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z