LOCUS       BBa_K223005             1573 bp    DNA     linear       22-Dec-2024
DEFINITION  RBS + SoxR +TT + SoxS + RBS + GFP + TT- checks our sequences
ACCESSION   BBa_K223005
VERSION     BBa_K223005.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..483
                     /label=BBa_K223000
     stem_loop       503..546
                     /label=stem_loop
     misc_feature    492..571
                     /label=BBa_B0010
     terminator      492..571
                     /label=BBa_B0010
     stem_loop       587..606
                     /label=T7 TE
     misc_feature    613..613
                     /label=stop
     polyA_site      607..620
                     /label=polya
     misc_feature    580..620
                     /label=BBa_B0012
     terminator      580..620
                     /label=BBa_B0012
     promoter        629..690
                     /label=BBa_K223001
     misc_feature    703..706
                     /label=conserved
     RBS             699..710
                     /label=BBa_B0034
     CDS             717..1436
                     /label=GFP protein
     CDS             717..1436
                     /label=BBa_E0040
     stem_loop       1456..1499
                     /label=stem_loop
     misc_feature    1445..1524
                     /label=BBa_B0010
     terminator      1445..1524
                     /label=BBa_B0010
     stem_loop       1540..1559
                     /label=T7 TE
     misc_feature    1566..1566
                     /label=stop
     polyA_site      1560..1573
                     /label=polya
     misc_feature    1533..1573
                     /label=BBa_B0012
     terminator      1533..1573
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggaaaagaaa ttaccccgca ttaaagcgct gctaaccccc
       61 ggcgaagtgg cgaaacgcag cggtgtggcg gtatcggcgc tgcatttcta tgaaagtaaa
      121 gggttgatta ccagtatccg taacagcggc aatcagcggc gatataaacg tgatgtgttg
      181 cgatatgttg caattatcaa aattgctcag cgtattggca ttccgctggc gaccattggt
      241 gaagcgtttg gcgtgttgcc cgaagggcat acgttaagtg cgaaagagtg gaaacagctt
      301 tcgtcccaat ggcgagaaga gttggatcgg cgcattcata ccttagtggc gctgcgtgac
      361 gaactggacg gatgtattgg ttgtggctgc ctttcgcgca gtgattgccc gttgcgtaac
      421 ccgggcgacc gcttaggaga agaaggtacc ggcgcacgct tgctggaaga tgaacaaaac
      481 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      541 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      601 tgggcctttc tgcgtttata tactagagaa tcgctttacc tcaagttaac ttgaggaatt
      661 atactcccca acagatgaat taacgaactg tactagagaa agaggagaaa tactagatgc
      721 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      781 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      841 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
      901 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
      961 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     1021 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
     1081 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     1141 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     1201 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     1261 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     1321 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     1381 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact
     1441 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1501 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1561 ttctgcgttt ata
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