LOCUS       BBa_K223004              690 bp    DNA     linear       16-Feb-2025
DEFINITION  RBS + SoxR + Terminator + SoxS promoter -
ACCESSION   BBa_K223004
VERSION     BBa_K223004.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..483
                     /label=BBa_K223000
     stem_loop       503..546
                     /label=stem_loop
     misc_feature    492..571
                     /label=BBa_B0010
     terminator      492..571
                     /label=BBa_B0010
     stem_loop       587..606
                     /label=T7 TE
     misc_feature    613..613
                     /label=stop
     polyA_site      607..620
                     /label=polya
     misc_feature    580..620
                     /label=BBa_B0012
     terminator      580..620
                     /label=BBa_B0012
     promoter        629..690
                     /label=BBa_K223001
ORIGIN
        1 aaagaggaga aatactagat ggaaaagaaa ttaccccgca ttaaagcgct gctaaccccc
       61 ggcgaagtgg cgaaacgcag cggtgtggcg gtatcggcgc tgcatttcta tgaaagtaaa
      121 gggttgatta ccagtatccg taacagcggc aatcagcggc gatataaacg tgatgtgttg
      181 cgatatgttg caattatcaa aattgctcag cgtattggca ttccgctggc gaccattggt
      241 gaagcgtttg gcgtgttgcc cgaagggcat acgttaagtg cgaaagagtg gaaacagctt
      301 tcgtcccaat ggcgagaaga gttggatcgg cgcattcata ccttagtggc gctgcgtgac
      361 gaactggacg gatgtattgg ttgtggctgc ctttcgcgca gtgattgccc gttgcgtaac
      421 ccgggcgacc gcttaggaga agaaggtacc ggcgcacgct tgctggaaga tgaacaaaac
      481 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      541 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      601 tgggcctttc tgcgtttata tactagagaa tcgctttacc tcaagttaac ttgaggaatt
      661 atactcccca acagatgaat taacgaactg
//