BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K223009 1 BBa_K223009 RBS + RALDH II + TT 2009-07-04T11:00:00Z 2015-05-08T01:11:31Z RBS + RALDH II + TT RBS + RALDH II + TT true false _380_ 0 4533 9 Discontinued false RBS + RALDH II + TT false Suzanne Bartram component2009685 1 BBa_B0010 component2009687 1 BBa_B0012 component2009683 1 BBa_B0034 annotation2009683 1 BBa_B0034 range2009683 1 1 12 annotation2009685 1 BBa_B0010 range2009685 1 1563 1642 annotation2009687 1 BBa_B0012 range2009687 1 1651 1691 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K223009_sequence 1 aaagaggagaaatactagatgcccggcgaggtgaaggccgaccccgccgcgctcatggcctcgctgcagctcctgccgtcacccacgcccaacctcgagatcaagtacaccaagatctttattaacaatgaatggcagaactcagagagtgggagagtgttccctgtctgtaatccagccacaggagagcaagtgtgtgaagttcaagaagcagacaaggtggatatagacaaggcagtgcaggcggcccgcctggccttctctcttggttctgtgtggagaaggatggacgcttctgaaagaggacgtctgctggacaagcttgcagacttggtggaacgggacagggcaactcttgcaactatggaatcactaaatggcggtaagccattcctgcaagctttttacatcgatttgcagggagtcatcaaaaccctgaggtattatgcaggctgggctgataaaattcacggaatgaccattcctgtagatggagactattttaccttcacgagacatgagcccatcggagtgtgtggacagattatcccgtggaatttccccctgctgatgttcacctggaaaattgctcccgcactgtgctgtggtaacactgtggtcatcaagcctgcagagcagacaccactcagtgcactgtatatgggagccctcatcaaggaggctggctttccacccggggtcgtcaacattctgccagggtatgggccaacagcaggggcagcaatagcgtctcacattggcatagacaagattgcattcacaggctctactgaggttggaaagcttattcaagaagcagctggaagaagtaatttgaagagagtaactctggaacttggagggaaaagccctaatattatttttgcagatgctgacttggactacgctgtggagcaggctcaccagggtgtgttcttcaaccaaggccagtgctgcactgcggggtctcgaatctttgtggaggagtccatctatgaggaatttgtgaaaagaagtgtggaaagagccaagaggcgcattgtggggagcccctttgatcccactactgagcagggtcctcagattgataagaagcaatacaacaaaatcctggagctcatccagagcggtgtggccgagggcgccaagctcgagtgtggaggcaaaggactgggaaggaagggcttcttcattgagcctaccgtgttctccaacgttacagatgacatgcggatcgccaaggaggagatctttggtcctgtccaggaaattttgaggtttaagactatggatgaagttatagaaagagccaataactcagacttcgggcttgtagcggctgtcttcactaatgacatcaacaaggctctcatggtgtcctctgcaatgcaagctgggacagtttggatcaattgttacaacgccttaaatgctcagagccccttcggggggttcaagatgtctggaaatgggagagaaatgggtgagtttggcttacgggagtactcagaagtaaagaccgtgacggtgaagatcccccagaagaactcctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z