LOCUS       BBa_K220006             1527 bp    DNA     linear       22-Nov-2024
DEFINITION  Salt Stress Activated Promoter Pro U and RBS+RFP+Term
ACCESSION   BBa_K220006
VERSION     BBa_K220006.1  
FEATURES             Location/Qualifiers
     promoter        1..658
                     /label=BBa_K220004
     misc_feature    671..674
                     /label=conserved
     RBS             667..678
                     /label=BBa_B0034
     CDS             685..1359
                     /label=mrfp1
     misc_feature    1366..1390
                     /label=Help:Barcodes
     CDS             685..1365
                     /label=BBa_E1010
     stem_loop       1410..1453
                     /label=stem_loop
     misc_feature    1399..1478
                     /label=BBa_B0010
     terminator      1399..1478
                     /label=BBa_B0010
     stem_loop       1494..1513
                     /label=T7 TE
     misc_feature    1520..1520
                     /label=stop
     polyA_site      1514..1527
                     /label=polya
     misc_feature    1487..1527
                     /label=BBa_B0012
     terminator      1487..1527
                     /label=BBa_B0012
     misc_feature    667..1527
                     /label=BBa_I13507
ORIGIN
        1 aatatcacta cccgcagcag ggaaataatt cccgccaaat agctttttat cacgcaaata
       61 atttgtggtg atctacactg atactctgtt gcattattcg cctgaaacca caatattcag
      121 gcgttttttc gctatctttg acaaaaaata tcaactttct cgatttgctc tcagccctta
      181 tatcacggga aattccggcg atttgctcgc atcaatattc atgccacatt tgccatcagg
      241 ggttgcctca gattctcagt atgttagggt agaaaaaagt gactatttcc attgggtaat
      301 atatcgacat agacaaataa aggaatcttt ctattgcatg gcaattaaat tagaaattaa
      361 aaatctttat aaaatatttg gcgagcatcc acagcgagcg ttcaaatata tcgaacaagg
      421 actttcaaaa gaacaaattc tggaaaaaac tgggctatcg cttggcgtaa aagacgccag
      481 tctggccatt gaagaaggcg agatatttgt catcatggga ttatccggct cgggtaaatc
      541 cacaatggta cgccttctca atcgcctgat tgaacccacc cgcgggcaag tgctgattga
      601 tggtgtggat attgccaaaa tatccgacgc cgaactccgt gaggtgcgca gaaaaaagta
      661 ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg
      721 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa
      781 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt
      841 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac
      901 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa
      961 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc
     1021 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac
     1081 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg
     1141 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac
     1201 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct
     1261 tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa
     1321 cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag
     1381 tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1441 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1501 cttcgggtgg gcctttctgc gtttata
//